No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study
Despite the increasing epidemiological evidence that the <i>Onchocerca volvulus</i> parasite is strongly associated with epilepsy in children, hence the name onchocerciasis-associated epilepsy (OAE), the pathophysiological mechanism of OAE remains to be elucidated. In June 2014, children...
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MDPI AG
2021-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/7/787 |
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| author | Michael Roach Adrian Cantu Melissa Krizia Vieri Matthew Cotten Paul Kellam My Phan Lia van der Hoek Michel Mandro Floribert Tepage Germain Mambandu Gisele Musinya Anne Laudisoit Robert Colebunders Robert Edwards John L. Mokili |
| author_facet | Michael Roach Adrian Cantu Melissa Krizia Vieri Matthew Cotten Paul Kellam My Phan Lia van der Hoek Michel Mandro Floribert Tepage Germain Mambandu Gisele Musinya Anne Laudisoit Robert Colebunders Robert Edwards John L. Mokili |
| author_sort | Michael Roach |
| collection | DOAJ |
| description | Despite the increasing epidemiological evidence that the <i>Onchocerca volvulus</i> parasite is strongly associated with epilepsy in children, hence the name onchocerciasis-associated epilepsy (OAE), the pathophysiological mechanism of OAE remains to be elucidated. In June 2014, children with unprovoked convulsive epilepsy and healthy controls were enrolled in a case control study in Titule, Bas-Uélé Province in the Democratic Republic of the Congo (DRC) to identify risk factors for epilepsy. Using a subset of samples collected from individuals enrolled in this study (16 persons with OAE and 9 controls) plasma, buffy coat, and cerebrospinal fluid (CSF) were subjected to random-primed next-generation sequencing. The resulting sequences were analyzed using sensitive computational methods to identify viral DNA and RNA sequences. <i>Anneloviridae, Flaviviridae, Hepadnaviridae</i> (<i>Hepatitis B virus</i>), <i>Herpesviridae</i>, <i>Papillomaviridae, Polyomaviridae</i> (Human polyomavirus), and <i>Virgaviridae</i> were identified in cases and in controls. Not unexpectedly, a variety of bacteriophages were also detected in all cases and controls. However, none of the identified viral sequences were found enriched in OAE cases, which was our criteria for agents that might play a role in the etiology or pathogenesis of OAE. |
| first_indexed | 2024-03-10T10:11:01Z |
| format | Article |
| id | doaj.art-c406e87166b54579acb5a7b2018bc3d9 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T10:11:01Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-c406e87166b54579acb5a7b2018bc3d92023-11-22T01:14:40ZengMDPI AGPathogens2076-08172021-06-0110778710.3390/pathogens10070787No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control StudyMichael Roach0Adrian Cantu1Melissa Krizia Vieri2Matthew Cotten3Paul Kellam4My Phan5Lia van der Hoek6Michel Mandro7Floribert Tepage8Germain Mambandu9Gisele Musinya10Anne Laudisoit11Robert Colebunders12Robert Edwards13John L. Mokili14College of Science and Engineering, Flinders University, Adelaide, SA 5001, AustraliaComputational Sciences Research Center, Biology Department, San Diego State University, San Diego, CA 92182, USAGlobal Health Institute, University of Antwerp, 2160 Antwerp, BelgiumWellcome Trust Sanger Institute, Hinxton CB10 1RQ, UKMRC/UVRI and London School of Hygiene and Tropical Medicine, Entebbe, UgandaMRC/UVRI and London School of Hygiene and Tropical Medicine, Entebbe, UgandaLaboratory of Experimental Virology, Department of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, 1012 WX Amsterdam, The NetherlandsProvincial Health Division Ituri, Ministry of Health, Ituri, CongoProvincial Health Division Bas Uélé, Ministry of Health, Bas Uélé, CongoProvincial Health Division Tshopo, Ministry of Health, Tshopo, CongoMédecins Sans Frontières, Bunia, CongoEcohealth Alliance, New York, NY 10018, USAGlobal Health Institute, University of Antwerp, 2160 Antwerp, BelgiumCollege of Science and Engineering, Flinders University, Adelaide, SA 5001, AustraliaViral Information Institute, Biology Department, San Diego State University, San Diego, CA 92182, USADespite the increasing epidemiological evidence that the <i>Onchocerca volvulus</i> parasite is strongly associated with epilepsy in children, hence the name onchocerciasis-associated epilepsy (OAE), the pathophysiological mechanism of OAE remains to be elucidated. In June 2014, children with unprovoked convulsive epilepsy and healthy controls were enrolled in a case control study in Titule, Bas-Uélé Province in the Democratic Republic of the Congo (DRC) to identify risk factors for epilepsy. Using a subset of samples collected from individuals enrolled in this study (16 persons with OAE and 9 controls) plasma, buffy coat, and cerebrospinal fluid (CSF) were subjected to random-primed next-generation sequencing. The resulting sequences were analyzed using sensitive computational methods to identify viral DNA and RNA sequences. <i>Anneloviridae, Flaviviridae, Hepadnaviridae</i> (<i>Hepatitis B virus</i>), <i>Herpesviridae</i>, <i>Papillomaviridae, Polyomaviridae</i> (Human polyomavirus), and <i>Virgaviridae</i> were identified in cases and in controls. Not unexpectedly, a variety of bacteriophages were also detected in all cases and controls. However, none of the identified viral sequences were found enriched in OAE cases, which was our criteria for agents that might play a role in the etiology or pathogenesis of OAE.https://www.mdpi.com/2076-0817/10/7/787epilepsynodding syndromeonchocerciasisvirusesDemocratic Republic of Congo |
| spellingShingle | Michael Roach Adrian Cantu Melissa Krizia Vieri Matthew Cotten Paul Kellam My Phan Lia van der Hoek Michel Mandro Floribert Tepage Germain Mambandu Gisele Musinya Anne Laudisoit Robert Colebunders Robert Edwards John L. Mokili No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study Pathogens epilepsy nodding syndrome onchocerciasis viruses Democratic Republic of Congo |
| title | No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study |
| title_full | No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study |
| title_fullStr | No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study |
| title_full_unstemmed | No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study |
| title_short | No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study |
| title_sort | no evidence known viruses play a role in the pathogenesis of onchocerciasis associated epilepsy an explorative metagenomic case control study |
| topic | epilepsy nodding syndrome onchocerciasis viruses Democratic Republic of Congo |
| url | https://www.mdpi.com/2076-0817/10/7/787 |
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