Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase chain reaction assay by using a species-speci...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2006-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/12/3/05-0888_article |
| _version_ | 1818496088937594880 |
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| author | Sanela Svraka Jean-Marc Rolain Yassina Bechah John Gatabazi Didier Raoult |
| author_facet | Sanela Svraka Jean-Marc Rolain Yassina Bechah John Gatabazi Didier Raoult |
| author_sort | Sanela Svraka |
| collection | DOAJ |
| description | Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase chain reaction assay by using a species-specific probe targeting the gltA gene. This assay, which was rapid, specific for R. prowazekii only, and sensitive (cutoff detection of 1 to 5 copies per sample), detected and directly identified R. prowazekii in blood of 12 experimentally infected mice sampled at day 3 and 6 postinfection or in naturally or experimentally infected lice. Because our assay is highly standardized and easily adaptable, it could improve epidemic typhus surveillance in public health programs, especially for countries with underdiagnosed or unrecognized human cases. |
| first_indexed | 2024-12-10T18:28:50Z |
| format | Article |
| id | doaj.art-c428b2c8eab24f8d95fde84c7221c82a |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-10T18:28:50Z |
| publishDate | 2006-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-c428b2c8eab24f8d95fde84c7221c82a2022-12-22T01:38:00ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592006-03-0112342843210.3201/eid1203.050888Rickettsia prowazekii and Real-time Polymerase Chain ReactionSanela SvrakaJean-Marc RolainYassina BechahJohn GatabaziDidier RaoultRickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase chain reaction assay by using a species-specific probe targeting the gltA gene. This assay, which was rapid, specific for R. prowazekii only, and sensitive (cutoff detection of 1 to 5 copies per sample), detected and directly identified R. prowazekii in blood of 12 experimentally infected mice sampled at day 3 and 6 postinfection or in naturally or experimentally infected lice. Because our assay is highly standardized and easily adaptable, it could improve epidemic typhus surveillance in public health programs, especially for countries with underdiagnosed or unrecognized human cases.https://wwwnc.cdc.gov/eid/article/12/3/05-0888_articleRickettsia prowazekiireal-time PCRepidemic typhusbioterrorismresearchRwanda |
| spellingShingle | Sanela Svraka Jean-Marc Rolain Yassina Bechah John Gatabazi Didier Raoult Rickettsia prowazekii and Real-time Polymerase Chain Reaction Emerging Infectious Diseases Rickettsia prowazekii real-time PCR epidemic typhus bioterrorism research Rwanda |
| title | Rickettsia prowazekii and Real-time Polymerase Chain Reaction |
| title_full | Rickettsia prowazekii and Real-time Polymerase Chain Reaction |
| title_fullStr | Rickettsia prowazekii and Real-time Polymerase Chain Reaction |
| title_full_unstemmed | Rickettsia prowazekii and Real-time Polymerase Chain Reaction |
| title_short | Rickettsia prowazekii and Real-time Polymerase Chain Reaction |
| title_sort | rickettsia prowazekii and real time polymerase chain reaction |
| topic | Rickettsia prowazekii real-time PCR epidemic typhus bioterrorism research Rwanda |
| url | https://wwwnc.cdc.gov/eid/article/12/3/05-0888_article |
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