The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant
Haiyan Wang, 1 Qiuping Xu, 1 Hui Hu, 2 Chunling Shi, 3 Ziyan Lin, 3 Huixi Jiang, 3 Huaipu Dong, 3 Jing Guo 1 1Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shan...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2020-11-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-fabrication-and-function-of-strontium-modified-hierarchical-micron-peer-reviewed-article-IJN |
| _version_ | 1818990944169492480 |
|---|---|
| author | Wang H Xu Q Hu H Shi C Lin Z Jiang H Dong H Guo J |
| author_facet | Wang H Xu Q Hu H Shi C Lin Z Jiang H Dong H Guo J |
| author_sort | Wang H |
| collection | DOAJ |
| description | Haiyan Wang, 1 Qiuping Xu, 1 Hui Hu, 2 Chunling Shi, 3 Ziyan Lin, 3 Huixi Jiang, 3 Huaipu Dong, 3 Jing Guo 1 1Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, 250012, People’s Republic of China; 2Osaka Dental University Kusuha School, Hirakata City, Osaka 573-1121, Japan; 3School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, People’s Republic of ChinaCorrespondence: Jing GuoDepartment of Orthodontics, Room 201, School and Hospital of Stomatology, Shandong University, 44-1 Wenhua West Road, Jinan, Shandong 250012, Mainland ChinaEmail guojing@sdu.edu.cnBackground: Relying on surface topography alone to enhance the osteointegration of implants is still inadequate. An effective way to combine long-term ion release and surface topography to enhance osteogenic property is urgently needed.Purpose: The objective of this study is to fabricate a long-term strontium ion release implant system and confirm the biological function in vitro and in vivo.Methods: The biomimic surface was fabricated through alkali-heat treatment and magnetron sputtering. The in vitro biological function assays were determined by MTT, fluorescence staining, alkaline phosphatase activity, extracellular mineralization, and quantitative real-time polymerase chain reaction assays. The in vivo experiments were detected by micro-CT, HE staining and Masson staining.Results: The biomimic surface structure has been successfully fabricated. The in vitro cell assays determined that AH-Ti/Sr90 possessed the best biological function. The in vivo experiments demonstrated that AH-Ti/Sr90 could promote osteointegration significantly under both in normal and osteoporotic conditions.Conclusion: We determined that AH-Ti/Sr90 possesses the best osteogenic property, long-term ion release capacity and osteointegration promotion ability. It has potential clinic application prospects.Keywords: titanium, alkali-heat treatment, magnetron sputtering, osteogenic differentiation, osteoclast inhibition |
| first_indexed | 2024-12-20T20:02:25Z |
| format | Article |
| id | doaj.art-c41b8d0de50d487ba1f9ae9c9d28f4aa |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-20T20:02:25Z |
| publishDate | 2020-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-c41b8d0de50d487ba1f9ae9c9d28f4aa2022-12-21T19:28:02ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-11-01Volume 158983899859324The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium ImplantWang HXu QHu HShi CLin ZJiang HDong HGuo JHaiyan Wang, 1 Qiuping Xu, 1 Hui Hu, 2 Chunling Shi, 3 Ziyan Lin, 3 Huixi Jiang, 3 Huaipu Dong, 3 Jing Guo 1 1Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, 250012, People’s Republic of China; 2Osaka Dental University Kusuha School, Hirakata City, Osaka 573-1121, Japan; 3School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, 325027, People’s Republic of ChinaCorrespondence: Jing GuoDepartment of Orthodontics, Room 201, School and Hospital of Stomatology, Shandong University, 44-1 Wenhua West Road, Jinan, Shandong 250012, Mainland ChinaEmail guojing@sdu.edu.cnBackground: Relying on surface topography alone to enhance the osteointegration of implants is still inadequate. An effective way to combine long-term ion release and surface topography to enhance osteogenic property is urgently needed.Purpose: The objective of this study is to fabricate a long-term strontium ion release implant system and confirm the biological function in vitro and in vivo.Methods: The biomimic surface was fabricated through alkali-heat treatment and magnetron sputtering. The in vitro biological function assays were determined by MTT, fluorescence staining, alkaline phosphatase activity, extracellular mineralization, and quantitative real-time polymerase chain reaction assays. The in vivo experiments were detected by micro-CT, HE staining and Masson staining.Results: The biomimic surface structure has been successfully fabricated. The in vitro cell assays determined that AH-Ti/Sr90 possessed the best biological function. The in vivo experiments demonstrated that AH-Ti/Sr90 could promote osteointegration significantly under both in normal and osteoporotic conditions.Conclusion: We determined that AH-Ti/Sr90 possesses the best osteogenic property, long-term ion release capacity and osteointegration promotion ability. It has potential clinic application prospects.Keywords: titanium, alkali-heat treatment, magnetron sputtering, osteogenic differentiation, osteoclast inhibitionhttps://www.dovepress.com/the-fabrication-and-function-of-strontium-modified-hierarchical-micron-peer-reviewed-article-IJNtitaniumalkali-heat treatmentmagnetron sputteringosteogenic differentiationosteoclast inhibition |
| spellingShingle | Wang H Xu Q Hu H Shi C Lin Z Jiang H Dong H Guo J The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant International Journal of Nanomedicine titanium alkali-heat treatment magnetron sputtering osteogenic differentiation osteoclast inhibition |
| title | The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant |
| title_full | The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant |
| title_fullStr | The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant |
| title_full_unstemmed | The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant |
| title_short | The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant |
| title_sort | fabrication and function of strontium modified hierarchical micro nano titanium implant |
| topic | titanium alkali-heat treatment magnetron sputtering osteogenic differentiation osteoclast inhibition |
| url | https://www.dovepress.com/the-fabrication-and-function-of-strontium-modified-hierarchical-micron-peer-reviewed-article-IJN |
| work_keys_str_mv | AT wangh thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT xuq thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT huh thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT shic thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT linz thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT jiangh thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT dongh thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT guoj thefabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT wangh fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT xuq fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT huh fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT shic fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT linz fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT jiangh fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT dongh fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant AT guoj fabricationandfunctionofstrontiummodifiedhierarchicalmicronanotitaniumimplant |