A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain
The distinct organization of the brain’s vasculature ensures the adequate delivery of oxygen and nutrients during development and adulthood. Acute and chronic pathological changes of the vascular system have been implicated in many neurological disorders including stroke and dementia. Here, we descr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2020.00244/full |
| _version_ | 1818446555618738176 |
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| author | Ruslan Rust Ruslan Rust Ruslan Rust Tunahan Kirabali Tunahan Kirabali Lisa Grönnert Berre Dogancay Yanuar D. P. Limasale Andrea Meinhardt Carsten Werner Bàrbara Laviña Luka Kulic Luka Kulic Roger M. Nitsch Roger M. Nitsch Christian Tackenberg Christian Tackenberg Martin E. Schwab Martin E. Schwab Martin E. Schwab |
| author_facet | Ruslan Rust Ruslan Rust Ruslan Rust Tunahan Kirabali Tunahan Kirabali Lisa Grönnert Berre Dogancay Yanuar D. P. Limasale Andrea Meinhardt Carsten Werner Bàrbara Laviña Luka Kulic Luka Kulic Roger M. Nitsch Roger M. Nitsch Christian Tackenberg Christian Tackenberg Martin E. Schwab Martin E. Schwab Martin E. Schwab |
| author_sort | Ruslan Rust |
| collection | DOAJ |
| description | The distinct organization of the brain’s vasculature ensures the adequate delivery of oxygen and nutrients during development and adulthood. Acute and chronic pathological changes of the vascular system have been implicated in many neurological disorders including stroke and dementia. Here, we describe a fast, automated method that allows the highly reproducible, quantitative assessment of distinct vascular parameters and their changes based on the open source software Fiji (ImageJ). In particular, we developed a practical guide to reliably measure aspects of growth, repair and maturation of the brain’s vasculature during development and neurovascular disease in mice and humans. The script can be used to assess the effects of different external factors including pharmacological treatments or disease states. Moreover, the procedure is expandable to blood vessels of other organs and vascular in vitro models. |
| first_indexed | 2024-12-14T19:49:35Z |
| format | Article |
| id | doaj.art-c41fbfe134e64b1f856f20b02966e067 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-14T19:49:35Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-c41fbfe134e64b1f856f20b02966e0672022-12-21T22:49:28ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2020-03-011410.3389/fnins.2020.00244511979A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the BrainRuslan Rust0Ruslan Rust1Ruslan Rust2Tunahan Kirabali3Tunahan Kirabali4Lisa Grönnert5Berre Dogancay6Yanuar D. P. Limasale7Andrea Meinhardt8Carsten Werner9Bàrbara Laviña10Luka Kulic11Luka Kulic12Roger M. Nitsch13Roger M. Nitsch14Christian Tackenberg15Christian Tackenberg16Martin E. Schwab17Martin E. Schwab18Martin E. Schwab19Institute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandDepartment of Health Sciences and Technology, ETH Zürich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandLeibniz Institute for Polymer Research, Dresden, GermanyLeibniz Institute for Polymer Research, Dresden, GermanyLeibniz Institute for Polymer Research, Dresden, GermanyDepartment of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SwedenInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandInstitute for Regenerative Medicine, University of Zurich, Zurich, SwitzerlandDepartment of Health Sciences and Technology, ETH Zürich, Zurich, SwitzerlandNeuroscience Center Zurich, University of Zurich and ETH Zürich, Zurich, SwitzerlandThe distinct organization of the brain’s vasculature ensures the adequate delivery of oxygen and nutrients during development and adulthood. Acute and chronic pathological changes of the vascular system have been implicated in many neurological disorders including stroke and dementia. Here, we describe a fast, automated method that allows the highly reproducible, quantitative assessment of distinct vascular parameters and their changes based on the open source software Fiji (ImageJ). In particular, we developed a practical guide to reliably measure aspects of growth, repair and maturation of the brain’s vasculature during development and neurovascular disease in mice and humans. The script can be used to assess the effects of different external factors including pharmacological treatments or disease states. Moreover, the procedure is expandable to blood vessels of other organs and vascular in vitro models.https://www.frontiersin.org/article/10.3389/fnins.2020.00244/fullquantificationimage processingangiogenesisblood vesselsstrokedevelopment |
| spellingShingle | Ruslan Rust Ruslan Rust Ruslan Rust Tunahan Kirabali Tunahan Kirabali Lisa Grönnert Berre Dogancay Yanuar D. P. Limasale Andrea Meinhardt Carsten Werner Bàrbara Laviña Luka Kulic Luka Kulic Roger M. Nitsch Roger M. Nitsch Christian Tackenberg Christian Tackenberg Martin E. Schwab Martin E. Schwab Martin E. Schwab A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain Frontiers in Neuroscience quantification image processing angiogenesis blood vessels stroke development |
| title | A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain |
| title_full | A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain |
| title_fullStr | A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain |
| title_full_unstemmed | A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain |
| title_short | A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain |
| title_sort | practical guide to the automated analysis of vascular growth maturation and injury in the brain |
| topic | quantification image processing angiogenesis blood vessels stroke development |
| url | https://www.frontiersin.org/article/10.3389/fnins.2020.00244/full |
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