Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity
Inflammation is the first physiological defence mechanism against external and internal stimuli. The prolonged or inappropriate response of the immune system may lead to the persistent inflammatory response that can potentially become a basis for chronic diseases e.g., asthma, type II diabetes or ca...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3750 |
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| author | Małgorzata Kołtun-Jasion Paulina Sawulska Andrzej Patyra Marta Woźniak Marta Katarzyna Dudek Agnieszka Filipek Anna Karolina Kiss |
| author_facet | Małgorzata Kołtun-Jasion Paulina Sawulska Andrzej Patyra Marta Woźniak Marta Katarzyna Dudek Agnieszka Filipek Anna Karolina Kiss |
| author_sort | Małgorzata Kołtun-Jasion |
| collection | DOAJ |
| description | Inflammation is the first physiological defence mechanism against external and internal stimuli. The prolonged or inappropriate response of the immune system may lead to the persistent inflammatory response that can potentially become a basis for chronic diseases e.g., asthma, type II diabetes or cancer. An important role in the alleviation of inflammatory processes, as an adjunct to traditional pharmacological therapy, is attributed to phytotherapy, especially to raw materials with a long tradition of use, e.g., ash leaves. Despite their long-term use in phytotherapy, the specific mechanisms of action have not been confirmed in a sufficient number of biological or clinical studies. The aim of the study is a detailed phytochemical analysis of infusion and its fractions, isolation of pure compounds from the leaves of <i>Fraxinus excelsior</i> and evaluation of their effect on the secretion of anti-inflammatory cytokines (TNF-α, IL-6) and IL-10 receptor expression in an in vitro model of monocyte/macrophage cells isolated from peripheral blood. Methods: Phytochemical analysis was carried out by the UHPLC-DAD-ESI-MS/MS method. Monocytes/macrophages were isolated from human peripheral blood using density gradient centrifugation on Pancoll. After 24 h incubation with tested fractions/subfractions and pure compounds, cells or their supernatants were studied, respectively, on IL-10 receptor expression by flow cytometry and IL-6, TNF-α, IL-1β secretion by the ELISA test. Results were presented with respect to <i>Lipopolysaccharide</i> (LPS) control and positive control with dexamethasone. Results: The infusion, 20% and 50% methanolic fractions and their subfractions, as well as their dominating compounds, e.g., ligstroside, formoside and oleoacteoside isolated from the leaves, show the ability to increase the IL-10 receptor expression on the surface of monocyte/macrophage cells, stimulated by LPS, and to decrease the secretion of pro-inflammatory cytokines, e.g., TNF-α, IL-6. |
| first_indexed | 2024-03-11T08:42:16Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:42:16Z |
| publishDate | 2023-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c3f35bfa7f054777aea137159f6540792023-11-16T21:04:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244375010.3390/ijms24043750Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory ActivityMałgorzata Kołtun-Jasion0Paulina Sawulska1Andrzej Patyra2Marta Woźniak3Marta Katarzyna Dudek4Agnieszka Filipek5Anna Karolina Kiss6Department of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandStructural Studies Department, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza H. 112, 90-001 Łódź, PolandDepartment of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Pharmaceutical Biology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandInflammation is the first physiological defence mechanism against external and internal stimuli. The prolonged or inappropriate response of the immune system may lead to the persistent inflammatory response that can potentially become a basis for chronic diseases e.g., asthma, type II diabetes or cancer. An important role in the alleviation of inflammatory processes, as an adjunct to traditional pharmacological therapy, is attributed to phytotherapy, especially to raw materials with a long tradition of use, e.g., ash leaves. Despite their long-term use in phytotherapy, the specific mechanisms of action have not been confirmed in a sufficient number of biological or clinical studies. The aim of the study is a detailed phytochemical analysis of infusion and its fractions, isolation of pure compounds from the leaves of <i>Fraxinus excelsior</i> and evaluation of their effect on the secretion of anti-inflammatory cytokines (TNF-α, IL-6) and IL-10 receptor expression in an in vitro model of monocyte/macrophage cells isolated from peripheral blood. Methods: Phytochemical analysis was carried out by the UHPLC-DAD-ESI-MS/MS method. Monocytes/macrophages were isolated from human peripheral blood using density gradient centrifugation on Pancoll. After 24 h incubation with tested fractions/subfractions and pure compounds, cells or their supernatants were studied, respectively, on IL-10 receptor expression by flow cytometry and IL-6, TNF-α, IL-1β secretion by the ELISA test. Results were presented with respect to <i>Lipopolysaccharide</i> (LPS) control and positive control with dexamethasone. Results: The infusion, 20% and 50% methanolic fractions and their subfractions, as well as their dominating compounds, e.g., ligstroside, formoside and oleoacteoside isolated from the leaves, show the ability to increase the IL-10 receptor expression on the surface of monocyte/macrophage cells, stimulated by LPS, and to decrease the secretion of pro-inflammatory cytokines, e.g., TNF-α, IL-6.https://www.mdpi.com/1422-0067/24/4/3750<i>Fraxinus excelsior</i>ash leavesinflammation<i>Oleaceae</i>secoiridoidsphenylethanoids |
| spellingShingle | Małgorzata Kołtun-Jasion Paulina Sawulska Andrzej Patyra Marta Woźniak Marta Katarzyna Dudek Agnieszka Filipek Anna Karolina Kiss Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity International Journal of Molecular Sciences <i>Fraxinus excelsior</i> ash leaves inflammation <i>Oleaceae</i> secoiridoids phenylethanoids |
| title | Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity |
| title_full | Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity |
| title_fullStr | Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity |
| title_full_unstemmed | Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity |
| title_short | Bio-Guided Isolation of Compounds from <i>Fraxinus excelsior</i> Leaves with Anti-Inflammatory Activity |
| title_sort | bio guided isolation of compounds from i fraxinus excelsior i leaves with anti inflammatory activity |
| topic | <i>Fraxinus excelsior</i> ash leaves inflammation <i>Oleaceae</i> secoiridoids phenylethanoids |
| url | https://www.mdpi.com/1422-0067/24/4/3750 |
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