A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis
ABSTRACT Recent evidence emphasized the role of the microbiota in the etiopathogenesis of rheumatoid arthritis (RA). Indeed, it has been demonstrated that urinary tract infections are implicated in RA pathogenesis. However, a definitive association between the urinary tract microbiota and RA remains...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-06-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03662-22 |
| _version_ | 1797803487862456320 |
|---|---|
| author | Jiayang Jin Jing Li Meiling Hou Xu Ding Yan Zhong Jing He Xiaolin Sun Hua Ye Ru Li Lijun Wu Jun Wang Jianping Guo Zhanguo Li |
| author_facet | Jiayang Jin Jing Li Meiling Hou Xu Ding Yan Zhong Jing He Xiaolin Sun Hua Ye Ru Li Lijun Wu Jun Wang Jianping Guo Zhanguo Li |
| author_sort | Jiayang Jin |
| collection | DOAJ |
| description | ABSTRACT Recent evidence emphasized the role of the microbiota in the etiopathogenesis of rheumatoid arthritis (RA). Indeed, it has been demonstrated that urinary tract infections are implicated in RA pathogenesis. However, a definitive association between the urinary tract microbiota and RA remains to be investigated. Urine samples from 39 patients affected by RA, including treatment-naive patients, and 37 age- and sex-matched healthy individuals were collected. In RA patients, the urinary microbiota showed an increase in microbial richness and a decrease in microbial dissimilarity, especially in treatment-naive patients. A total of 48 altered genera with different absolute quantities were detected in patients with RA. The 37 enriched genera included Proteus, Faecalibacterium, and Bacteroides, while the 11 deficient genera included Gardnerella, Ruminococcus, Megasphaera, and Ureaplasma. Notably, the more abundant genera in RA patients were correlated with the disease activity score of 28 joints-erythrocyte sedimentation rates (DAS28-ESR) and an increase in plasma B cells. Furthermore, the altered urinary metabolites, such as proline, citric acid, and oxalic acid, were positively associated with RA patients, and they were closely correlated with urinary microbiota. These findings suggested a strong association between the altered urinary microbiota and metabolites with disease severity and dysregulated immune responses in RA patients. IMPORTANCE We revealed that the profile of the urinary tract microbiota in RA featured with increased microbial richness and shifted taxa, associated with immunological and metabolic changes of the disease, underlining the interplay between urinary microbiota and host autoimmunity. |
| first_indexed | 2024-03-13T05:21:39Z |
| format | Article |
| id | doaj.art-c4236c905e2c4a21be2b3fb95228b801 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:21:39Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-c4236c905e2c4a21be2b3fb95228b8012023-06-15T13:18:30ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.03662-22A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid ArthritisJiayang Jin0Jing Li1Meiling Hou2Xu Ding3Yan Zhong4Jing He5Xiaolin Sun6Hua Ye7Ru Li8Lijun Wu9Jun Wang10Jianping Guo11Zhanguo Li12Department of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaTinyGene Bio-Tech (Shanghai) Co., Ltd., Shanghai, ChinaTinyGene Bio-Tech (Shanghai) Co., Ltd., Shanghai, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, The People’s Hospital of Xin Jiang Uygur Autonomous Region, Urumqi, ChinaCAS Key Laboratory for Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing, ChinaABSTRACT Recent evidence emphasized the role of the microbiota in the etiopathogenesis of rheumatoid arthritis (RA). Indeed, it has been demonstrated that urinary tract infections are implicated in RA pathogenesis. However, a definitive association between the urinary tract microbiota and RA remains to be investigated. Urine samples from 39 patients affected by RA, including treatment-naive patients, and 37 age- and sex-matched healthy individuals were collected. In RA patients, the urinary microbiota showed an increase in microbial richness and a decrease in microbial dissimilarity, especially in treatment-naive patients. A total of 48 altered genera with different absolute quantities were detected in patients with RA. The 37 enriched genera included Proteus, Faecalibacterium, and Bacteroides, while the 11 deficient genera included Gardnerella, Ruminococcus, Megasphaera, and Ureaplasma. Notably, the more abundant genera in RA patients were correlated with the disease activity score of 28 joints-erythrocyte sedimentation rates (DAS28-ESR) and an increase in plasma B cells. Furthermore, the altered urinary metabolites, such as proline, citric acid, and oxalic acid, were positively associated with RA patients, and they were closely correlated with urinary microbiota. These findings suggested a strong association between the altered urinary microbiota and metabolites with disease severity and dysregulated immune responses in RA patients. IMPORTANCE We revealed that the profile of the urinary tract microbiota in RA featured with increased microbial richness and shifted taxa, associated with immunological and metabolic changes of the disease, underlining the interplay between urinary microbiota and host autoimmunity.https://journals.asm.org/doi/10.1128/spectrum.03662-22rheumatoid arthritisurinary microbiotaurinary metabolitesimmune responses |
| spellingShingle | Jiayang Jin Jing Li Meiling Hou Xu Ding Yan Zhong Jing He Xiaolin Sun Hua Ye Ru Li Lijun Wu Jun Wang Jianping Guo Zhanguo Li A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis Microbiology Spectrum rheumatoid arthritis urinary microbiota urinary metabolites immune responses |
| title | A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis |
| title_full | A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis |
| title_fullStr | A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis |
| title_full_unstemmed | A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis |
| title_short | A Shifted Urinary Microbiota Associated with Disease Activity and Immune Responses in Rheumatoid Arthritis |
| title_sort | shifted urinary microbiota associated with disease activity and immune responses in rheumatoid arthritis |
| topic | rheumatoid arthritis urinary microbiota urinary metabolites immune responses |
| url | https://journals.asm.org/doi/10.1128/spectrum.03662-22 |
| work_keys_str_mv | AT jiayangjin ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jingli ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT meilinghou ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT xuding ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT yanzhong ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jinghe ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT xiaolinsun ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT huaye ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT ruli ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT lijunwu ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT junwang ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jianpingguo ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT zhanguoli ashiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jiayangjin shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jingli shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT meilinghou shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT xuding shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT yanzhong shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jinghe shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT xiaolinsun shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT huaye shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT ruli shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT lijunwu shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT junwang shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT jianpingguo shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis AT zhanguoli shiftedurinarymicrobiotaassociatedwithdiseaseactivityandimmuneresponsesinrheumatoidarthritis |