Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition
IntroductionSymbiotic N fixation inhibition induced by N supply to legumes is potentially regulated by the relative N and P availability in soil. However, the specific responses of different legume species to changes in N:P availability remain unclear, and must be better understood to optimize symbi...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1076894/full |
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| author | Qiang Li Qiang Li Joshua Philp Matthew D. Denton Yingxin Huang Yingxin Huang Jian Wei Huijuan Sun Huijuan Sun Yang Li Yang Li Qian Zhao |
| author_facet | Qiang Li Qiang Li Joshua Philp Matthew D. Denton Yingxin Huang Yingxin Huang Jian Wei Huijuan Sun Huijuan Sun Yang Li Yang Li Qian Zhao |
| author_sort | Qiang Li |
| collection | DOAJ |
| description | IntroductionSymbiotic N fixation inhibition induced by N supply to legumes is potentially regulated by the relative N and P availability in soil. However, the specific responses of different legume species to changes in N:P availability remain unclear, and must be better understood to optimize symbiotic N fixation inputs under N enrichment. This study investigated mechanisms by which soil N and P supply influence the symbiotic N fixation of eight legume species, to quantify the inter-specific differences, and to demonstrate how these differences can be determined by the stoichiometric homeostasis in N:P ratios (HN:P).MethodsEight herbaceous legume species were grown separately in outdoor pots and treated with either no fertilizer (control), N fertilizer (14 g N m-2), P fertilizer (3.5 g P m-2) or both N and P fertilizer. Plant nutrients, stoichiometric characteristics, root biomass, non-structural carbohydrates (NSC), rhizosphere chemistry, P mobilization, root nodulation and symbiotic N fixation were measured.ResultsN addition enhanced rhizosphere P mobilization but drove a loss of root biomass and root NSC via exudation of P mobilization compound (organic acid), especially so in treatments without P addition. N addition also induced a 2-14% or 14-36% decline in symbiotic N fixation per plant biomass by legumes in treatments with or without P addition, as a result of decreasing root biomass and root NSC. The changes in symbiotic N fixation were positively correlated with stoichiometric homeostasis of N:P ratios in intact plants without root nodules, regardless of P additions.DiscussionThis study indicates that N addition can induce relative P limitations for growth, which can stimulate rhizosphere P mobilization at the expense of root biomass and carbohydrate concentrations, reducing symbiotic N fixation in legumes. Legume species that had less changes in plant N:P ratio, such as Lespedeza daurica and Medicago varia maintained symbiotic N fixation to a greater extent under N addition. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T15:48:51Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c423f70e911b4386a57d0d0590697cf52023-04-26T15:30:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.10768941076894Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N additionQiang Li0Qiang Li1Joshua Philp2Matthew D. Denton3Yingxin Huang4Yingxin Huang5Jian Wei6Huijuan Sun7Huijuan Sun8Yang Li9Yang Li10Qian Zhao11Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Provincial Key Laboratory of Grassland Farming, Science and Technology Department of Jilin Province, Changchun, ChinaSchool of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, AustraliaNortheast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Provincial Key Laboratory of Grassland Farming, Science and Technology Department of Jilin Province, Changchun, ChinaCollege of Life Sciences, Changchun Normal University, Changchun, ChinaNortheast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, ChinaJilin Provincial Key Laboratory of Grassland Farming, Science and Technology Department of Jilin Province, Changchun, ChinaJilin Provincial Key Laboratory of Grassland Farming, Science and Technology Department of Jilin Province, Changchun, ChinaCollege of Forestry and Grassland Science, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Changchun Normal University, Changchun, ChinaIntroductionSymbiotic N fixation inhibition induced by N supply to legumes is potentially regulated by the relative N and P availability in soil. However, the specific responses of different legume species to changes in N:P availability remain unclear, and must be better understood to optimize symbiotic N fixation inputs under N enrichment. This study investigated mechanisms by which soil N and P supply influence the symbiotic N fixation of eight legume species, to quantify the inter-specific differences, and to demonstrate how these differences can be determined by the stoichiometric homeostasis in N:P ratios (HN:P).MethodsEight herbaceous legume species were grown separately in outdoor pots and treated with either no fertilizer (control), N fertilizer (14 g N m-2), P fertilizer (3.5 g P m-2) or both N and P fertilizer. Plant nutrients, stoichiometric characteristics, root biomass, non-structural carbohydrates (NSC), rhizosphere chemistry, P mobilization, root nodulation and symbiotic N fixation were measured.ResultsN addition enhanced rhizosphere P mobilization but drove a loss of root biomass and root NSC via exudation of P mobilization compound (organic acid), especially so in treatments without P addition. N addition also induced a 2-14% or 14-36% decline in symbiotic N fixation per plant biomass by legumes in treatments with or without P addition, as a result of decreasing root biomass and root NSC. The changes in symbiotic N fixation were positively correlated with stoichiometric homeostasis of N:P ratios in intact plants without root nodules, regardless of P additions.DiscussionThis study indicates that N addition can induce relative P limitations for growth, which can stimulate rhizosphere P mobilization at the expense of root biomass and carbohydrate concentrations, reducing symbiotic N fixation in legumes. Legume species that had less changes in plant N:P ratio, such as Lespedeza daurica and Medicago varia maintained symbiotic N fixation to a greater extent under N addition.https://www.frontiersin.org/articles/10.3389/fpls.2023.1076894/fullstoichiometryN:P ratiosymbiotic N fixationrhizospherephosphorus mobilizationherbaceous legume |
| spellingShingle | Qiang Li Qiang Li Joshua Philp Matthew D. Denton Yingxin Huang Yingxin Huang Jian Wei Huijuan Sun Huijuan Sun Yang Li Yang Li Qian Zhao Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition Frontiers in Plant Science stoichiometry N:P ratio symbiotic N fixation rhizosphere phosphorus mobilization herbaceous legume |
| title | Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| title_full | Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| title_fullStr | Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| title_full_unstemmed | Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| title_short | Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| title_sort | stoichiometric homeostasis of n p ratio drives species specific symbiotic n fixation inhibition under n addition |
| topic | stoichiometry N:P ratio symbiotic N fixation rhizosphere phosphorus mobilization herbaceous legume |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1076894/full |
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