YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles
Abstract Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell‐nanoparticle interactions. However, the clinical t...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202302965 |
| _version_ | 1797356427434524672 |
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| author | Marco Cassani Soraia Fernandes Jorge Oliver‐De La Cruz Helena Durikova Jan Vrbsky Marek Patočka Veronika Hegrova Simon Klimovic Jan Pribyl Doriana Debellis Petr Skladal Francesca Cavalieri Frank Caruso Giancarlo Forte |
| author_facet | Marco Cassani Soraia Fernandes Jorge Oliver‐De La Cruz Helena Durikova Jan Vrbsky Marek Patočka Veronika Hegrova Simon Klimovic Jan Pribyl Doriana Debellis Petr Skladal Francesca Cavalieri Frank Caruso Giancarlo Forte |
| author_sort | Marco Cassani |
| collection | DOAJ |
| description | Abstract Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell‐nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell‐nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell‐nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes‐associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple‐negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP‐dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple‐negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy. |
| first_indexed | 2024-03-08T14:26:30Z |
| format | Article |
| id | doaj.art-c4120ba3908f4da5bb68047683a62753 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-08T14:26:30Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-c4120ba3908f4da5bb68047683a627532024-01-13T04:23:06ZengWileyAdvanced Science2198-38442024-01-01112n/an/a10.1002/advs.202302965YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of NanoparticlesMarco Cassani0Soraia Fernandes1Jorge Oliver‐De La Cruz2Helena Durikova3Jan Vrbsky4Marek Patočka5Veronika Hegrova6Simon Klimovic7Jan Pribyl8Doriana Debellis9Petr Skladal10Francesca Cavalieri11Frank Caruso12Giancarlo Forte13International Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicInternational Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicInternational Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicInternational Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicInternational Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicNenoVision Purkynova 649/127 Brno 61200 Czech RepublicNenoVision Purkynova 649/127 Brno 61200 Czech RepublicDepartment of Bioanalytical Instrumentation CEITEC Masaryk University Brno 60200 Czech RepublicDepartment of Bioanalytical Instrumentation CEITEC Masaryk University Brno 60200 Czech RepublicElectron Microscopy Facility Fondazione Istituto Italiano Di Tecnologia Via Morego 30 Genoa 16163 ItalyDepartment of Bioanalytical Instrumentation CEITEC Masaryk University Brno 60200 Czech RepublicDepartment of Chemical Engineering The University of Melbourne Parkville Victoria 3010 AustraliaDepartment of Chemical Engineering The University of Melbourne Parkville Victoria 3010 AustraliaInternational Clinical Research Center St. Anne's University Hospital Brno 60200 Czech RepublicAbstract Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell‐nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell‐nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell‐nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes‐associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple‐negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP‐dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple‐negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy.https://doi.org/10.1002/advs.202302965bio‐nano interactionscancer treatmentmechanobiologynanoparticlesYAP‐signaling |
| spellingShingle | Marco Cassani Soraia Fernandes Jorge Oliver‐De La Cruz Helena Durikova Jan Vrbsky Marek Patočka Veronika Hegrova Simon Klimovic Jan Pribyl Doriana Debellis Petr Skladal Francesca Cavalieri Frank Caruso Giancarlo Forte YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles Advanced Science bio‐nano interactions cancer treatment mechanobiology nanoparticles YAP‐signaling |
| title | YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles |
| title_full | YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles |
| title_fullStr | YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles |
| title_full_unstemmed | YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles |
| title_short | YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles |
| title_sort | yap signaling regulates the cellular uptake and therapeutic effect of nanoparticles |
| topic | bio‐nano interactions cancer treatment mechanobiology nanoparticles YAP‐signaling |
| url | https://doi.org/10.1002/advs.202302965 |
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