Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola
Clubroot disease resistance has been introgressed from <i>Brassica rapa</i> into canola following different approaches including a <i>B. napus</i> × <i>B. rapa</i> interspecific cross; however, the details of this cross are not available. To extend our knowledge o...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/5/1225 |
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| author | Kawalpreet Kaur Yingyi Liu Habibur Rahman |
| author_facet | Kawalpreet Kaur Yingyi Liu Habibur Rahman |
| author_sort | Kawalpreet Kaur |
| collection | DOAJ |
| description | Clubroot disease resistance has been introgressed from <i>Brassica rapa</i> into canola following different approaches including a <i>B. napus</i> × <i>B. rapa</i> interspecific cross; however, the details of this cross are not available. To extend our knowledge of this cross for the introgression of resistance to multiple pathotypes, we crossed a clubroot-resistant turnip with a susceptible canola and backcrossed the F<sub>1</sub>′s to canola. The backcross population was subjected to pedigree breeding with selection for clubroot resistance and canola-quality traits; selection for resistance to pathotype 3H and 3A was performed in the early and advanced generations, respectively. The advanced generation lines were also evaluated for resistance to 3H, 3A, 2B, and 5x, and for field resistance to clubroot, and agronomic and seed quality traits. Following this, we developed canola lines carrying resistance to multiple pathotypes and nuclear DNA content similar to <i>B. napus</i>. Resistance to 3H showed a weak correlation with other pathotypes whereas resistance to 3A showed a strong positive correlation with 5x indicating that resistance to these pathotypes was under a similar genetic control. Thus, the results demonstrate that resistance to multiple pathotypes can be introgressed from a turnip into a canola even when selection for resistance to single pathotype is performed in early generations. |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:29:39Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c419473ba53843cebeab73a5c6d39d242023-11-23T09:44:46ZengMDPI AGAgronomy2073-43952022-05-01125122510.3390/agronomy12051225Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> CanolaKawalpreet Kaur0Yingyi Liu1Habibur Rahman2Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, CanadaClubroot disease resistance has been introgressed from <i>Brassica rapa</i> into canola following different approaches including a <i>B. napus</i> × <i>B. rapa</i> interspecific cross; however, the details of this cross are not available. To extend our knowledge of this cross for the introgression of resistance to multiple pathotypes, we crossed a clubroot-resistant turnip with a susceptible canola and backcrossed the F<sub>1</sub>′s to canola. The backcross population was subjected to pedigree breeding with selection for clubroot resistance and canola-quality traits; selection for resistance to pathotype 3H and 3A was performed in the early and advanced generations, respectively. The advanced generation lines were also evaluated for resistance to 3H, 3A, 2B, and 5x, and for field resistance to clubroot, and agronomic and seed quality traits. Following this, we developed canola lines carrying resistance to multiple pathotypes and nuclear DNA content similar to <i>B. napus</i>. Resistance to 3H showed a weak correlation with other pathotypes whereas resistance to 3A showed a strong positive correlation with 5x indicating that resistance to these pathotypes was under a similar genetic control. Thus, the results demonstrate that resistance to multiple pathotypes can be introgressed from a turnip into a canola even when selection for resistance to single pathotype is performed in early generations.https://www.mdpi.com/2073-4395/12/5/1225canolaturnip<i>Plasmodiophora brassicae</i>multiple pathotypesintrogressioninterspecific cross |
| spellingShingle | Kawalpreet Kaur Yingyi Liu Habibur Rahman Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola Agronomy canola turnip <i>Plasmodiophora brassicae</i> multiple pathotypes introgression interspecific cross |
| title | Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola |
| title_full | Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola |
| title_fullStr | Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola |
| title_full_unstemmed | Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola |
| title_short | Introgression of Resistance to Multiple Pathotypes of <i>Plasmodiophora brassicae</i> from Turnip (<i>Brassica rapa</i> ssp. <i>rapifera</i>) into Spring <i>B. napus</i> Canola |
| title_sort | introgression of resistance to multiple pathotypes of i plasmodiophora brassicae i from turnip i brassica rapa i ssp i rapifera i into spring i b napus i canola |
| topic | canola turnip <i>Plasmodiophora brassicae</i> multiple pathotypes introgression interspecific cross |
| url | https://www.mdpi.com/2073-4395/12/5/1225 |
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