Bacillus cereus contamination in infant formula: a study in food and drug control laboratory
Background: Bacillus cereus spores distribute widely in nature and can be isolated from different kinds of foods. This bacterium can produce diarrhea and emetic enterotoxins and syndromes. As infants are known to be more susceptible to B. cereus infection due to their incomplete intestinal flora and...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2007-10-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4209.pdf&manuscript_id=4209 |
| _version_ | 1819211231864553472 |
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| author | Rahimifard N. Fatholahzadeh B. Pirali Hamedani M. Noory Z. Saadati SH. Zavar M. Pirouz B. Asghari SH. Khezripour M. Saberi S. |
| author_facet | Rahimifard N. Fatholahzadeh B. Pirali Hamedani M. Noory Z. Saadati SH. Zavar M. Pirouz B. Asghari SH. Khezripour M. Saberi S. |
| author_sort | Rahimifard N. |
| collection | DOAJ |
| description | Background: Bacillus cereus spores distribute widely in nature and can be isolated from different kinds of foods. This bacterium can produce diarrhea and emetic enterotoxins and syndromes. As infants are known to be more susceptible to B. cereus infection due to their incomplete intestinal flora and fast growth of this bacterium during consumption, it is very important to investigate the presence of B. cereus in infant formula and possible pathogenicity of this microorganism in infants.  Methods: In this study, 60 samples of infant formula were examined for the presence of B. cereus. From a 1/10 dilution of each sample, a total amount of 1 ml was inoculated onto four phenol red agar plates containing mannitol, egg yolk emulsion and polymyxin B sulfate. The plates were incubated at 30°C for 24 hours. Confirmation tests were then performed on suspected colonies.Results: Among the 60 samples, 11 samples had more than 10 cfu/g, four of which contained more than 102 cfu/g. The other 49 samples showed less than 10 cfu/g of B. cereus.  Conclusions: We suggest that for infant formula the maximum microbial limit be reduced to less than 10 cfu/g to control B. cereus contamination and to prevent infection in infants. For this purpose, infant formula should be tested by the method and confirmation tests used in this study. In addition, susceptibility to penicillin, ß-hemolysis and growth rate at 45ºC could also be performed. |
| first_indexed | 2024-12-23T06:23:47Z |
| format | Article |
| id | doaj.art-c3faa32785694be7bf4f5e8b40ab524c |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-23T06:23:47Z |
| publishDate | 2007-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-c3faa32785694be7bf4f5e8b40ab524c2022-12-21T17:57:08ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222007-10-016586468Bacillus cereus contamination in infant formula: a study in food and drug control laboratoryRahimifard N.Fatholahzadeh B.Pirali Hamedani M.Noory Z.Saadati SH.Zavar M.Pirouz B.Asghari SH.Khezripour M.Saberi S.Background: Bacillus cereus spores distribute widely in nature and can be isolated from different kinds of foods. This bacterium can produce diarrhea and emetic enterotoxins and syndromes. As infants are known to be more susceptible to B. cereus infection due to their incomplete intestinal flora and fast growth of this bacterium during consumption, it is very important to investigate the presence of B. cereus in infant formula and possible pathogenicity of this microorganism in infants.  Methods: In this study, 60 samples of infant formula were examined for the presence of B. cereus. From a 1/10 dilution of each sample, a total amount of 1 ml was inoculated onto four phenol red agar plates containing mannitol, egg yolk emulsion and polymyxin B sulfate. The plates were incubated at 30°C for 24 hours. Confirmation tests were then performed on suspected colonies.Results: Among the 60 samples, 11 samples had more than 10 cfu/g, four of which contained more than 102 cfu/g. The other 49 samples showed less than 10 cfu/g of B. cereus.  Conclusions: We suggest that for infant formula the maximum microbial limit be reduced to less than 10 cfu/g to control B. cereus contamination and to prevent infection in infants. For this purpose, infant formula should be tested by the method and confirmation tests used in this study. In addition, susceptibility to penicillin, ß-hemolysis and growth rate at 45ºC could also be performed.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4209.pdf&manuscript_id=4209Bacillus cereusformula |
| spellingShingle | Rahimifard N. Fatholahzadeh B. Pirali Hamedani M. Noory Z. Saadati SH. Zavar M. Pirouz B. Asghari SH. Khezripour M. Saberi S. Bacillus cereus contamination in infant formula: a study in food and drug control laboratory Tehran University Medical Journal Bacillus cereus formula |
| title | Bacillus cereus contamination in infant formula: a study in food and drug control laboratory |
| title_full | Bacillus cereus contamination in infant formula: a study in food and drug control laboratory |
| title_fullStr | Bacillus cereus contamination in infant formula: a study in food and drug control laboratory |
| title_full_unstemmed | Bacillus cereus contamination in infant formula: a study in food and drug control laboratory |
| title_short | Bacillus cereus contamination in infant formula: a study in food and drug control laboratory |
| title_sort | bacillus cereus contamination in infant formula a study in food and drug control laboratory |
| topic | Bacillus cereus formula |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/4209.pdf&manuscript_id=4209 |
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