Visualization of the Nucleolus Using Ethynyl Uridine
Thanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2′-deoxyuridine (EdU), and nascent RNA by 5-ethynyl...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00177/full |
| _version_ | 1818928556658393088 |
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| author | Martina Dvořáčková Jiří Fajkus Jiří Fajkus Jiří Fajkus |
| author_facet | Martina Dvořáčková Jiří Fajkus Jiří Fajkus Jiří Fajkus |
| author_sort | Martina Dvořáčková |
| collection | DOAJ |
| description | Thanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2′-deoxyuridine (EdU), and nascent RNA by 5-ethynyl uridine (EU). While the labeling of replicating DNA by EdU has already been well established and further exploited in plants, the use of EU to reveal nascent RNA has not been developed to such an extent. In this article, we present a protocol for labeling of nucleolar RNA transcripts using EU and show that EU effectively highlights the nucleolus. The method is advantageous, because the need to prepare transgenic plants expressing fluorescently tagged nucleolar components when the nucleolus has to be visualized can be avoided. |
| first_indexed | 2024-12-20T03:30:47Z |
| format | Article |
| id | doaj.art-c405f3ba38324b0e963c7beb6052deac |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T03:30:47Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c405f3ba38324b0e963c7beb6052deac2022-12-21T19:55:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-02-01910.3389/fpls.2018.00177306352Visualization of the Nucleolus Using Ethynyl UridineMartina Dvořáčková0Jiří Fajkus1Jiří Fajkus2Jiří Fajkus3Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, CzechiaMendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, CzechiaLaboratory of Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, CzechiaInstitute of Biophysics, Academy of Sciences of the Czech Republic, Brno, CzechiaThanks to recent innovative methodologies, key cellular processes such as replication or transcription can be visualized directly in situ in intact tissues. Many studies use so-called click iT chemistry where nascent DNA can be tracked by 5-ethynyl-2′-deoxyuridine (EdU), and nascent RNA by 5-ethynyl uridine (EU). While the labeling of replicating DNA by EdU has already been well established and further exploited in plants, the use of EU to reveal nascent RNA has not been developed to such an extent. In this article, we present a protocol for labeling of nucleolar RNA transcripts using EU and show that EU effectively highlights the nucleolus. The method is advantageous, because the need to prepare transgenic plants expressing fluorescently tagged nucleolar components when the nucleolus has to be visualized can be avoided.http://journal.frontiersin.org/article/10.3389/fpls.2018.00177/fullnucleolusnucleustranscriptionArabidopsis thalianaclick iT |
| spellingShingle | Martina Dvořáčková Jiří Fajkus Jiří Fajkus Jiří Fajkus Visualization of the Nucleolus Using Ethynyl Uridine Frontiers in Plant Science nucleolus nucleus transcription Arabidopsis thaliana click iT |
| title | Visualization of the Nucleolus Using Ethynyl Uridine |
| title_full | Visualization of the Nucleolus Using Ethynyl Uridine |
| title_fullStr | Visualization of the Nucleolus Using Ethynyl Uridine |
| title_full_unstemmed | Visualization of the Nucleolus Using Ethynyl Uridine |
| title_short | Visualization of the Nucleolus Using Ethynyl Uridine |
| title_sort | visualization of the nucleolus using ethynyl uridine |
| topic | nucleolus nucleus transcription Arabidopsis thaliana click iT |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00177/full |
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