Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia
Tsegahun Asfaw,1 Deribew Genetu,2 Demissew Shenkute,1 Tassew Tefera Shenkutie,1 Yosef Eshetie Amare,3 Habtemariam Alekaw Habteweld,4 Berhanu Yitayew1 1Department of Medical Laboratory Science, Debre Berhan University, Debre Berhan, Ethiopia; 2Department of Medical Laboratory Science, Injibara Univer...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-07-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/pathogenic-bacteria-and-their-antibiotic-resistance-patterns-in-milk-y-peer-reviewed-fulltext-article-IDR |
| _version_ | 1827908432042131456 |
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| author | Asfaw T Genetu D Shenkute D Shenkutie TT Amare YE Habteweld HA Yitayew B |
| author_facet | Asfaw T Genetu D Shenkute D Shenkutie TT Amare YE Habteweld HA Yitayew B |
| author_sort | Asfaw T |
| collection | DOAJ |
| description | Tsegahun Asfaw,1 Deribew Genetu,2 Demissew Shenkute,1 Tassew Tefera Shenkutie,1 Yosef Eshetie Amare,3 Habtemariam Alekaw Habteweld,4 Berhanu Yitayew1 1Department of Medical Laboratory Science, Debre Berhan University, Debre Berhan, Ethiopia; 2Department of Medical Laboratory Science, Injibara University, Injibara, Ethiopia; 3Departments of Biomedical Science, Debre Berhan University, Debre Berhan, Ethiopia; 4Departments of Pharmacy, Debre Berhan University, Debre Berhan, EthiopiaCorrespondence: Tsegahun Asfaw, Email tsegahun.asfaw12@gmail.comBackground: Bacterial contamination of milk is a primary culprit for causing foodborne illnesses, presenting a significant health hazard for millions of individuals around the globe. The level and variety of microorganisms present in raw milk determine its degree of contamination and the potential health risks it poses.Methods: A cross-sectional survey was conducted from February to August. A questionnaire was used to collect data on socio-demographic characteristics and hygiene practices from milk distributors and traders. Raw milk, yoghurt, swabs from milk containers and drinking cups were collected and processed for bacterial isolation and identification, antibiotic susceptibility testing, MDR screening and confirmation, ESBL screening and confirmation. Finally, all data were pooled and analyzed using SPSS software version 25.Results: A total of 120 samples of fresh milk, yogurt and cotton swabs from milk containers and cups were collected. A total of 80 bacterial isolates were isolated from 120 samples. Among the bacteria isolated, S. aureus 17 (21.3%), E. coli 17 (21.3%), S. epidermidis 14 (17.5%), Klebsiella spp. 9 (11.3%) and Salmonella spp. 7 (8.8%) were detected most often. High rate of contamination was observed in fresh milk 23 (28.8%) and yogurt 23 (28.8%). All isolates were resistant to at least one antibiotic tested. Comparatively, high rates of resistance were observed in all isolates to the most commonly prescribed antibiotics in Ethiopia. However, lower rates of resistance have been observed for recently introduced antibiotics in Ethiopia. Of the isolates, 20 (25.0%) were resistant to eight or more antibiotics. While 16 (20.0%), 12 (15.0%), 9 (11.3%) isolates were resistant to two, three and five antibiotics, respectively. Of the bacteria isolated, 52/80 (65.0%) were MDR, 25/49 (51.0%) were screened for ESBL production, and 20/49 (40.8%) isolates were confirmed as ESBL producer.Conclusion: This study showed a high rate of bacterial isolates along with MDR and ESBL-producing strains in raw milk, yoghurt, milk container swabs and drinking cup swab samples, associated with poor hygiene and sanitation practices.Keywords: bacterial contamination, multidrug-resistance, extended-spectrum beta-lactamase, raw milk, yoghurt, milk contact surface |
| first_indexed | 2024-03-13T01:22:40Z |
| format | Article |
| id | doaj.art-c418fe7b5b2a443d92d7d658658dde70 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-03-13T01:22:40Z |
| publishDate | 2023-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-c418fe7b5b2a443d92d7d658658dde702023-07-04T19:06:10ZengDove Medical PressInfection and Drug Resistance1178-69732023-07-01Volume 164297430984862Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, EthiopiaAsfaw TGenetu DShenkute DShenkutie TTAmare YEHabteweld HAYitayew BTsegahun Asfaw,1 Deribew Genetu,2 Demissew Shenkute,1 Tassew Tefera Shenkutie,1 Yosef Eshetie Amare,3 Habtemariam Alekaw Habteweld,4 Berhanu Yitayew1 1Department of Medical Laboratory Science, Debre Berhan University, Debre Berhan, Ethiopia; 2Department of Medical Laboratory Science, Injibara University, Injibara, Ethiopia; 3Departments of Biomedical Science, Debre Berhan University, Debre Berhan, Ethiopia; 4Departments of Pharmacy, Debre Berhan University, Debre Berhan, EthiopiaCorrespondence: Tsegahun Asfaw, Email tsegahun.asfaw12@gmail.comBackground: Bacterial contamination of milk is a primary culprit for causing foodborne illnesses, presenting a significant health hazard for millions of individuals around the globe. The level and variety of microorganisms present in raw milk determine its degree of contamination and the potential health risks it poses.Methods: A cross-sectional survey was conducted from February to August. A questionnaire was used to collect data on socio-demographic characteristics and hygiene practices from milk distributors and traders. Raw milk, yoghurt, swabs from milk containers and drinking cups were collected and processed for bacterial isolation and identification, antibiotic susceptibility testing, MDR screening and confirmation, ESBL screening and confirmation. Finally, all data were pooled and analyzed using SPSS software version 25.Results: A total of 120 samples of fresh milk, yogurt and cotton swabs from milk containers and cups were collected. A total of 80 bacterial isolates were isolated from 120 samples. Among the bacteria isolated, S. aureus 17 (21.3%), E. coli 17 (21.3%), S. epidermidis 14 (17.5%), Klebsiella spp. 9 (11.3%) and Salmonella spp. 7 (8.8%) were detected most often. High rate of contamination was observed in fresh milk 23 (28.8%) and yogurt 23 (28.8%). All isolates were resistant to at least one antibiotic tested. Comparatively, high rates of resistance were observed in all isolates to the most commonly prescribed antibiotics in Ethiopia. However, lower rates of resistance have been observed for recently introduced antibiotics in Ethiopia. Of the isolates, 20 (25.0%) were resistant to eight or more antibiotics. While 16 (20.0%), 12 (15.0%), 9 (11.3%) isolates were resistant to two, three and five antibiotics, respectively. Of the bacteria isolated, 52/80 (65.0%) were MDR, 25/49 (51.0%) were screened for ESBL production, and 20/49 (40.8%) isolates were confirmed as ESBL producer.Conclusion: This study showed a high rate of bacterial isolates along with MDR and ESBL-producing strains in raw milk, yoghurt, milk container swabs and drinking cup swab samples, associated with poor hygiene and sanitation practices.Keywords: bacterial contamination, multidrug-resistance, extended-spectrum beta-lactamase, raw milk, yoghurt, milk contact surfacehttps://www.dovepress.com/pathogenic-bacteria-and-their-antibiotic-resistance-patterns-in-milk-y-peer-reviewed-fulltext-article-IDRbacterial contaminationmultidrug-resistanceextended-spectrum beta-lactamaseraw milkyoghurtmilk contact surface. |
| spellingShingle | Asfaw T Genetu D Shenkute D Shenkutie TT Amare YE Habteweld HA Yitayew B Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia Infection and Drug Resistance bacterial contamination multidrug-resistance extended-spectrum beta-lactamase raw milk yoghurt milk contact surface. |
| title | Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia |
| title_full | Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia |
| title_fullStr | Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia |
| title_full_unstemmed | Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia |
| title_short | Pathogenic Bacteria and Their Antibiotic Resistance Patterns in Milk, Yoghurt and Milk Contact Surfaces in Debre Berhan Town, Ethiopia |
| title_sort | pathogenic bacteria and their antibiotic resistance patterns in milk yoghurt and milk contact surfaces in debre berhan town ethiopia |
| topic | bacterial contamination multidrug-resistance extended-spectrum beta-lactamase raw milk yoghurt milk contact surface. |
| url | https://www.dovepress.com/pathogenic-bacteria-and-their-antibiotic-resistance-patterns-in-milk-y-peer-reviewed-fulltext-article-IDR |
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