BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event

BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event

Determining which microorganisms are active within soil communities remains a major technical endeavor in microbial ecology research. One promising method to accomplish this is coupling bioorthogonal non-canonical amino acid tagging (BONCAT) with fluorescence activated cell sorting (FACS) which sort...

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Bibliographic Details
Main Authors: Ryan V. Trexler, Marc W. Van Goethem, Danielle Goudeau, Nandita Nath, Rex R. Malmstrom, Trent R. Northen, Estelle Couradeau
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Microbiology
Subjects:
BONCAT
biocrust
soil metagenomics
active microorganisms
soil wetting
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1176751/full

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