In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination
This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow di...
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MDPI AG
2020-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/1/45 |
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| author | Do-Wan Lee Jae-Im Kwon Chul-Woong Woo Hwon Heo Kyung Won Kim Dong-Cheol Woo Jeong Kon Kim Dong-Hoon Lee |
| author_facet | Do-Wan Lee Jae-Im Kwon Chul-Woong Woo Hwon Heo Kyung Won Kim Dong-Cheol Woo Jeong Kon Kim Dong-Hoon Lee |
| author_sort | Do-Wan Lee |
| collection | DOAJ |
| description | This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (<i>p</i> = 0.001), gamma-aminobutyric acid (<i>p</i> = 0.019), and glutamate + glutamine (<i>p</i> = 0.001); however, creatine + phosphocreatine (<i>p</i> = 0.006) and myo-inositol (<i>p</i> = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (<i>p</i> < 0.001), glutamate/total creatine (<i>p</i> < 0.001), and GABA/total creatine (<i>p</i> = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis. |
| first_indexed | 2024-03-10T13:41:00Z |
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| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T13:41:00Z |
| publishDate | 2020-12-01 |
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| series | Diagnostics |
| spelling | doaj.art-c425ab8a10f5402bbae2752dcfb3e39e2023-11-21T03:03:13ZengMDPI AGDiagnostics2075-44182020-12-011114510.3390/diagnostics11010045In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced DemyelinationDo-Wan Lee0Jae-Im Kwon1Chul-Woong Woo2Hwon Heo3Kyung Won Kim4Dong-Cheol Woo5Jeong Kon Kim6Dong-Hoon Lee7Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, KoreaConvergence Medicine Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, KoreaConvergence Medicine Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, KoreaDepartment of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, KoreaConvergence Medicine Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, KoreaDepartment of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, KoreaDepartment of Radiation Convergence Engineering, Yonsei University, Wonju 26493, KoreaThis study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (<i>p</i> = 0.001), gamma-aminobutyric acid (<i>p</i> = 0.019), and glutamate + glutamine (<i>p</i> = 0.001); however, creatine + phosphocreatine (<i>p</i> = 0.006) and myo-inositol (<i>p</i> = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (<i>p</i> < 0.001), glutamate/total creatine (<i>p</i> < 0.001), and GABA/total creatine (<i>p</i> = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.https://www.mdpi.com/2075-4418/11/1/45rat braindemyelinationhippocampusmetabolitesmultiple sclerosis |
| spellingShingle | Do-Wan Lee Jae-Im Kwon Chul-Woong Woo Hwon Heo Kyung Won Kim Dong-Cheol Woo Jeong Kon Kim Dong-Hoon Lee In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination Diagnostics rat brain demyelination hippocampus metabolites multiple sclerosis |
| title | In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination |
| title_full | In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination |
| title_fullStr | In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination |
| title_full_unstemmed | In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination |
| title_short | In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination |
| title_sort | in vivo measurement of neurochemical abnormalities in the hippocampus in a rat model of cuprizone induced demyelination |
| topic | rat brain demyelination hippocampus metabolites multiple sclerosis |
| url | https://www.mdpi.com/2075-4418/11/1/45 |
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