Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose
<p>Insulin-like growth factor 1 (<i>IGF1</i>) is one of the endocrine hormones that plays an important role in regulating growth and development of animals. In this study, polymorphism in the 5<span class="inline-formula"><sup>′</sup></span>UTR and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2021-07-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/64/315/2021/aab-64-315-2021.pdf |
| _version_ | 1830111540135591936 |
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| author | J. Tang J. Tang M. Guo J. Fu H. Ouyang H. Ouyang Y. Tian Y. Tian X. Shen X. Shen Y. Huang Y. Huang |
| author_facet | J. Tang J. Tang M. Guo J. Fu H. Ouyang H. Ouyang Y. Tian Y. Tian X. Shen X. Shen Y. Huang Y. Huang |
| author_sort | J. Tang |
| collection | DOAJ |
| description | <p>Insulin-like growth factor 1 (<i>IGF1</i>) is one of the
endocrine hormones that plays an important role in regulating
growth and development of animals. In this study, polymorphism in the 5<span class="inline-formula"><sup>′</sup></span>UTR
and 3<span class="inline-formula"><sup>′</sup></span>UTR coding region and of the <i>IGF1</i> gene was detected by DNA sequencing
technology, and the abundance of <i>IGF1</i> mRNA in various tissues at three growth
stages of the Shitou goose was determined by quantitative real-time polymerase chain reaction
(qRT-PCR). Moreover, the differential expression of <i>IGF1</i> in various tissues
between the Shitou goose and Wuzong goose was revealed. Two single nucleotide
polymorphisms (SNPs) were found in the exon3 region of <i>IGF1</i> in the Shitou goose. <i>IGF1</i> mRNA
was extensively expressed in various tissues of Shitou geese with high
abundant expression in the liver, breast muscle and leg muscle at three growth
stages. <i>IGF1</i> mRNA expression showed a trend of first increase and then decrease
in the pituitary, liver, subcutaneous fat and abdominal fat tissues, but it
decreased in the breast muscle and leg muscle of a Shitou goose with growing age.
Expression of <i>IGF1</i> in the liver, leg muscle and pituitary tissues of the Shitou goose
was significantly higher than that of the Wuzong goose. This provides a
foundation for further study of regulatory mechanisms of <i>IGF1</i> in the growth and
development of geese.</p> |
| first_indexed | 2024-12-16T23:54:54Z |
| format | Article |
| id | doaj.art-c41d81fc22b845c787e1faf847faafe8 |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-12-16T23:54:54Z |
| publishDate | 2021-07-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-c41d81fc22b845c787e1faf847faafe82022-12-21T22:11:14ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222021-07-016431532310.5194/aab-64-315-2021Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou gooseJ. Tang0J. Tang1M. Guo2J. Fu3H. Ouyang4H. Ouyang5Y. Tian6Y. Tian7X. Shen8X. Shen9Y. Huang10Y. Huang11College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaGuangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaGuangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaGuangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaGuangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. ChinaCollege of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. ChinaGuangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. China<p>Insulin-like growth factor 1 (<i>IGF1</i>) is one of the endocrine hormones that plays an important role in regulating growth and development of animals. In this study, polymorphism in the 5<span class="inline-formula"><sup>′</sup></span>UTR and 3<span class="inline-formula"><sup>′</sup></span>UTR coding region and of the <i>IGF1</i> gene was detected by DNA sequencing technology, and the abundance of <i>IGF1</i> mRNA in various tissues at three growth stages of the Shitou goose was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the differential expression of <i>IGF1</i> in various tissues between the Shitou goose and Wuzong goose was revealed. Two single nucleotide polymorphisms (SNPs) were found in the exon3 region of <i>IGF1</i> in the Shitou goose. <i>IGF1</i> mRNA was extensively expressed in various tissues of Shitou geese with high abundant expression in the liver, breast muscle and leg muscle at three growth stages. <i>IGF1</i> mRNA expression showed a trend of first increase and then decrease in the pituitary, liver, subcutaneous fat and abdominal fat tissues, but it decreased in the breast muscle and leg muscle of a Shitou goose with growing age. Expression of <i>IGF1</i> in the liver, leg muscle and pituitary tissues of the Shitou goose was significantly higher than that of the Wuzong goose. This provides a foundation for further study of regulatory mechanisms of <i>IGF1</i> in the growth and development of geese.</p>https://aab.copernicus.org/articles/64/315/2021/aab-64-315-2021.pdf |
| spellingShingle | J. Tang J. Tang M. Guo J. Fu H. Ouyang H. Ouyang Y. Tian Y. Tian X. Shen X. Shen Y. Huang Y. Huang Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose Archives Animal Breeding |
| title | Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose |
| title_full | Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose |
| title_fullStr | Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose |
| title_full_unstemmed | Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose |
| title_short | Polymorphism analysis and expression patterns of the <i>IGF1</i> gene in the Shitou goose |
| title_sort | polymorphism analysis and expression patterns of the i igf1 i gene in the shitou goose |
| url | https://aab.copernicus.org/articles/64/315/2021/aab-64-315-2021.pdf |
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