Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace

Statistical experimental designs were applied for the optimization of medium constituents for soluble dietary fiber (SDF) production by Ganoderma lucidum in solid state fermentation (SSF) using lotus root pomace (LRP) as the substrate. Factors including wheat bran, MgSO4, FeSO4 and VB1 were identif...

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Main Authors: YONGQI YIN, MENGYU LIU, JINXIAN WU, RUNQIANG YANG, ZHENXIN GU
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2014-10-01
Series:The Indian Journal of Agricultural Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAgS/article/view/44195
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author YONGQI YIN
MENGYU LIU
JINXIAN WU
RUNQIANG YANG
ZHENXIN GU
author_facet YONGQI YIN
MENGYU LIU
JINXIAN WU
RUNQIANG YANG
ZHENXIN GU
author_sort YONGQI YIN
collection DOAJ
description Statistical experimental designs were applied for the optimization of medium constituents for soluble dietary fiber (SDF) production by Ganoderma lucidum in solid state fermentation (SSF) using lotus root pomace (LRP) as the substrate. Factors including wheat bran, MgSO4, FeSO4 and VB1 were identified by Plackett-Burman design (PBD) as significant for SDF production. The path of steepest ascent design (SAD) was undertaken to determine the optimal region of four significant factors. To determine the optimal values of the significant variables, Box-Behnken design (BBD) and response surface analysis were employed. The optimum conditions were found to be the 5g LRP, 1.77 g wheat bran, 0.029 g/L MgSO4, 0.011 g/L FeSO4 and 0.052 g/L VB1. SDF production at these optimum conditions was 0.113 g/g DW substrate. Compositional analysis showed that SDF was composed of rhamnose, xylose, mannose, galactose and glucose in a molar ratio of 1.2:1.0:1.6:7.2:56.9.
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spelling doaj.art-c409e8a3f5f14fc48e21b95ca4fa89292023-08-29T05:25:38ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192014-10-01841010.56093/ijas.v84i10.44195Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomaceYONGQI YIN0MENGYU LIU1JINXIAN WU2RUNQIANG YANG3ZHENXIN GU4College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China 210 095College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China 210 095College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China 210 095College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China 210 095College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China 210 095 Statistical experimental designs were applied for the optimization of medium constituents for soluble dietary fiber (SDF) production by Ganoderma lucidum in solid state fermentation (SSF) using lotus root pomace (LRP) as the substrate. Factors including wheat bran, MgSO4, FeSO4 and VB1 were identified by Plackett-Burman design (PBD) as significant for SDF production. The path of steepest ascent design (SAD) was undertaken to determine the optimal region of four significant factors. To determine the optimal values of the significant variables, Box-Behnken design (BBD) and response surface analysis were employed. The optimum conditions were found to be the 5g LRP, 1.77 g wheat bran, 0.029 g/L MgSO4, 0.011 g/L FeSO4 and 0.052 g/L VB1. SDF production at these optimum conditions was 0.113 g/g DW substrate. Compositional analysis showed that SDF was composed of rhamnose, xylose, mannose, galactose and glucose in a molar ratio of 1.2:1.0:1.6:7.2:56.9. https://epubs.icar.org.in/index.php/IJAgS/article/view/44195Box-Behnken designGanoderma lucidumLotus root pomacePlackett-Burman designSoluble dietary fiber
spellingShingle YONGQI YIN
MENGYU LIU
JINXIAN WU
RUNQIANG YANG
ZHENXIN GU
Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
The Indian Journal of Agricultural Sciences
Box-Behnken design
Ganoderma lucidum
Lotus root pomace
Plackett-Burman design
Soluble dietary fiber
title Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
title_full Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
title_fullStr Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
title_full_unstemmed Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
title_short Optimization of medium composition for soluble dietary fibre production by Ganoderma lucidum using lotus root pomace
title_sort optimization of medium composition for soluble dietary fibre production by ganoderma lucidum using lotus root pomace
topic Box-Behnken design
Ganoderma lucidum
Lotus root pomace
Plackett-Burman design
Soluble dietary fiber
url https://epubs.icar.org.in/index.php/IJAgS/article/view/44195
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