Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa
Capacitated spermatozoa initiate fertilization by binding to the zona pellucida (ZP). Defective spermatozoa-ZP binding causes infertility. The sialyl-Lewis(x) (SLeX) sequence is the most abundant terminal sequence on the glycans of human ZP glycoproteins involving in spermatozoa-ZP binding. This stu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.700396/full |
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| author | Ying Wang Ying Wang Weie Zhao Weie Zhao Si Mei Si Mei Panyu Chen Tsz-Ying Leung Tsz-Ying Leung Cheuk-Lun Lee Cheuk-Lun Lee William S. B. Yeung William S. B. Yeung Jian-Ping Ou Jian-Ping Ou Xiaoyan Liang Philip C. N. Chiu Philip C. N. Chiu |
| author_facet | Ying Wang Ying Wang Weie Zhao Weie Zhao Si Mei Si Mei Panyu Chen Tsz-Ying Leung Tsz-Ying Leung Cheuk-Lun Lee Cheuk-Lun Lee William S. B. Yeung William S. B. Yeung Jian-Ping Ou Jian-Ping Ou Xiaoyan Liang Philip C. N. Chiu Philip C. N. Chiu |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | Capacitated spermatozoa initiate fertilization by binding to the zona pellucida (ZP). Defective spermatozoa-ZP binding causes infertility. The sialyl-Lewis(x) (SLeX) sequence is the most abundant terminal sequence on the glycans of human ZP glycoproteins involving in spermatozoa-ZP binding. This study aimed to identify and characterize the SLeX-binding proteins on human spermatozoa. By using affinity chromatography followed by mass spectrometric analysis, chromosome 1 open reading frame 56 (C1orf56) was identified to be a SLeX-binding protein of capacitated spermatozoa. The acrosomal region of spermatozoa possessed C1orf56 immunoreactive signals with intensities that increased after capacitation indicating translocation of C1orf56 to the cell surface during capacitation. Treatment with antibody against C1orf56 inhibited spermatozoa-ZP binding and ZP-induced acrosome reaction. Purified C1orf56 from capacitated spermatozoa bound to human ZP. A pilot clinical study was conducted and found no association between the percentage of capacitated spermatozoa with C1orf56 expression and in vitro fertilization (IVF) rate in assisted reproduction treatment. However, the percentage of C1orf56 positive spermatozoa in the acrosome-reacted population was significantly (P < 0.05) lower in cycles with a fertilization rate < 60% when compared to those with a higher fertilization rate, suggesting that C1orf56 may have functions after ZP-binding and acrosome reaction. A larger clinical trial is needed to determine the possible use of sperm C1orf56 content for the prediction of fertilization potential of sperm samples. |
| first_indexed | 2024-12-13T19:44:21Z |
| format | Article |
| id | doaj.art-c4263f45e37d4a18bf3ab47da368a8f6 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-13T19:44:21Z |
| publishDate | 2021-07-01 |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-c4263f45e37d4a18bf3ab47da368a8f62022-12-21T23:33:35ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-07-01910.3389/fcell.2021.700396700396Identification of Sialyl-Lewis(x)-Interacting Protein on Human SpermatozoaYing Wang0Ying Wang1Weie Zhao2Weie Zhao3Si Mei4Si Mei5Panyu Chen6Tsz-Ying Leung7Tsz-Ying Leung8Cheuk-Lun Lee9Cheuk-Lun Lee10William S. B. Yeung11William S. B. Yeung12Jian-Ping Ou13Jian-Ping Ou14Xiaoyan Liang15Philip C. N. Chiu16Philip C. N. Chiu17Department of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongDepartment of Obstetrics and Gynecology, Center of Reproductive Medicine, Peking University Third Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongThe Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongDepartment of Physiology, Medical College, Hunan University of Chinese Medicine, Changsha, ChinaThe Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongShenzhen Key Laboratory of Fertility Regulation, Department of Obstetrics and Gynecology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongShenzhen Key Laboratory of Fertility Regulation, Department of Obstetrics and Gynecology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongShenzhen Key Laboratory of Fertility Regulation, Department of Obstetrics and Gynecology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongCenter for Reproductive Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaThe Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, Hong KongShenzhen Key Laboratory of Fertility Regulation, Department of Obstetrics and Gynecology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, ChinaCapacitated spermatozoa initiate fertilization by binding to the zona pellucida (ZP). Defective spermatozoa-ZP binding causes infertility. The sialyl-Lewis(x) (SLeX) sequence is the most abundant terminal sequence on the glycans of human ZP glycoproteins involving in spermatozoa-ZP binding. This study aimed to identify and characterize the SLeX-binding proteins on human spermatozoa. By using affinity chromatography followed by mass spectrometric analysis, chromosome 1 open reading frame 56 (C1orf56) was identified to be a SLeX-binding protein of capacitated spermatozoa. The acrosomal region of spermatozoa possessed C1orf56 immunoreactive signals with intensities that increased after capacitation indicating translocation of C1orf56 to the cell surface during capacitation. Treatment with antibody against C1orf56 inhibited spermatozoa-ZP binding and ZP-induced acrosome reaction. Purified C1orf56 from capacitated spermatozoa bound to human ZP. A pilot clinical study was conducted and found no association between the percentage of capacitated spermatozoa with C1orf56 expression and in vitro fertilization (IVF) rate in assisted reproduction treatment. However, the percentage of C1orf56 positive spermatozoa in the acrosome-reacted population was significantly (P < 0.05) lower in cycles with a fertilization rate < 60% when compared to those with a higher fertilization rate, suggesting that C1orf56 may have functions after ZP-binding and acrosome reaction. A larger clinical trial is needed to determine the possible use of sperm C1orf56 content for the prediction of fertilization potential of sperm samples.https://www.frontiersin.org/articles/10.3389/fcell.2021.700396/fullzona pellucidahuman spermatozoasialyl-Lewis(x)C1orf56fertilization rate |
| spellingShingle | Ying Wang Ying Wang Weie Zhao Weie Zhao Si Mei Si Mei Panyu Chen Tsz-Ying Leung Tsz-Ying Leung Cheuk-Lun Lee Cheuk-Lun Lee William S. B. Yeung William S. B. Yeung Jian-Ping Ou Jian-Ping Ou Xiaoyan Liang Philip C. N. Chiu Philip C. N. Chiu Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa Frontiers in Cell and Developmental Biology zona pellucida human spermatozoa sialyl-Lewis(x) C1orf56 fertilization rate |
| title | Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa |
| title_full | Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa |
| title_fullStr | Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa |
| title_full_unstemmed | Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa |
| title_short | Identification of Sialyl-Lewis(x)-Interacting Protein on Human Spermatozoa |
| title_sort | identification of sialyl lewis x interacting protein on human spermatozoa |
| topic | zona pellucida human spermatozoa sialyl-Lewis(x) C1orf56 fertilization rate |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.700396/full |
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