Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus
Epigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, th...
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| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/11/9201 |
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| author | Elena Lonati Gessica Sala Paolo Corbetta Stefania Pagliari Emanuela Cazzaniga Laura Botto Pierangela Rovellini Ilaria Bruni Paola Palestini Alessandra Bulbarelli |
| author_facet | Elena Lonati Gessica Sala Paolo Corbetta Stefania Pagliari Emanuela Cazzaniga Laura Botto Pierangela Rovellini Ilaria Bruni Paola Palestini Alessandra Bulbarelli |
| author_sort | Elena Lonati |
| collection | DOAJ |
| description | Epigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, the employment of a nutritional strategy to prevent the onset or supplement disease therapies included functional food consumption. Its formulation consists of the addition of a phytoextract enriched in bioactive molecules. A good candidate as an ingredient is the Cinnamon verum aqueous extract. Indeed, this extract, subjected to a process of gastrointestinal digestion simulation (INFOGEST), exhibits beneficial antioxidant and anti-inflammatory properties in an in vitro model of the inflamed intestinal barrier. Here, we deepen the study of the mechanisms related to the effect of digested cinnamon extract pre-treatment, showing a correlation between transepithelial electrical resistance (TEER) decrement and alterations in claudin-2 expression under Tumor necrosis factor-α/Interleukin-1β (TNF-α/IL-1) β cytokine administration. Our results show that pre-treatment with cinnamon extract prevents TEER loss by claudin-2 protein level regulation, influencing both gene transcription and autophagy-mediated degradation. Hence, cinnamon polyphenols and their metabolites probably work as mediators in gene regulation and receptor/pathway activation, leading to an adaptive response against renewed insults. |
| first_indexed | 2024-03-11T03:06:56Z |
| format | Article |
| id | doaj.art-c3f685ebaec04f8ea55da2a020c58eed |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:06:56Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c3f685ebaec04f8ea55da2a020c58eed2023-11-18T07:55:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012411920110.3390/ijms24119201Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory StimulusElena Lonati0Gessica Sala1Paolo Corbetta2Stefania Pagliari3Emanuela Cazzaniga4Laura Botto5Pierangela Rovellini6Ilaria Bruni7Paola Palestini8Alessandra Bulbarelli9School of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalyMilan Center for Neuroscience (NeuroMI), School of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalyBicocca cEnter of Science and Technology for FOOD (BEST4FOOD), University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalyInnovhub Stazioni Sperimentali per l’Industria S.r.l., Via Giuseppe Colombo 79, 20133 Milan, ItalyBicocca cEnter of Science and Technology for FOOD (BEST4FOOD), University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, ItalyEpigenetic changes, host–gut microbiota interactions, and environmental factors contribute to inflammatory bowel disease (IBD) onset and progression. A healthy lifestyle may help to slow down the chronic or remitting/relapsing intestinal tract inflammation characteristic of IBD. In this scenario, the employment of a nutritional strategy to prevent the onset or supplement disease therapies included functional food consumption. Its formulation consists of the addition of a phytoextract enriched in bioactive molecules. A good candidate as an ingredient is the Cinnamon verum aqueous extract. Indeed, this extract, subjected to a process of gastrointestinal digestion simulation (INFOGEST), exhibits beneficial antioxidant and anti-inflammatory properties in an in vitro model of the inflamed intestinal barrier. Here, we deepen the study of the mechanisms related to the effect of digested cinnamon extract pre-treatment, showing a correlation between transepithelial electrical resistance (TEER) decrement and alterations in claudin-2 expression under Tumor necrosis factor-α/Interleukin-1β (TNF-α/IL-1) β cytokine administration. Our results show that pre-treatment with cinnamon extract prevents TEER loss by claudin-2 protein level regulation, influencing both gene transcription and autophagy-mediated degradation. Hence, cinnamon polyphenols and their metabolites probably work as mediators in gene regulation and receptor/pathway activation, leading to an adaptive response against renewed insults.https://www.mdpi.com/1422-0067/24/11/9201inflammationIBDintestinal barriercinnamon extractpolyphenolsin vitro digestion |
| spellingShingle | Elena Lonati Gessica Sala Paolo Corbetta Stefania Pagliari Emanuela Cazzaniga Laura Botto Pierangela Rovellini Ilaria Bruni Paola Palestini Alessandra Bulbarelli Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus International Journal of Molecular Sciences inflammation IBD intestinal barrier cinnamon extract polyphenols in vitro digestion |
| title | Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus |
| title_full | Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus |
| title_fullStr | Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus |
| title_full_unstemmed | Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus |
| title_short | Digested Cinnamon (<i>Cinnamomum verum</i> J. Presl) Bark Extract Modulates Claudin-2 Gene Expression and Protein Levels under TNFα/IL-1β Inflammatory Stimulus |
| title_sort | digested cinnamon i cinnamomum verum i j presl bark extract modulates claudin 2 gene expression and protein levels under tnfα il 1β inflammatory stimulus |
| topic | inflammation IBD intestinal barrier cinnamon extract polyphenols in vitro digestion |
| url | https://www.mdpi.com/1422-0067/24/11/9201 |
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