Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence
Abstract Background Chemotherapy can induce cellular senescence and a secretory phenotype characterized by an increased expression of inflammatory cytokines, such as IL‐6 and MCP‐1. Increased IL‐6 and MCP‐1 serum concentrations have been documented in dogs with lymphoma, but no studies have evaluate...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.633 |
| _version_ | 1819243242995056640 |
|---|---|
| author | Brittany L. Evans Joelle M. Fenger Greg Ballash Megan Brown |
| author_facet | Brittany L. Evans Joelle M. Fenger Greg Ballash Megan Brown |
| author_sort | Brittany L. Evans |
| collection | DOAJ |
| description | Abstract Background Chemotherapy can induce cellular senescence and a secretory phenotype characterized by an increased expression of inflammatory cytokines, such as IL‐6 and MCP‐1. Increased IL‐6 and MCP‐1 serum concentrations have been documented in dogs with lymphoma, but no studies have evaluated the effects of chemotherapy on cytokine concentrations. Objectives To measure IL‐6 and MCP‐1 in 16 client‐owned dogs with lymphoma, at baseline and before and after doxorubicin, as a potential marker for senescence and correlate cytokine concentrations with treatment response and toxicities. Methods Serum IL‐6 and MCP‐1 concentrations at baseline, 0‐h, 3‐h, 6‐h, 24‐h and 1 week post doxorubicin were measured using a canine ELISA. We hypothesized that IL‐6 and MCP‐1 concentrations would increase following doxorubicin as a result of induction of cellular senescence. Results IL‐6 concentrations were unchanged from baseline to 0‐h but significantly decreased 1 week post doxorubicin (p = 0.001) compared to 0–6 h (p = 0.045) and 24‐h (p = 0.001) time points. MCP‐1 concentrations significantly decreased from baseline to 0‐h (p = 0.003). Compared to 0–6 h, MCP‐1 concentrations transiently increased at 24‐h (p = 0.001) and decreased at 1 week (p = 0.014) post doxorubicin. Changes in IL‐6 and MCP‐1 concentrations did not correlate with leukocyte count, response to treatment or chemotherapy toxicities. Conclusions Changes in IL‐6 and MCP‐1 concentrations did not support doxorubicin‐induced cellular senescence or correlate with leukocyte count, response to treatment or chemotherapy toxicity. However, our results suggest that remission status and doxorubicin treatment may influence cytokine concentrations and future studies are warranted to investigate the role of these cytokines as biomarkers. |
| first_indexed | 2024-12-23T14:52:36Z |
| format | Article |
| id | doaj.art-c3f0a920e2444c41a23c896adb634da1 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-23T14:52:36Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-c3f0a920e2444c41a23c896adb634da12022-12-21T17:42:54ZengWileyVeterinary Medicine and Science2053-10952022-01-0181859610.1002/vms3.633Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescenceBrittany L. Evans0Joelle M. Fenger1Greg Ballash2Megan Brown3Department of Veterinary Clinical Sciences, College of Veterinary Medicine The Ohio State University Columbus Ohio USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine The Ohio State University Columbus Ohio USADepartment of Veterinary Preventative Medicine, College of Veterinary Medicine The Ohio State University Columbus Ohio USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine The Ohio State University Columbus Ohio USAAbstract Background Chemotherapy can induce cellular senescence and a secretory phenotype characterized by an increased expression of inflammatory cytokines, such as IL‐6 and MCP‐1. Increased IL‐6 and MCP‐1 serum concentrations have been documented in dogs with lymphoma, but no studies have evaluated the effects of chemotherapy on cytokine concentrations. Objectives To measure IL‐6 and MCP‐1 in 16 client‐owned dogs with lymphoma, at baseline and before and after doxorubicin, as a potential marker for senescence and correlate cytokine concentrations with treatment response and toxicities. Methods Serum IL‐6 and MCP‐1 concentrations at baseline, 0‐h, 3‐h, 6‐h, 24‐h and 1 week post doxorubicin were measured using a canine ELISA. We hypothesized that IL‐6 and MCP‐1 concentrations would increase following doxorubicin as a result of induction of cellular senescence. Results IL‐6 concentrations were unchanged from baseline to 0‐h but significantly decreased 1 week post doxorubicin (p = 0.001) compared to 0–6 h (p = 0.045) and 24‐h (p = 0.001) time points. MCP‐1 concentrations significantly decreased from baseline to 0‐h (p = 0.003). Compared to 0–6 h, MCP‐1 concentrations transiently increased at 24‐h (p = 0.001) and decreased at 1 week (p = 0.014) post doxorubicin. Changes in IL‐6 and MCP‐1 concentrations did not correlate with leukocyte count, response to treatment or chemotherapy toxicities. Conclusions Changes in IL‐6 and MCP‐1 concentrations did not support doxorubicin‐induced cellular senescence or correlate with leukocyte count, response to treatment or chemotherapy toxicity. However, our results suggest that remission status and doxorubicin treatment may influence cytokine concentrations and future studies are warranted to investigate the role of these cytokines as biomarkers.https://doi.org/10.1002/vms3.633caninechemotherapycytokinetoxicity |
| spellingShingle | Brittany L. Evans Joelle M. Fenger Greg Ballash Megan Brown Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence Veterinary Medicine and Science canine chemotherapy cytokine toxicity |
| title | Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| title_full | Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| title_fullStr | Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| title_full_unstemmed | Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| title_short | Serum IL‐6 and MCP‐1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| title_sort | serum il 6 and mcp 1 concentrations in dogs with lymphoma before and after doxorubicin treatment as a potential marker of cellular senescence |
| topic | canine chemotherapy cytokine toxicity |
| url | https://doi.org/10.1002/vms3.633 |
| work_keys_str_mv | AT brittanylevans serumil6andmcp1concentrationsindogswithlymphomabeforeandafterdoxorubicintreatmentasapotentialmarkerofcellularsenescence AT joellemfenger serumil6andmcp1concentrationsindogswithlymphomabeforeandafterdoxorubicintreatmentasapotentialmarkerofcellularsenescence AT gregballash serumil6andmcp1concentrationsindogswithlymphomabeforeandafterdoxorubicintreatmentasapotentialmarkerofcellularsenescence AT meganbrown serumil6andmcp1concentrationsindogswithlymphomabeforeandafterdoxorubicintreatmentasapotentialmarkerofcellularsenescence |