Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping
<p>Abstract</p> <p>Background</p> <p>The diversity of genotyping markers of <it>Plasmodium falciparum </it>depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (<it>msp2</it>) is greater in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-10-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/8/1/250 |
| _version_ | 1818564736020643840 |
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| author | Kiniboro Benson Lin Enmoore Valsangiacomo Francesca Schoepflin Sonja Mueller Ivo Felger Ingrid |
| author_facet | Kiniboro Benson Lin Enmoore Valsangiacomo Francesca Schoepflin Sonja Mueller Ivo Felger Ingrid |
| author_sort | Kiniboro Benson |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The diversity of genotyping markers of <it>Plasmodium falciparum </it>depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (<it>msp2</it>) is greater in areas of high compared to low endemicity, however, results for <it>msp1 </it>were inconsistent. These previous reports relied on low resolution genotyping techniques.</p> <p>Methods</p> <p>In the present study, a high-resolution capillary electrophoresis-based technique was applied to genotype samples from areas of different endemicity in Papua New Guinea and Tanzania. For both endemic settings, the diversity of <it>msp1 </it>and <it>msp2 </it>was investigated; the mean multiplicity of infection (MOI) and the F<sub>ST </sub>values were determined to investigate whether more accurate sizing generates different results.</p> <p>Results and Conclusion</p> <p>The results of the present study confirmed previous reports of a higher mean MOI for both marker genes and increased genetic diversity in areas of higher endemicity as estimated by the total number of distinct alleles for <it>msp2</it>. For <it>msp1 </it>a minor increase in diversity was observed. Measures of between population variance in allele frequencies (F<sub>ST</sub>) indicated little genetic differentiation for both marker genes between the two populations from different endemic settings. MOI adjusted for the probability of multiple infections sharing the same allele was estimated by using the <it>msp2 </it>allele frequency distribution and the distribution of observed numbers of concurrent infections. For the high-resolution typing technique applied in this study, this adjustment made little difference to the estimated mean MOI compared to the observed mean MOI.</p> |
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| format | Article |
| id | doaj.art-c3f3b254eaaa42c4a85ac6f99ea98bba |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-14T01:32:34Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-c3f3b254eaaa42c4a85ac6f99ea98bba2022-12-21T23:21:59ZengBMCMalaria Journal1475-28752009-10-018125010.1186/1475-2875-8-250Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotypingKiniboro BensonLin EnmooreValsangiacomo FrancescaSchoepflin SonjaMueller IvoFelger Ingrid<p>Abstract</p> <p>Background</p> <p>The diversity of genotyping markers of <it>Plasmodium falciparum </it>depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (<it>msp2</it>) is greater in areas of high compared to low endemicity, however, results for <it>msp1 </it>were inconsistent. These previous reports relied on low resolution genotyping techniques.</p> <p>Methods</p> <p>In the present study, a high-resolution capillary electrophoresis-based technique was applied to genotype samples from areas of different endemicity in Papua New Guinea and Tanzania. For both endemic settings, the diversity of <it>msp1 </it>and <it>msp2 </it>was investigated; the mean multiplicity of infection (MOI) and the F<sub>ST </sub>values were determined to investigate whether more accurate sizing generates different results.</p> <p>Results and Conclusion</p> <p>The results of the present study confirmed previous reports of a higher mean MOI for both marker genes and increased genetic diversity in areas of higher endemicity as estimated by the total number of distinct alleles for <it>msp2</it>. For <it>msp1 </it>a minor increase in diversity was observed. Measures of between population variance in allele frequencies (F<sub>ST</sub>) indicated little genetic differentiation for both marker genes between the two populations from different endemic settings. MOI adjusted for the probability of multiple infections sharing the same allele was estimated by using the <it>msp2 </it>allele frequency distribution and the distribution of observed numbers of concurrent infections. For the high-resolution typing technique applied in this study, this adjustment made little difference to the estimated mean MOI compared to the observed mean MOI.</p>http://www.malariajournal.com/content/8/1/250 |
| spellingShingle | Kiniboro Benson Lin Enmoore Valsangiacomo Francesca Schoepflin Sonja Mueller Ivo Felger Ingrid Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping Malaria Journal |
| title | Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping |
| title_full | Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping |
| title_fullStr | Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping |
| title_full_unstemmed | Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping |
| title_short | Comparison of <it>Plasmodium falciparum </it>allelic frequency distribution in different endemic settings by high-resolution genotyping |
| title_sort | comparison of it plasmodium falciparum it allelic frequency distribution in different endemic settings by high resolution genotyping |
| url | http://www.malariajournal.com/content/8/1/250 |
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