Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry
To study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060180 |
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| author | Xiaomei SHA Lijun ZHANG Wenli JIANG Guangyao WANG Zuohua XIE Guotai HUANG Zongcai TU |
| author_facet | Xiaomei SHA Lijun ZHANG Wenli JIANG Guangyao WANG Zuohua XIE Guotai HUANG Zongcai TU |
| author_sort | Xiaomei SHA |
| collection | DOAJ |
| description | To study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the characteristic peptides of donkey-hide gelatin, bovine gelatin and porcine gelatin. The results showed that 18, 3 and 5 common characteristic peptides were accurately detected in the four kinds of donkey-hide gelatin lump for donkey-hide gelatin, bovine gelatin and porcine gelatin, respectively, with 10, 1 and 1 from the α1 chain and 8, 2 and 4 from the α2 chain. Meanwhile, the number of common characteristic peptides with hydroxylated modifications was 15, 2 and 4 respectively, while those without any modification groups were 3, 1 and 1. These common characteristic peptides would provide a basis for the HPLC-MS/MS traceable identification of the gelatin source in donkey-hide gelatin lump, which is of reference significance for the quality assurance of donkey-hide gelatin lump products. |
| first_indexed | 2024-04-11T16:07:46Z |
| format | Article |
| id | doaj.art-c4047b424c604621a0516e458c891e7a |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:07:46Z |
| publishDate | 2022-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-c4047b424c604621a0516e458c891e7a2022-12-22T04:14:46ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-02-014349710410.13386/j.issn1002-0306.20210601802021060180-4Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass SpectrometryXiaomei SHA0Lijun ZHANG1Wenli JIANG2Guangyao WANG3Zuohua XIE4Guotai HUANG5Zongcai TU6National R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaNational R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaNational R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaNational R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaJiangxi Deshang Pharmaceutical Co., Ltd., Zhangshu 331208, ChinaJiangxi Deshang Pharmaceutical Research Institute Co., Ltd., Zhangshu 331208, ChinaNational R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, ChinaTo study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the characteristic peptides of donkey-hide gelatin, bovine gelatin and porcine gelatin. The results showed that 18, 3 and 5 common characteristic peptides were accurately detected in the four kinds of donkey-hide gelatin lump for donkey-hide gelatin, bovine gelatin and porcine gelatin, respectively, with 10, 1 and 1 from the α1 chain and 8, 2 and 4 from the α2 chain. Meanwhile, the number of common characteristic peptides with hydroxylated modifications was 15, 2 and 4 respectively, while those without any modification groups were 3, 1 and 1. These common characteristic peptides would provide a basis for the HPLC-MS/MS traceable identification of the gelatin source in donkey-hide gelatin lump, which is of reference significance for the quality assurance of donkey-hide gelatin lump products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060180donkey-hide gelatinhigh-performance liquid chromatography-mass spectrometrytraceability identificationbovine gelatinporcine gelatin |
| spellingShingle | Xiaomei SHA Lijun ZHANG Wenli JIANG Guangyao WANG Zuohua XIE Guotai HUANG Zongcai TU Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry Shipin gongye ke-ji donkey-hide gelatin high-performance liquid chromatography-mass spectrometry traceability identification bovine gelatin porcine gelatin |
| title | Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_full | Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_fullStr | Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_full_unstemmed | Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_short | Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry |
| title_sort | identification of characteristic peptides of different gelatins in donkey hide gelatin lump by high performance liquid chromatography high resolution mass spectrometry |
| topic | donkey-hide gelatin high-performance liquid chromatography-mass spectrometry traceability identification bovine gelatin porcine gelatin |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060180 |
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