Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE
In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols....
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Society of Plant Breeders
2015-03-01
|
| Series: | Electronic Journal of Plant Breeding |
| Subjects: | |
| Online Access: | https://sites.google.com/site/ejpb2014/vol-6-1/EJPB_V6_N1_264_268.pdf?attredirects=0&d=1 |
| _version_ | 1828452539602829312 |
|---|---|
| author | Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade |
| author_facet | Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade |
| author_sort | Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade |
| collection | DOAJ |
| description | In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds. |
| first_indexed | 2024-12-10T23:54:23Z |
| format | Article |
| id | doaj.art-c40ab45532d848b59b993eaa910dbdd4 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-10T23:54:23Z |
| publishDate | 2015-03-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-c40ab45532d848b59b993eaa910dbdd42022-12-22T01:28:38ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2015-03-0161264268Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGEAmit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar LangadeIn the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.https://sites.google.com/site/ejpb2014/vol-6-1/EJPB_V6_N1_264_268.pdf?attredirects=0&d=1MaizeGenetic diversitysoluble seed proteinSDS-PAGE |
| spellingShingle | Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE Electronic Journal of Plant Breeding Maize Genetic diversity soluble seed protein SDS-PAGE |
| title | Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE |
| title_full | Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE |
| title_fullStr | Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE |
| title_full_unstemmed | Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE |
| title_short | Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE |
| title_sort | assessment of genetic diversity in maize zea mays l inbred lines on the basis of soluble seed proteins using sds page |
| topic | Maize Genetic diversity soluble seed protein SDS-PAGE |
| url | https://sites.google.com/site/ejpb2014/vol-6-1/EJPB_V6_N1_264_268.pdf?attredirects=0&d=1 |
| work_keys_str_mv | AT amitkumardixitanshumansinghjaiprakashshahiprabhatkumaranddhairyashilmadhukarlangade assessmentofgeneticdiversityinmaizezeamayslinbredlinesonthebasisofsolubleseedproteinsusingsdspage |