Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment
In this work we report the new technical feasibility of combining a large-scale screw pressing facility with an industrial-scale pulsed electric field (PEF) system for a great increase in the yields of plant juice and crude protein extracted from freshly harvested blends of perennial ryegrass (Loliu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Future Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833522000600 |
| _version_ | 1811289190087786496 |
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| author | Xiaoai Guo Kemal Aganovic Ute Bindrich Andreas Juadjur Christian Hertel Edward Ebert Johannes große Macke Christoph Geil Volker Heinz |
| author_facet | Xiaoai Guo Kemal Aganovic Ute Bindrich Andreas Juadjur Christian Hertel Edward Ebert Johannes große Macke Christoph Geil Volker Heinz |
| author_sort | Xiaoai Guo |
| collection | DOAJ |
| description | In this work we report the new technical feasibility of combining a large-scale screw pressing facility with an industrial-scale pulsed electric field (PEF) system for a great increase in the yields of plant juice and crude protein extracted from freshly harvested blends of perennial ryegrass (Lolium perenne) (70%) and white clover (30%). A disk separator has been successfully utilized for continuous separation of 400 L of raw juice and successive protein precipitated at pH 5.0 and 60 °C. The yields of pressed juice and crude protein were increased by around 25% and 31%, respectively. The crude protein content reached 37.3 g/100 g in dry matter. In addition to the crude protein, the precipitated sediment contained minerals like Ca, K, Na, Mg and P, which are essential for health and promisingly interesting for future applications such as food alternatives. Microbial status, chemical compositions, macromolecular sizes and fat-protein microstructures of the samples collected at different process steps were explored, which allows for a better understanding of pilot-scale extraction processes and optimization. This study indicated a great potential for protein extraction from green biomass and for future food applications. |
| first_indexed | 2024-04-13T03:50:30Z |
| format | Article |
| id | doaj.art-c3ff5a1127744c8bac38c51cceb66363 |
| institution | Directory Open Access Journal |
| issn | 2666-8335 |
| language | English |
| last_indexed | 2024-04-13T03:50:30Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Future Foods |
| spelling | doaj.art-c3ff5a1127744c8bac38c51cceb663632022-12-22T03:03:50ZengElsevierFuture Foods2666-83352022-12-016100173Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatmentXiaoai Guo0Kemal Aganovic1Ute Bindrich2Andreas Juadjur3Christian Hertel4Edward Ebert5Johannes große Macke6Christoph Geil7Volker Heinz8German Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, Germany; Corresponding author.German Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyHof Ögens, Burhaver Strasse 22, 26969 Butjadingen, GermanyGerman Institute of Food Technologies (DIL e.V.), Prof.-von-Klitzing-Str. 7, 49610 Quakenbrück, GermanyIn this work we report the new technical feasibility of combining a large-scale screw pressing facility with an industrial-scale pulsed electric field (PEF) system for a great increase in the yields of plant juice and crude protein extracted from freshly harvested blends of perennial ryegrass (Lolium perenne) (70%) and white clover (30%). A disk separator has been successfully utilized for continuous separation of 400 L of raw juice and successive protein precipitated at pH 5.0 and 60 °C. The yields of pressed juice and crude protein were increased by around 25% and 31%, respectively. The crude protein content reached 37.3 g/100 g in dry matter. In addition to the crude protein, the precipitated sediment contained minerals like Ca, K, Na, Mg and P, which are essential for health and promisingly interesting for future applications such as food alternatives. Microbial status, chemical compositions, macromolecular sizes and fat-protein microstructures of the samples collected at different process steps were explored, which allows for a better understanding of pilot-scale extraction processes and optimization. This study indicated a great potential for protein extraction from green biomass and for future food applications.http://www.sciencedirect.com/science/article/pii/S2666833522000600Grass proteinProtein extractionGrass-clover blendPulsed electric field (PEF)Juice yieldDisc separator |
| spellingShingle | Xiaoai Guo Kemal Aganovic Ute Bindrich Andreas Juadjur Christian Hertel Edward Ebert Johannes große Macke Christoph Geil Volker Heinz Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment Future Foods Grass protein Protein extraction Grass-clover blend Pulsed electric field (PEF) Juice yield Disc separator |
| title | Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| title_full | Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| title_fullStr | Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| title_full_unstemmed | Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| title_short | Extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| title_sort | extraction of protein from juice blend of grass and clover pressed by a pilot pressing facility combined with a pulsed electric field treatment |
| topic | Grass protein Protein extraction Grass-clover blend Pulsed electric field (PEF) Juice yield Disc separator |
| url | http://www.sciencedirect.com/science/article/pii/S2666833522000600 |
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