Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant
Chlorophyll contributes to tea coloration, which is an important factor in tea quality. Chlorophyll metabolism is induced by light, but the transcriptional regulation responsible for light-induced chlorophyll metabolism is largely unknown in tea leaves. Here, we characterized a chlorophyllase1 gene...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-12-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S246801412200139X |
| _version_ | 1797630632334983168 |
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| author | Weimin Liu Siyan Liu Kaiyue Zhang Mingwei Xie Haiwei Sun Xiaoqin Huang Lixia Zhang Min Li |
| author_facet | Weimin Liu Siyan Liu Kaiyue Zhang Mingwei Xie Haiwei Sun Xiaoqin Huang Lixia Zhang Min Li |
| author_sort | Weimin Liu |
| collection | DOAJ |
| description | Chlorophyll contributes to tea coloration, which is an important factor in tea quality. Chlorophyll metabolism is induced by light, but the transcriptional regulation responsible for light-induced chlorophyll metabolism is largely unknown in tea leaves. Here, we characterized a chlorophyllase1 gene CsCLH1 from young tea leaves and showed it is essential for chlorophyll metabolism, using transient overexpression and silencing in tea leaves and ectopic overexpression in Arabidopsis. CsCLH1 was significantly induced by high light. The DOF protein CsDOF3, an upstream direct regulator of CsCLH1, was also identified. Acting as a nuclear-localized transcriptional factor, CsDOF3 responded for light and repressed CsCLH1 transcription and increased chlorophyll content by directly binding to the AAAG cis-element in the CsCLH1 promoter. CsDOF3 was able to physically interact with the R2R3-MYB transcription factor CsMYB308 and interfere with transcriptional activity of CsCLH1. In addition, CsMYB308 binds to the CsCLH1 promoter to enhance CsCLH1 expression and decrease chlorophyll content. CsMYB308 and CsDOF3 act as an antagonistic complex to regulate CsCLH1 transcription and chlorophyll in young leaves. Collectively, the study adds to the understanding of the transcriptional regulation of chlorophyll in tea leaves in response to light and provides a basis for improving the appearance of tea. |
| first_indexed | 2024-03-11T11:10:54Z |
| format | Article |
| id | doaj.art-c3f4903d923d4b9f9439920ea3cdd43e |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-03-11T11:10:54Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-c3f4903d923d4b9f9439920ea3cdd43e2023-11-12T04:40:43ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412023-12-019611621176Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plantWeimin Liu0Siyan Liu1Kaiyue Zhang2Mingwei Xie3Haiwei Sun4Xiaoqin Huang5Lixia Zhang6Min Li7State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, ChinaTea Research Institute of Tai'an Academy of Agricultural Sciences, Tai'an, Shandong 271001, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, ChinaState Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; Corresponding authors.State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; Corresponding authors.Chlorophyll contributes to tea coloration, which is an important factor in tea quality. Chlorophyll metabolism is induced by light, but the transcriptional regulation responsible for light-induced chlorophyll metabolism is largely unknown in tea leaves. Here, we characterized a chlorophyllase1 gene CsCLH1 from young tea leaves and showed it is essential for chlorophyll metabolism, using transient overexpression and silencing in tea leaves and ectopic overexpression in Arabidopsis. CsCLH1 was significantly induced by high light. The DOF protein CsDOF3, an upstream direct regulator of CsCLH1, was also identified. Acting as a nuclear-localized transcriptional factor, CsDOF3 responded for light and repressed CsCLH1 transcription and increased chlorophyll content by directly binding to the AAAG cis-element in the CsCLH1 promoter. CsDOF3 was able to physically interact with the R2R3-MYB transcription factor CsMYB308 and interfere with transcriptional activity of CsCLH1. In addition, CsMYB308 binds to the CsCLH1 promoter to enhance CsCLH1 expression and decrease chlorophyll content. CsMYB308 and CsDOF3 act as an antagonistic complex to regulate CsCLH1 transcription and chlorophyll in young leaves. Collectively, the study adds to the understanding of the transcriptional regulation of chlorophyll in tea leaves in response to light and provides a basis for improving the appearance of tea.http://www.sciencedirect.com/science/article/pii/S246801412200139XTea plantChlorophyllCsCLH1CsDOF3 transcription factorCsMYB308 transcription factor |
| spellingShingle | Weimin Liu Siyan Liu Kaiyue Zhang Mingwei Xie Haiwei Sun Xiaoqin Huang Lixia Zhang Min Li Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant Horticultural Plant Journal Tea plant Chlorophyll CsCLH1 CsDOF3 transcription factor CsMYB308 transcription factor |
| title | Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant |
| title_full | Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant |
| title_fullStr | Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant |
| title_full_unstemmed | Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant |
| title_short | Chlorophyllase is transcriptionally regulated by CsMYB308/CsDOF3 in young leaves of tea plant |
| title_sort | chlorophyllase is transcriptionally regulated by csmyb308 csdof3 in young leaves of tea plant |
| topic | Tea plant Chlorophyll CsCLH1 CsDOF3 transcription factor CsMYB308 transcription factor |
| url | http://www.sciencedirect.com/science/article/pii/S246801412200139X |
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