Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles
<p>Abstract</p> <p>Background</p> <p>This paper examines the mechanism of ultrasonic enhanced drug delivery from Pluronic micelles. In previous publications by our group, fluorescently labeled Pluronic was shown to penetrate HL-60 cells with and without the action of ul...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2002-08-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2407/2/20 |
| _version_ | 1828747391958777856 |
|---|---|
| author | Runyan Christopher M Husseini Ghaleb A Pitt William G |
| author_facet | Runyan Christopher M Husseini Ghaleb A Pitt William G |
| author_sort | Runyan Christopher M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>This paper examines the mechanism of ultrasonic enhanced drug delivery from Pluronic micelles. In previous publications by our group, fluorescently labeled Pluronic was shown to penetrate HL-60 cells with and without the action of ultrasound, while drug uptake was increased with the application of ultrasound.</p> <p>Methods</p> <p>In this study, the amount of uptake of two fluorescent probes, Lysosensor Green (a pH-sensitive probe) and Cell Tracker Orange CMTMR (a pH-independent probe), was measured in HL-60 and HeLa cells.</p> <p>Results</p> <p>The results of our experiments show that the increase in drug accumulation in the cells as a result of ultrasonication is not due to an increase in endocytosis due to ultrasonication.</p> <p>Conclusions</p> <p>We hypothesize that sonoporation plays an important role in the acoustically activated drug delivery of chemotherapy drugs delivered from Pluronic micelles.</p> |
| first_indexed | 2024-04-14T04:41:13Z |
| format | Article |
| id | doaj.art-c423aa607b75417aba085c4f5a92b134 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-14T04:41:13Z |
| publishDate | 2002-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-c423aa607b75417aba085c4f5a92b1342022-12-22T02:11:40ZengBMCBMC Cancer1471-24072002-08-01212010.1186/1471-2407-2-20Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micellesRunyan Christopher MHusseini Ghaleb APitt William G<p>Abstract</p> <p>Background</p> <p>This paper examines the mechanism of ultrasonic enhanced drug delivery from Pluronic micelles. In previous publications by our group, fluorescently labeled Pluronic was shown to penetrate HL-60 cells with and without the action of ultrasound, while drug uptake was increased with the application of ultrasound.</p> <p>Methods</p> <p>In this study, the amount of uptake of two fluorescent probes, Lysosensor Green (a pH-sensitive probe) and Cell Tracker Orange CMTMR (a pH-independent probe), was measured in HL-60 and HeLa cells.</p> <p>Results</p> <p>The results of our experiments show that the increase in drug accumulation in the cells as a result of ultrasonication is not due to an increase in endocytosis due to ultrasonication.</p> <p>Conclusions</p> <p>We hypothesize that sonoporation plays an important role in the acoustically activated drug delivery of chemotherapy drugs delivered from Pluronic micelles.</p>http://www.biomedcentral.com/1471-2407/2/20Micellar drug deliveryPluronicflow cytometryultrasound |
| spellingShingle | Runyan Christopher M Husseini Ghaleb A Pitt William G Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles BMC Cancer Micellar drug delivery Pluronic flow cytometry ultrasound |
| title | Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles |
| title_full | Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles |
| title_fullStr | Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles |
| title_full_unstemmed | Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles |
| title_short | Investigating the mechanism of acoustically activated uptake of drugs from Pluronic micelles |
| title_sort | investigating the mechanism of acoustically activated uptake of drugs from pluronic micelles |
| topic | Micellar drug delivery Pluronic flow cytometry ultrasound |
| url | http://www.biomedcentral.com/1471-2407/2/20 |
| work_keys_str_mv | AT runyanchristopherm investigatingthemechanismofacousticallyactivateduptakeofdrugsfrompluronicmicelles AT husseinighaleba investigatingthemechanismofacousticallyactivateduptakeofdrugsfrompluronicmicelles AT pittwilliamg investigatingthemechanismofacousticallyactivateduptakeofdrugsfrompluronicmicelles |