Dynamics of the IFT machinery at the ciliary tip
Intraflagellar transport (IFT) is essential for the elongation and maintenance of eukaryotic cilia and flagella. Due to the traffic jam of multiple trains at the ciliary tip, how IFT trains are remodeled in these turnaround zones cannot be determined by conventional imaging. Using PhotoGate, we visu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/28606 |
| _version_ | 1818024011524734976 |
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| author | Alexander Chien Sheng Min Shih Raqual Bower Douglas Tritschler Mary E Porter Ahmet Yildiz |
| author_facet | Alexander Chien Sheng Min Shih Raqual Bower Douglas Tritschler Mary E Porter Ahmet Yildiz |
| author_sort | Alexander Chien |
| collection | DOAJ |
| description | Intraflagellar transport (IFT) is essential for the elongation and maintenance of eukaryotic cilia and flagella. Due to the traffic jam of multiple trains at the ciliary tip, how IFT trains are remodeled in these turnaround zones cannot be determined by conventional imaging. Using PhotoGate, we visualized the full range of movement of single IFT trains and motors in Chlamydomonas flagella. Anterograde trains split apart and IFT complexes mix with each other at the tip to assemble retrograde trains. Dynein-1b is carried to the tip by kinesin-II as inactive cargo on anterograde trains. Unlike dynein-1b, kinesin-II detaches from IFT trains at the tip and diffuses in flagella. As the flagellum grows longer, diffusion delays return of kinesin-II to the basal body, depleting kinesin-II available for anterograde transport. Our results suggest that dissociation of kinesin-II from IFT trains serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas. |
| first_indexed | 2024-12-10T03:53:26Z |
| format | Article |
| id | doaj.art-c3faed7328fd4a65904343b07bae3f3a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:53:26Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c3faed7328fd4a65904343b07bae3f3a2022-12-22T02:03:11ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.28606Dynamics of the IFT machinery at the ciliary tipAlexander Chien0https://orcid.org/0000-0003-1101-6721Sheng Min Shih1Raqual Bower2Douglas Tritschler3Mary E Porter4Ahmet Yildiz5https://orcid.org/0000-0003-4792-174XBiophysics Graduate Group, University of California, Berkeley, Berkeley, United StatesPhysics Department, University of California, Berkeley, Berkeley, United StatesDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, United StatesDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, United StatesDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, United StatesBiophysics Graduate Group, University of California, Berkeley, Berkeley, United States; Physics Department, University of California, Berkeley, Berkeley, United States; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesIntraflagellar transport (IFT) is essential for the elongation and maintenance of eukaryotic cilia and flagella. Due to the traffic jam of multiple trains at the ciliary tip, how IFT trains are remodeled in these turnaround zones cannot be determined by conventional imaging. Using PhotoGate, we visualized the full range of movement of single IFT trains and motors in Chlamydomonas flagella. Anterograde trains split apart and IFT complexes mix with each other at the tip to assemble retrograde trains. Dynein-1b is carried to the tip by kinesin-II as inactive cargo on anterograde trains. Unlike dynein-1b, kinesin-II detaches from IFT trains at the tip and diffuses in flagella. As the flagellum grows longer, diffusion delays return of kinesin-II to the basal body, depleting kinesin-II available for anterograde transport. Our results suggest that dissociation of kinesin-II from IFT trains serves as a negative feedback mechanism that facilitates flagellar length control in Chlamydomonas.https://elifesciences.org/articles/28606intraflagellar transportkinesindyneinciliary length controlsingle molecule imagingphotogate |
| spellingShingle | Alexander Chien Sheng Min Shih Raqual Bower Douglas Tritschler Mary E Porter Ahmet Yildiz Dynamics of the IFT machinery at the ciliary tip eLife intraflagellar transport kinesin dynein ciliary length control single molecule imaging photogate |
| title | Dynamics of the IFT machinery at the ciliary tip |
| title_full | Dynamics of the IFT machinery at the ciliary tip |
| title_fullStr | Dynamics of the IFT machinery at the ciliary tip |
| title_full_unstemmed | Dynamics of the IFT machinery at the ciliary tip |
| title_short | Dynamics of the IFT machinery at the ciliary tip |
| title_sort | dynamics of the ift machinery at the ciliary tip |
| topic | intraflagellar transport kinesin dynein ciliary length control single molecule imaging photogate |
| url | https://elifesciences.org/articles/28606 |
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