<i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’
Phalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via sec...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2016-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9692 |
| _version_ | 1818173739475402752 |
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| author | Dewi PRAMANIK Muhammad PRAMA YUFDY Herni SHINTIAVIRA Budi WINARTO |
| author_facet | Dewi PRAMANIK Muhammad PRAMA YUFDY Herni SHINTIAVIRA Budi WINARTO |
| author_sort | Dewi PRAMANIK |
| collection | DOAJ |
| description | Phalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, in vitro secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of Phalaenopsis ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoots on Murashige and Skoog (MS) medium containing 1.5 mg/L thidiazuron (TDZ) and 0.25 mg/L N6-benzylaminopurine (BAP) for ± 3 months. High secondary somatic embryo (SSE) formation up to 64.90% with 12.30 SSEs regenerated per embryo was determined on half-strength MS augmented with 0.5 mg/L BAP and 2.5 mg/L mT. The combination also stimulated the result of high multiplication rate of SSE formation, up to 10.1 fold on the third subculture, maintained low conversion rate of germinated-embryos down to 55% and improved qualified-growth of the germinated embryos. The mT treatment produced 86% survival plantlets with high qualified-performance. The system could be applied as an alternative method to step forward towards an improved propagation protocol, commercially efficient due to high productivity. Detail findings in each step were discussed. |
| first_indexed | 2024-12-11T19:33:18Z |
| format | Article |
| id | doaj.art-c3feabf1817e48908baae6388b72a109 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-11T19:33:18Z |
| publishDate | 2016-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-c3feabf1817e48908baae6388b72a1092022-12-22T00:53:13ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642016-03-0181626810.15835/nsb8196928193<i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’Dewi PRAMANIK0Muhammad PRAMA YUFDY1Herni SHINTIAVIRA2Budi WINARTO3Indonesian Ornamental Crops Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java-IndonesiaIndonesian Center for Horticultural Research and Development, Jln. Raya Ragunan, No. 29A, Pasar Minggu, Jakarta Selatan 12040, Jakarta-IndonesiaIndonesian Ornamental Crops Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java-IndonesiaIndonesian Ornamental Crop Research Institute, Jln. Raya Ciherang, Pacet-Cianjur 43253, West Java, IndonesiaPhalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, in vitro secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of Phalaenopsis ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoots on Murashige and Skoog (MS) medium containing 1.5 mg/L thidiazuron (TDZ) and 0.25 mg/L N6-benzylaminopurine (BAP) for ± 3 months. High secondary somatic embryo (SSE) formation up to 64.90% with 12.30 SSEs regenerated per embryo was determined on half-strength MS augmented with 0.5 mg/L BAP and 2.5 mg/L mT. The combination also stimulated the result of high multiplication rate of SSE formation, up to 10.1 fold on the third subculture, maintained low conversion rate of germinated-embryos down to 55% and improved qualified-growth of the germinated embryos. The mT treatment produced 86% survival plantlets with high qualified-performance. The system could be applied as an alternative method to step forward towards an improved propagation protocol, commercially efficient due to high productivity. Detail findings in each step were discussed.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9692 |
| spellingShingle | Dewi PRAMANIK Muhammad PRAMA YUFDY Herni SHINTIAVIRA Budi WINARTO <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ Notulae Scientia Biologicae |
| title | <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ |
| title_full | <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ |
| title_fullStr | <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ |
| title_full_unstemmed | <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ |
| title_short | <i>In Vitro</i> Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of <i>Phalaenopsis</i> ‘AMP 17’ |
| title_sort | lt i gt in vitro lt i gt secondary embryogenesis derived from meta topoline treatment on mass propagation of lt i gt phalaenopsis lt i gt amp 17 |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9692 |
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