Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection
A sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Pos...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-05-01
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| Series: | Separations |
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| Online Access: | http://www.mdpi.com/2297-8739/4/2/16 |
| _version_ | 1798026797429817344 |
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| author | Peter H. Tang |
| author_facet | Peter H. Tang |
| author_sort | Peter H. Tang |
| collection | DOAJ |
| description | A sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Posaconazole and internal standard ketoconazole in the methanol extract are subsequently analyzed by using a fluorescence (FL) detector at optimized wavelengths (excitation 245 nm and emission 380 nm). The method achieves a linear detector response for peak height measurements over the concentration range of 0.1–10 µg/mL which adequately covers the therapeutic range for appropriate patient monitoring. The chromatographic time is less than 8 min per injection, an improvement over most published HPLC/FL or HPLC/UV methods. The method’s limit of quantitation, linearity, imprecision, and accuracy met all criteria required by the Guidance for Industry Bioanalytical Method Validation. In comparison to other published methods, the current method would be of interest to analytical and clinical laboratories because it employs simple, rapid, and cost-effective procedures. |
| first_indexed | 2024-04-11T18:41:09Z |
| format | Article |
| id | doaj.art-c426bfca7606403bace6bbeac512a80e |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-11T18:41:09Z |
| publishDate | 2017-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-c426bfca7606403bace6bbeac512a80e2022-12-22T04:08:58ZengMDPI AGSeparations2297-87392017-05-01421610.3390/separations4020016separations4020016Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence DetectionPeter H. Tang0Departments of Pediatrics, Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USAA sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Posaconazole and internal standard ketoconazole in the methanol extract are subsequently analyzed by using a fluorescence (FL) detector at optimized wavelengths (excitation 245 nm and emission 380 nm). The method achieves a linear detector response for peak height measurements over the concentration range of 0.1–10 µg/mL which adequately covers the therapeutic range for appropriate patient monitoring. The chromatographic time is less than 8 min per injection, an improvement over most published HPLC/FL or HPLC/UV methods. The method’s limit of quantitation, linearity, imprecision, and accuracy met all criteria required by the Guidance for Industry Bioanalytical Method Validation. In comparison to other published methods, the current method would be of interest to analytical and clinical laboratories because it employs simple, rapid, and cost-effective procedures.http://www.mdpi.com/2297-8739/4/2/16posaconazoleketoconazoleHPLCfluorescence |
| spellingShingle | Peter H. Tang Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection Separations posaconazole ketoconazole HPLC fluorescence |
| title | Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
| title_full | Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
| title_fullStr | Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
| title_full_unstemmed | Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
| title_short | Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection |
| title_sort | determination of posaconazole in plasma serum by high performance liquid chromatography with fluorescence detection |
| topic | posaconazole ketoconazole HPLC fluorescence |
| url | http://www.mdpi.com/2297-8739/4/2/16 |
| work_keys_str_mv | AT peterhtang determinationofposaconazoleinplasmaserumbyhighperformanceliquidchromatographywithfluorescencedetection |