Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat
Objective: To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen. Methods: Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods: artificial vagina and electro-ejaculator. Samples fr...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2020-01-01
|
| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2020;volume=9;issue=5;spage=239;epage=246;aulast=Lukusa |
| _version_ | 1818330381560053760 |
|---|---|
| author | Kambulu Lukusa John Kabuba |
| author_facet | Kambulu Lukusa John Kabuba |
| author_sort | Kambulu Lukusa |
| collection | DOAJ |
| description | Objective: To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen.
Methods: Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods: artificial vagina and electro-ejaculator. Samples from each collection method were pooled and diluted with Tris-based extender. The pooled semen was divided into two cooling methods: slow cooling and fast cooling. Sperm motility and velocity, sperm plasma membrane integrity, sperm viability, mitochondrial membrane potential and DNA integrity were evaluated before dilution, immediately after cooling at 4 °C and at 24 h after freezing by using computer assisted sperm analyser method and epifluorescence microscope.
Results: Mean values for total motility, rapid-speed and progressive motile spermatozoa were significantly higher in semen collected with artificial vagina than electro-ejaculator (P<0.001). Slow cooling resulted in higher percentages of total motile, rapid-speed and progressive motile spermatozoa as compared to fast cooling (P<0.05). The post-thaw percentage of rapid-speed spermatozoa collected by artificial vagina in slow cooled sperm was significantly higher as compared to electro-ejaculator method (P<0.05). The mean values of curvilinear velocity and straight-line velocity were significantly higher in slow cooled semen obtained by artificial vagina (P<0.001). Similarly, the combination of artificial vagina and slow cooling resulted in significantly higher post-thaw percentages of straight-line velocity, average-path velocity, sperm plasma membrane integrity, viability and acrosome integrity (P<0.01). No significant differences were observed in slow cooling between artificial vagina and electro-ejaculator in terms of post-thaw sperm mitochondrial membrane potential and DNA integrity (P>0.05).
Conclusions: The use of artificial vagina and slow cooling rate in cryopreservation protocol improves the cooled and post-thaw sperm quality of Saanen buck semen. |
| first_indexed | 2024-12-13T13:03:03Z |
| format | Article |
| id | doaj.art-c3f946d5cdb042e4bae5485b92791387 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-12-13T13:03:03Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-c3f946d5cdb042e4bae5485b927913872022-12-21T23:44:56ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192020-01-019523924610.4103/2305-0500.294666Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goatKambulu LukusaJohn KabubaObjective: To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen. Methods: Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods: artificial vagina and electro-ejaculator. Samples from each collection method were pooled and diluted with Tris-based extender. The pooled semen was divided into two cooling methods: slow cooling and fast cooling. Sperm motility and velocity, sperm plasma membrane integrity, sperm viability, mitochondrial membrane potential and DNA integrity were evaluated before dilution, immediately after cooling at 4 °C and at 24 h after freezing by using computer assisted sperm analyser method and epifluorescence microscope. Results: Mean values for total motility, rapid-speed and progressive motile spermatozoa were significantly higher in semen collected with artificial vagina than electro-ejaculator (P<0.001). Slow cooling resulted in higher percentages of total motile, rapid-speed and progressive motile spermatozoa as compared to fast cooling (P<0.05). The post-thaw percentage of rapid-speed spermatozoa collected by artificial vagina in slow cooled sperm was significantly higher as compared to electro-ejaculator method (P<0.05). The mean values of curvilinear velocity and straight-line velocity were significantly higher in slow cooled semen obtained by artificial vagina (P<0.001). Similarly, the combination of artificial vagina and slow cooling resulted in significantly higher post-thaw percentages of straight-line velocity, average-path velocity, sperm plasma membrane integrity, viability and acrosome integrity (P<0.01). No significant differences were observed in slow cooling between artificial vagina and electro-ejaculator in terms of post-thaw sperm mitochondrial membrane potential and DNA integrity (P>0.05). Conclusions: The use of artificial vagina and slow cooling rate in cryopreservation protocol improves the cooled and post-thaw sperm quality of Saanen buck semen.http://www.apjr.net/article.asp?issn=2305-0500;year=2020;volume=9;issue=5;spage=239;epage=246;aulast=Lukusaartificial vagina; electro-ejaculator; sperm characteristics; cryopreservation |
| spellingShingle | Kambulu Lukusa John Kabuba Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat Asian Pacific Journal of Reproduction artificial vagina; electro-ejaculator; sperm characteristics; cryopreservation |
| title | Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat |
| title_full | Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat |
| title_fullStr | Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat |
| title_full_unstemmed | Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat |
| title_short | Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat |
| title_sort | semen collection methods and cooling rates affect post thaw sperm motility and kinematic parameters of saanen goat |
| topic | artificial vagina; electro-ejaculator; sperm characteristics; cryopreservation |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2020;volume=9;issue=5;spage=239;epage=246;aulast=Lukusa |
| work_keys_str_mv | AT kambululukusa semencollectionmethodsandcoolingratesaffectpostthawspermmotilityandkinematicparametersofsaanengoat AT johnkabuba semencollectionmethodsandcoolingratesaffectpostthawspermmotilityandkinematicparametersofsaanengoat |