Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections
Summary: The COVID-19 pandemic, driven by the SARS-CoV-2 virus and its variants, highlights the important role of understanding host-viral molecular interactions influencing infection outcomes. Alternative splicing post-infection can impact both host responses and viral replication. We analyzed RNA...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | English |
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Elsevier
2024-03-01
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| Colección: | iScience |
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| Acceso en línea: | http://www.sciencedirect.com/science/article/pii/S2589004224003985 |
| _version_ | 1827345354511613952 |
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| author | Sung-Gwon Lee Priscilla A. Furth Lothar Hennighausen Hye Kyung Lee |
| author_facet | Sung-Gwon Lee Priscilla A. Furth Lothar Hennighausen Hye Kyung Lee |
| author_sort | Sung-Gwon Lee |
| collection | DOAJ |
| description | Summary: The COVID-19 pandemic, driven by the SARS-CoV-2 virus and its variants, highlights the important role of understanding host-viral molecular interactions influencing infection outcomes. Alternative splicing post-infection can impact both host responses and viral replication. We analyzed RNA splicing patterns in immune cells across various SARS-CoV-2 variants, considering immunization status. Using a dataset of 190 RNA-seq samples from our prior studies, we observed a substantial deactivation of alternative splicing and RNA splicing-related genes in COVID-19 patients. The alterations varied significantly depending on the infecting variant and immunization history. Notably, Alpha or Beta-infected patients differed from controls, while Omicron-infected patients displayed a splicing profile closer to controls. Particularly, vaccinated Omicron-infected individuals showed a distinct dynamic in alternative splicing patterns not widely shared among other groups. Our findings underscore the intricate interplay between SARS-CoV-2 variants, vaccination-induced immunity, and alternative splicing, emphasizing the need for further investigations to deepen understanding and guide therapeutic development. |
| first_indexed | 2024-03-07T23:06:27Z |
| format | Article |
| id | doaj.art-c3f4ce4a05f347ecbc34f2444deb112c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-07T23:06:27Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c3f4ce4a05f347ecbc34f2444deb112c2024-02-22T04:53:13ZengElsevieriScience2589-00422024-03-01273109177Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infectionsSung-Gwon Lee0Priscilla A. Furth1Lothar Hennighausen2Hye Kyung Lee3Section of Genetics and Physiology, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USASection of Genetics and Physiology, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USASection of Genetics and Physiology, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USASection of Genetics and Physiology, Laboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USA; Corresponding authorSummary: The COVID-19 pandemic, driven by the SARS-CoV-2 virus and its variants, highlights the important role of understanding host-viral molecular interactions influencing infection outcomes. Alternative splicing post-infection can impact both host responses and viral replication. We analyzed RNA splicing patterns in immune cells across various SARS-CoV-2 variants, considering immunization status. Using a dataset of 190 RNA-seq samples from our prior studies, we observed a substantial deactivation of alternative splicing and RNA splicing-related genes in COVID-19 patients. The alterations varied significantly depending on the infecting variant and immunization history. Notably, Alpha or Beta-infected patients differed from controls, while Omicron-infected patients displayed a splicing profile closer to controls. Particularly, vaccinated Omicron-infected individuals showed a distinct dynamic in alternative splicing patterns not widely shared among other groups. Our findings underscore the intricate interplay between SARS-CoV-2 variants, vaccination-induced immunity, and alternative splicing, emphasizing the need for further investigations to deepen understanding and guide therapeutic development.http://www.sciencedirect.com/science/article/pii/S2589004224003985ImmunologyMolecular biologyVirology |
| spellingShingle | Sung-Gwon Lee Priscilla A. Furth Lothar Hennighausen Hye Kyung Lee Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections iScience Immunology Molecular biology Virology |
| title | Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections |
| title_full | Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections |
| title_fullStr | Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections |
| title_full_unstemmed | Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections |
| title_short | Variant- and vaccination-specific alternative splicing profiles in SARS-CoV-2 infections |
| title_sort | variant and vaccination specific alternative splicing profiles in sars cov 2 infections |
| topic | Immunology Molecular biology Virology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224003985 |
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