Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness

Novel visual intelligent pH-indicator film was prepared from the eggshell membrane- gelatin, pectin, and anthocyanin pigment from butterfly pea flower (BP) (Clitoria ternatea). It was used as a real-time pH indicator for predicting food freshness. Eggshells are useful biowaste, gelatin has been extr...

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Main Authors: Gopika Puthussery Narayanan, Preetha Radhakrishnan, Pooja Baiju, Asmath Mubeena S
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Food Hydrocolloids for Health
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667025923000432
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author Gopika Puthussery Narayanan
Preetha Radhakrishnan
Pooja Baiju
Asmath Mubeena S
author_facet Gopika Puthussery Narayanan
Preetha Radhakrishnan
Pooja Baiju
Asmath Mubeena S
author_sort Gopika Puthussery Narayanan
collection DOAJ
description Novel visual intelligent pH-indicator film was prepared from the eggshell membrane- gelatin, pectin, and anthocyanin pigment from butterfly pea flower (BP) (Clitoria ternatea). It was used as a real-time pH indicator for predicting food freshness. Eggshells are useful biowaste, gelatin has been extracted from the eggshell membrane and was used for fabricating the film. The Anthocyanin content of BP extract was 198.3 mg g−1. The film's surface morphology and chemical nature were estimated using a Scanning electron microscope, Fourier transform infrared spectroscopy, and X-ray diffraction technique. The film was pH responsive and exhibited color variation ranging from shades of red, purple, blue, green, and yellow at different pH (1-13). The applicability of the developed pH- indicator film was studied on fresh Tilapia fish by monitoring its deterioration for a specific time period. The film showed a visible color change after seven days of storage at 4ºC from dark blue, bluish-grey to olive, and deep green. Change in the total volatile basic nitrogen (TVB-N) content and pH change had an effect on the color response of the film. The findings demonstrated that the fabricated pH indicator film proved to be pH sensitive and could be used to monitor fish freshness.
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spelling doaj.art-c3fe60aca250475d8130f28f4dcea8f12023-12-18T04:25:16ZengElsevierFood Hydrocolloids for Health2667-02592023-12-014100159Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish FreshnessGopika Puthussery Narayanan0Preetha Radhakrishnan1Pooja Baiju2Asmath Mubeena S3Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203Corresponding author.; Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203Novel visual intelligent pH-indicator film was prepared from the eggshell membrane- gelatin, pectin, and anthocyanin pigment from butterfly pea flower (BP) (Clitoria ternatea). It was used as a real-time pH indicator for predicting food freshness. Eggshells are useful biowaste, gelatin has been extracted from the eggshell membrane and was used for fabricating the film. The Anthocyanin content of BP extract was 198.3 mg g−1. The film's surface morphology and chemical nature were estimated using a Scanning electron microscope, Fourier transform infrared spectroscopy, and X-ray diffraction technique. The film was pH responsive and exhibited color variation ranging from shades of red, purple, blue, green, and yellow at different pH (1-13). The applicability of the developed pH- indicator film was studied on fresh Tilapia fish by monitoring its deterioration for a specific time period. The film showed a visible color change after seven days of storage at 4ºC from dark blue, bluish-grey to olive, and deep green. Change in the total volatile basic nitrogen (TVB-N) content and pH change had an effect on the color response of the film. The findings demonstrated that the fabricated pH indicator film proved to be pH sensitive and could be used to monitor fish freshness.http://www.sciencedirect.com/science/article/pii/S2667025923000432Eggshell membrane gelatinButterfly pea flowerAnthocyaninpH-indicator filmCasein/pectin-based edible film
spellingShingle Gopika Puthussery Narayanan
Preetha Radhakrishnan
Pooja Baiju
Asmath Mubeena S
Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
Food Hydrocolloids for Health
Eggshell membrane gelatin
Butterfly pea flower
Anthocyanin
pH-indicator film
Casein/pectin-based edible film
title Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
title_full Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
title_fullStr Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
title_full_unstemmed Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
title_short Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness
title_sort fabrication of butterfly pea flower anthocyanin incorporated colorimetric indicator film based on gelatin pectin for monitoring fish freshness
topic Eggshell membrane gelatin
Butterfly pea flower
Anthocyanin
pH-indicator film
Casein/pectin-based edible film
url http://www.sciencedirect.com/science/article/pii/S2667025923000432
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AT preetharadhakrishnan fabricationofbutterflypeafloweranthocyaninincorporatedcolorimetricindicatorfilmbasedongelatinpectinformonitoringfishfreshness
AT poojabaiju fabricationofbutterflypeafloweranthocyaninincorporatedcolorimetricindicatorfilmbasedongelatinpectinformonitoringfishfreshness
AT asmathmubeenas fabricationofbutterflypeafloweranthocyaninincorporatedcolorimetricindicatorfilmbasedongelatinpectinformonitoringfishfreshness