Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry
Abstract Background Marek’s disease (MD) is a chicken neoplastic disease, which brings huge economic losses to the global poultry industry. The wild type p53, a tumor suppressor gene, plays a key role in blocking cell cycle, promoting apoptosis, and maintaining the stability of the genome. However,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02520-2 |
| _version_ | 1819059547628634112 |
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| author | Huixia Zhang Mengda Liu Hui Zhang Shengliang Cao Yue Li Shengnan Jiang Yinuo Song Sidang Liu |
| author_facet | Huixia Zhang Mengda Liu Hui Zhang Shengliang Cao Yue Li Shengnan Jiang Yinuo Song Sidang Liu |
| author_sort | Huixia Zhang |
| collection | DOAJ |
| description | Abstract Background Marek’s disease (MD) is a chicken neoplastic disease, which brings huge economic losses to the global poultry industry. The wild type p53, a tumor suppressor gene, plays a key role in blocking cell cycle, promoting apoptosis, and maintaining the stability of the genome. However, the mutant p53 losses its tumor inhibitory role and become an oncogene when a mutation has happened. Results The mutation rate of p53 was 60% in the experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated p53 was expressed in various tumor tissues in an infected chicken. The mutant P53 proteins were notably accumulated in the cytoplasm due to the loss in the function of nuclear localization. Unlike the study on human cancer, the concentrations of P53 in the serums of MD infected chicken were significantly lower than the control group. Conclusions The p53 mutations were apparent in the development of MD. P53 and P53 antibody level in serum could be a useful marker in the diagnosis and surveillance of MD. |
| first_indexed | 2024-12-21T14:12:50Z |
| format | Article |
| id | doaj.art-c3f060abc1bc4669a748ae48b6df0b07 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T14:12:50Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-c3f060abc1bc4669a748ae48b6df0b072022-12-21T19:00:59ZengBMCBMC Veterinary Research1746-61482020-08-011611710.1186/s12917-020-02520-2Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultryHuixia Zhang0Mengda Liu1Hui Zhang2Shengliang Cao3Yue Li4Shengnan Jiang5Yinuo Song6Sidang Liu7College of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityChina Animal Health and Epidemiology CenterCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Shandong Agricultural UniversityAbstract Background Marek’s disease (MD) is a chicken neoplastic disease, which brings huge economic losses to the global poultry industry. The wild type p53, a tumor suppressor gene, plays a key role in blocking cell cycle, promoting apoptosis, and maintaining the stability of the genome. However, the mutant p53 losses its tumor inhibitory role and become an oncogene when a mutation has happened. Results The mutation rate of p53 was 60% in the experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated p53 was expressed in various tumor tissues in an infected chicken. The mutant P53 proteins were notably accumulated in the cytoplasm due to the loss in the function of nuclear localization. Unlike the study on human cancer, the concentrations of P53 in the serums of MD infected chicken were significantly lower than the control group. Conclusions The p53 mutations were apparent in the development of MD. P53 and P53 antibody level in serum could be a useful marker in the diagnosis and surveillance of MD.http://link.springer.com/article/10.1186/s12917-020-02520-2Marek’s diseasep53P53 antibody |
| spellingShingle | Huixia Zhang Mengda Liu Hui Zhang Shengliang Cao Yue Li Shengnan Jiang Yinuo Song Sidang Liu Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry BMC Veterinary Research Marek’s disease p53 P53 antibody |
| title | Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry |
| title_full | Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry |
| title_fullStr | Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry |
| title_full_unstemmed | Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry |
| title_short | Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry |
| title_sort | detection of p53 mutation and serum monitoring alert caused by marek s disease virus in poultry |
| topic | Marek’s disease p53 P53 antibody |
| url | http://link.springer.com/article/10.1186/s12917-020-02520-2 |
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