Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves

To elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatmen...

Full description

Bibliographic Details
Main Authors: Aneta Krakowska-Sieprawska, Katarzyna Rafińska, Justyna Walczak-Skierska, Anna Kiełbasa, Bogusław Buszewski
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Materials
Subjects:
Online Access:https://www.mdpi.com/1996-1944/14/11/2724
_version_ 1797533165476118528
author Aneta Krakowska-Sieprawska
Katarzyna Rafińska
Justyna Walczak-Skierska
Anna Kiełbasa
Bogusław Buszewski
author_facet Aneta Krakowska-Sieprawska
Katarzyna Rafińska
Justyna Walczak-Skierska
Anna Kiełbasa
Bogusław Buszewski
author_sort Aneta Krakowska-Sieprawska
collection DOAJ
description To elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatment of plant material (pH, enzyme concentration, time, and temperature) by response surface methodology (RSM). <i>Medicago sativa</i> L. was selected as a plant material due to its richness in phenolics and flavonoids. HPLC-MS/MS analysis allowed evaluating the content of individual bioactive compounds in obtained extracts. The total content of polyphenolic compounds in the extract obtained after two-step optimization was much higher (546 ± 21 µg/g) than in the extract obtained from non-hydrolyzed material (275 ± 23 µg/g) and in the extract obtained by maceration (162 ± 20 µg/g). Furthermore, it was evidenced that extract with the highest content of polyphenolic compounds can support the cellular antioxidant system both as a free radical scavenger and by stimulating the antioxidant enzyme system.
first_indexed 2024-03-10T11:10:38Z
format Article
id doaj.art-c4262b763a72484e8394829f90c95f71
institution Directory Open Access Journal
issn 1996-1944
language English
last_indexed 2024-03-10T11:10:38Z
publishDate 2021-05-01
publisher MDPI AG
record_format Article
series Materials
spelling doaj.art-c4262b763a72484e8394829f90c95f712023-11-21T20:49:50ZengMDPI AGMaterials1996-19442021-05-011411272410.3390/ma14112724Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> LeavesAneta Krakowska-Sieprawska0Katarzyna Rafińska1Justyna Walczak-Skierska2Anna Kiełbasa3Bogusław Buszewski4Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, PolandInterdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wilenska 4 St., PL-87100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, PolandTo elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatment of plant material (pH, enzyme concentration, time, and temperature) by response surface methodology (RSM). <i>Medicago sativa</i> L. was selected as a plant material due to its richness in phenolics and flavonoids. HPLC-MS/MS analysis allowed evaluating the content of individual bioactive compounds in obtained extracts. The total content of polyphenolic compounds in the extract obtained after two-step optimization was much higher (546 ± 21 µg/g) than in the extract obtained from non-hydrolyzed material (275 ± 23 µg/g) and in the extract obtained by maceration (162 ± 20 µg/g). Furthermore, it was evidenced that extract with the highest content of polyphenolic compounds can support the cellular antioxidant system both as a free radical scavenger and by stimulating the antioxidant enzyme system.https://www.mdpi.com/1996-1944/14/11/2724enzymatic hydrolysisEA-SFEflavonoidsantioxidantHPLC-MS/MS
spellingShingle Aneta Krakowska-Sieprawska
Katarzyna Rafińska
Justyna Walczak-Skierska
Anna Kiełbasa
Bogusław Buszewski
Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
Materials
enzymatic hydrolysis
EA-SFE
flavonoids
antioxidant
HPLC-MS/MS
title Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
title_full Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
title_fullStr Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
title_full_unstemmed Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
title_short Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from <i>Medicago Sativa</i> Leaves
title_sort promising green technology in obtaining functional plant preparations combined enzyme assisted supercritical fluid extraction of flavonoids isolation from i medicago sativa i leaves
topic enzymatic hydrolysis
EA-SFE
flavonoids
antioxidant
HPLC-MS/MS
url https://www.mdpi.com/1996-1944/14/11/2724
work_keys_str_mv AT anetakrakowskasieprawska promisinggreentechnologyinobtainingfunctionalplantpreparationscombinedenzymeassistedsupercriticalfluidextractionofflavonoidsisolationfromimedicagosativaileaves
AT katarzynarafinska promisinggreentechnologyinobtainingfunctionalplantpreparationscombinedenzymeassistedsupercriticalfluidextractionofflavonoidsisolationfromimedicagosativaileaves
AT justynawalczakskierska promisinggreentechnologyinobtainingfunctionalplantpreparationscombinedenzymeassistedsupercriticalfluidextractionofflavonoidsisolationfromimedicagosativaileaves
AT annakiełbasa promisinggreentechnologyinobtainingfunctionalplantpreparationscombinedenzymeassistedsupercriticalfluidextractionofflavonoidsisolationfromimedicagosativaileaves
AT bogusławbuszewski promisinggreentechnologyinobtainingfunctionalplantpreparationscombinedenzymeassistedsupercriticalfluidextractionofflavonoidsisolationfromimedicagosativaileaves