Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC
A novel, simple, precise and accurate method developed for the estimation of Lamivudine and tenofovir disoproxil fumarate (TDF) in bulk drug form has been established. Lamivudine and tenofovir are well known drugs and used in treatment of HIV- Ⅰ. The method was performed by using C18 column, ODS H...
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| Format: | Article |
| Language: | English |
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Creative Pharma Assent
2020-11-01
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| Series: | Journal of Applied Pharmaceutical Research |
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| Online Access: | https://japtronline.com/index.php/joapr/article/view/172 |
| _version_ | 1819140010408935424 |
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| author | Shweta Sharma Amar Deep Ankalgi Pooja Kaushal M. S. Ashawat |
| author_facet | Shweta Sharma Amar Deep Ankalgi Pooja Kaushal M. S. Ashawat |
| author_sort | Shweta Sharma |
| collection | DOAJ |
| description | A novel, simple, precise and accurate method developed for the estimation of Lamivudine and tenofovir disoproxil fumarate (TDF) in bulk drug form has been established. Lamivudine and tenofovir are well known drugs and used in treatment of HIV- Ⅰ. The method was performed by using C18 column, ODS Hypersil column with UV detection at 262nm by using Acetonitrile and water in ratio 55:45. The retention time was found to be 2.8 and 6.8 min for Lamivudine and tenofovir disoproxil fumarate (TDF). The linearity was found in range of 6- 14µg/ml for Lamivudine and 10- 50µg/ml for Tenofovir disoproxil fumarate with flow rate 1ml/min. the method was validated for linearity, accuracy, precision and robustness as per ICH guidelines. This method is suitable for simultaneous analysis for both the nucleoside analog reverse- transcriptase inhibitors |
| first_indexed | 2024-12-22T11:31:45Z |
| format | Article |
| id | doaj.art-c405453d912c4868b042aff5d4f4e4df |
| institution | Directory Open Access Journal |
| issn | 2348-0335 |
| language | English |
| last_indexed | 2024-12-22T11:31:45Z |
| publishDate | 2020-11-01 |
| publisher | Creative Pharma Assent |
| record_format | Article |
| series | Journal of Applied Pharmaceutical Research |
| spelling | doaj.art-c405453d912c4868b042aff5d4f4e4df2022-12-21T18:27:36ZengCreative Pharma AssentJournal of Applied Pharmaceutical Research2348-03352020-11-0184707610.18231/j.joapr.2020.v.8.i.4.70.76172Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLCShweta Sharma0Amar Deep Ankalgi1https://orcid.org/0000-0002-0775-9998Pooja Kaushal2https://orcid.org/0000-0003-4159-6990M. S. Ashawat3Department of Pharmaceutical Analysis and Quality Assurance, Laureate Institute of Pharmacy, Kathog, Jawalamukhi, Himachal Pradesh-176031Department of Pharmaceutical Analysis and Quality Assurance, Laureate Institute of Pharmacy, Kathog, Jawalamukhi, Himachal Pradesh-176031Department of Pharmaceutical Analysis and Quality Assurance, Laureate Institute of Pharmacy, Kathog, Jawalamukhi, Himachal Pradesh-176031Department of Pharmaceutical Analysis and Quality Assurance, Laureate Institute of Pharmacy, Kathog, Jawalamukhi, Himachal Pradesh-176031A novel, simple, precise and accurate method developed for the estimation of Lamivudine and tenofovir disoproxil fumarate (TDF) in bulk drug form has been established. Lamivudine and tenofovir are well known drugs and used in treatment of HIV- Ⅰ. The method was performed by using C18 column, ODS Hypersil column with UV detection at 262nm by using Acetonitrile and water in ratio 55:45. The retention time was found to be 2.8 and 6.8 min for Lamivudine and tenofovir disoproxil fumarate (TDF). The linearity was found in range of 6- 14µg/ml for Lamivudine and 10- 50µg/ml for Tenofovir disoproxil fumarate with flow rate 1ml/min. the method was validated for linearity, accuracy, precision and robustness as per ICH guidelines. This method is suitable for simultaneous analysis for both the nucleoside analog reverse- transcriptase inhibitorshttps://japtronline.com/index.php/joapr/article/view/172lamivudinetenofovir disoproxil fumarateanti-hivrp-hplc |
| spellingShingle | Shweta Sharma Amar Deep Ankalgi Pooja Kaushal M. S. Ashawat Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC Journal of Applied Pharmaceutical Research lamivudine tenofovir disoproxil fumarate anti-hiv rp-hplc |
| title | Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC |
| title_full | Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC |
| title_fullStr | Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC |
| title_full_unstemmed | Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC |
| title_short | Method development and validation for multi-component analysis of lamivudine & tenofovir disoproxil fumarate in bulk drug by UV-visible spectrophotometer & RP-HPLC |
| title_sort | method development and validation for multi component analysis of lamivudine tenofovir disoproxil fumarate in bulk drug by uv visible spectrophotometer rp hplc |
| topic | lamivudine tenofovir disoproxil fumarate anti-hiv rp-hplc |
| url | https://japtronline.com/index.php/joapr/article/view/172 |
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