Kinetochore-independent chromosome segregation driven by lateral microtubule bundles
During cell division, chromosomes attach to spindle microtubules at sites called kinetochores, and force generated at the kinetochore-microtubule interface is the main driver of chromosome movement. Surprisingly, kinetochores are not required for chromosome segregation on acentrosomal spindles in Ca...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2015-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/06462 |
| _version_ | 1828107611718811648 |
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| author | Christina C Muscat Keila M Torre-Santiago Michael V Tran James A Powers Sarah M Wignall |
| author_facet | Christina C Muscat Keila M Torre-Santiago Michael V Tran James A Powers Sarah M Wignall |
| author_sort | Christina C Muscat |
| collection | DOAJ |
| description | During cell division, chromosomes attach to spindle microtubules at sites called kinetochores, and force generated at the kinetochore-microtubule interface is the main driver of chromosome movement. Surprisingly, kinetochores are not required for chromosome segregation on acentrosomal spindles in Caenorhabditis elegans oocytes, but the mechanism driving chromosomes apart in their absence is not understood. In this study, we show that lateral microtubule–chromosome associations established during prometaphase remain intact during anaphase to facilitate separation, defining a novel form of kinetochore-independent segregation. Chromosome dynamics during congression and segregation are controlled by opposing forces; plus-end directed forces are mediated by a protein complex that forms a ring around the chromosome center and dynein on chromosome arms provides a minus-end force. At anaphase onset, ring removal shifts the balance between these forces, triggering poleward movement along lateral microtubule bundles. This represents an elegant strategy for controlling chromosomal movements during cell division distinct from the canonical kinetochore-driven mechanism. |
| first_indexed | 2024-04-11T10:33:57Z |
| format | Article |
| id | doaj.art-c4229fd7714e4e4dbb40a5ab82e96560 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:33:57Z |
| publishDate | 2015-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c4229fd7714e4e4dbb40a5ab82e965602022-12-22T04:29:21ZengeLife Sciences Publications LtdeLife2050-084X2015-05-01410.7554/eLife.06462Kinetochore-independent chromosome segregation driven by lateral microtubule bundlesChristina C Muscat0Keila M Torre-Santiago1Michael V Tran2James A Powers3Sarah M Wignall4Department of Molecular Biosciences, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesLight Microscopy Imaging Center, Indiana University, Bloomington, United StatesDepartment of Molecular Biosciences, Northwestern University, Evanston, United StatesDuring cell division, chromosomes attach to spindle microtubules at sites called kinetochores, and force generated at the kinetochore-microtubule interface is the main driver of chromosome movement. Surprisingly, kinetochores are not required for chromosome segregation on acentrosomal spindles in Caenorhabditis elegans oocytes, but the mechanism driving chromosomes apart in their absence is not understood. In this study, we show that lateral microtubule–chromosome associations established during prometaphase remain intact during anaphase to facilitate separation, defining a novel form of kinetochore-independent segregation. Chromosome dynamics during congression and segregation are controlled by opposing forces; plus-end directed forces are mediated by a protein complex that forms a ring around the chromosome center and dynein on chromosome arms provides a minus-end force. At anaphase onset, ring removal shifts the balance between these forces, triggering poleward movement along lateral microtubule bundles. This represents an elegant strategy for controlling chromosomal movements during cell division distinct from the canonical kinetochore-driven mechanism.https://elifesciences.org/articles/06462mitosismeiosisoocytespindlemicrotubulecytoskeleton |
| spellingShingle | Christina C Muscat Keila M Torre-Santiago Michael V Tran James A Powers Sarah M Wignall Kinetochore-independent chromosome segregation driven by lateral microtubule bundles eLife mitosis meiosis oocyte spindle microtubule cytoskeleton |
| title | Kinetochore-independent chromosome segregation driven by lateral microtubule bundles |
| title_full | Kinetochore-independent chromosome segregation driven by lateral microtubule bundles |
| title_fullStr | Kinetochore-independent chromosome segregation driven by lateral microtubule bundles |
| title_full_unstemmed | Kinetochore-independent chromosome segregation driven by lateral microtubule bundles |
| title_short | Kinetochore-independent chromosome segregation driven by lateral microtubule bundles |
| title_sort | kinetochore independent chromosome segregation driven by lateral microtubule bundles |
| topic | mitosis meiosis oocyte spindle microtubule cytoskeleton |
| url | https://elifesciences.org/articles/06462 |
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