Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability
Histone methylation levels, which are determined by the action of both histone demethylases and methyltransferases, impact multiple biological processes by affecting gene expression activity. Methionine metabolism generates the major methyl donor S-adenosylmethionine (SAM) for histone methylation. T...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2016-01-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.115.024273 |
| _version_ | 1818724163603398656 |
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| author | Mengying Liu Valerie L. Barnes Lori A. Pile |
| author_facet | Mengying Liu Valerie L. Barnes Lori A. Pile |
| author_sort | Mengying Liu |
| collection | DOAJ |
| description | Histone methylation levels, which are determined by the action of both histone demethylases and methyltransferases, impact multiple biological processes by affecting gene expression activity. Methionine metabolism generates the major methyl donor S-adenosylmethionine (SAM) for histone methylation. The functions of methionine metabolic enzymes in regulating biological processes as well as the interaction between the methionine pathway and histone methylation, however, are still not fully understood. Here, we report that reduced levels of some enzymes involved in methionine metabolism and histone demethylases lead to lethality as well as wing development and cell proliferation defects in Drosophila melanogaster. Additionally, disruption of methionine metabolism can directly affect histone methylation levels. Reduction of little imaginal discs (LID) histone demethylase, but not lysine-specific demethylase 2 (KDM2) demethylase, is able to counter the effects on histone methylation due to reduction of SAM synthetase (SAM-S). Taken together, these results reveal an essential role of key enzymes that control methionine metabolism and histone methylation. Additionally, these findings are an indication of a strong connection between metabolism and epigenetics. |
| first_indexed | 2024-12-17T21:22:03Z |
| format | Article |
| id | doaj.art-c4287c357f884000870dadf7431ffa78 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-17T21:22:03Z |
| publishDate | 2016-01-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-c4287c357f884000870dadf7431ffa782022-12-21T21:32:11ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362016-01-016112113210.1534/g3.115.02427312Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of ViabilityMengying LiuValerie L. BarnesLori A. PileHistone methylation levels, which are determined by the action of both histone demethylases and methyltransferases, impact multiple biological processes by affecting gene expression activity. Methionine metabolism generates the major methyl donor S-adenosylmethionine (SAM) for histone methylation. The functions of methionine metabolic enzymes in regulating biological processes as well as the interaction between the methionine pathway and histone methylation, however, are still not fully understood. Here, we report that reduced levels of some enzymes involved in methionine metabolism and histone demethylases lead to lethality as well as wing development and cell proliferation defects in Drosophila melanogaster. Additionally, disruption of methionine metabolism can directly affect histone methylation levels. Reduction of little imaginal discs (LID) histone demethylase, but not lysine-specific demethylase 2 (KDM2) demethylase, is able to counter the effects on histone methylation due to reduction of SAM synthetase (SAM-S). Taken together, these results reveal an essential role of key enzymes that control methionine metabolism and histone methylation. Additionally, these findings are an indication of a strong connection between metabolism and epigenetics.http://g3journal.org/lookup/doi/10.1534/g3.115.024273methionine metabolismhistone methyltransferasehistone demethylase Drosophila |
| spellingShingle | Mengying Liu Valerie L. Barnes Lori A. Pile Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability G3: Genes, Genomes, Genetics methionine metabolism histone methyltransferase histone demethylase Drosophila |
| title | Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability |
| title_full | Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability |
| title_fullStr | Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability |
| title_full_unstemmed | Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability |
| title_short | Disruption of Methionine Metabolism in Drosophila melanogaster Impacts Histone Methylation and Results in Loss of Viability |
| title_sort | disruption of methionine metabolism in drosophila melanogaster impacts histone methylation and results in loss of viability |
| topic | methionine metabolism histone methyltransferase histone demethylase Drosophila |
| url | http://g3journal.org/lookup/doi/10.1534/g3.115.024273 |
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