Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i>
<i>Ewingella americana</i> is a cosmopolitan bacterial pathogen that has been isolated from many hosts. Here, we sequenced a high-quality genome of <i>E. americana</i> B6-1 isolated from <i>Flammulina filiformis</i>, an important cultivated mushroom, performed a c...
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MDPI AG
2020-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/5/330 |
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| author | Zhenghui Liu Hongyan Sheng Benjamin Azu Okorley Yu Li Frederick Leo Sossah |
| author_facet | Zhenghui Liu Hongyan Sheng Benjamin Azu Okorley Yu Li Frederick Leo Sossah |
| author_sort | Zhenghui Liu |
| collection | DOAJ |
| description | <i>Ewingella americana</i> is a cosmopolitan bacterial pathogen that has been isolated from many hosts. Here, we sequenced a high-quality genome of <i>E. americana</i> B6-1 isolated from <i>Flammulina filiformis</i>, an important cultivated mushroom, performed a comparative genomic analysis with four other <i>E. americana</i> strains from various origins, and tested the susceptibility of B6-1 to antibiotics. The genome size, predicted genes, and GC (guanine-cytosine) content of B6-1 was 4.67 Mb, 4301, and 53.80%, respectively. The origin of the strains did not significantly affect the phylogeny, but mobile genetic elements shaped the evolution of the genus <i>Ewingella</i>. The strains encoded a set of common genes for type secretion, virulence effectors, CAZymes, and toxins required for pathogenicity in all hosts. They also had antibiotic resistance, pigments to suppress or evade host defense responses, as well as genes for adaptation to different environmental conditions, including temperature, oxidation, and nutrients. These findings provide a better understanding of the virulence, antibiotic resistance, and host adaptation strategies of <i>Ewingella</i>, and they also contribute to the development of effective control strategies. |
| first_indexed | 2024-03-10T20:10:48Z |
| format | Article |
| id | doaj.art-c4064a97aa634cd598d1918592076535 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:10:48Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-c4064a97aa634cd598d19185920765352023-11-19T22:57:07ZengMDPI AGPathogens2076-08172020-04-019533010.3390/pathogens9050330Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i>Zhenghui Liu0Hongyan Sheng1Benjamin Azu Okorley2Yu Li3Frederick Leo Sossah4Department of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaDepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USAEngineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, ChinaEngineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, ChinaEngineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China<i>Ewingella americana</i> is a cosmopolitan bacterial pathogen that has been isolated from many hosts. Here, we sequenced a high-quality genome of <i>E. americana</i> B6-1 isolated from <i>Flammulina filiformis</i>, an important cultivated mushroom, performed a comparative genomic analysis with four other <i>E. americana</i> strains from various origins, and tested the susceptibility of B6-1 to antibiotics. The genome size, predicted genes, and GC (guanine-cytosine) content of B6-1 was 4.67 Mb, 4301, and 53.80%, respectively. The origin of the strains did not significantly affect the phylogeny, but mobile genetic elements shaped the evolution of the genus <i>Ewingella</i>. The strains encoded a set of common genes for type secretion, virulence effectors, CAZymes, and toxins required for pathogenicity in all hosts. They also had antibiotic resistance, pigments to suppress or evade host defense responses, as well as genes for adaptation to different environmental conditions, including temperature, oxidation, and nutrients. These findings provide a better understanding of the virulence, antibiotic resistance, and host adaptation strategies of <i>Ewingella</i>, and they also contribute to the development of effective control strategies.https://www.mdpi.com/2076-0817/9/5/330<i>Ewingella americana</i>brown rot diseaseneedle mushroomwhole-genome sequencingantibiotic resistancevirulence genes |
| spellingShingle | Zhenghui Liu Hongyan Sheng Benjamin Azu Okorley Yu Li Frederick Leo Sossah Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> Pathogens <i>Ewingella americana</i> brown rot disease needle mushroom whole-genome sequencing antibiotic resistance virulence genes |
| title | Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> |
| title_full | Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> |
| title_fullStr | Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> |
| title_full_unstemmed | Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> |
| title_short | Comparative Genomic Analysis Provides Insights into the Phylogeny, Resistome, Virulome, and Host Adaptation in the Genus <i>Ewingella</i> |
| title_sort | comparative genomic analysis provides insights into the phylogeny resistome virulome and host adaptation in the genus i ewingella i |
| topic | <i>Ewingella americana</i> brown rot disease needle mushroom whole-genome sequencing antibiotic resistance virulence genes |
| url | https://www.mdpi.com/2076-0817/9/5/330 |
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