Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew
Downy mildew caused by the obligate parasite <i>Hyaloperonospora brassicae</i> is a devastating disease for <i>Brassica</i> species. Infection of <i>Hyaloperonospora brassicae</i> often leads to yellow spots on leaves, which significantly impacts quality and yield...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/21/15710 |
| Summary: | Downy mildew caused by the obligate parasite <i>Hyaloperonospora brassicae</i> is a devastating disease for <i>Brassica</i> species. Infection of <i>Hyaloperonospora brassicae</i> often leads to yellow spots on leaves, which significantly impacts quality and yield of pakchoi. In the present study, we conducted a comparative transcriptome between the resistant and susceptible pakchoi cultivars in response to <i>Hyaloperonospora brassicae</i> infection. A total of 1073 disease-resistance-related differentially expressed genes were identified using a Venn diagram. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these genes were mainly involved in plant−pathogen interaction, plant hormone signal transduction, and other photosynthesis-related metabolic processes. Analysis of the phytohormone content revealed that salicylic acid increased significantly in the resistant material after inoculation with <i>Hyaloperonospora brassicae</i>, whereas the contents of jasmonic acid, abscisic acid, and 1-aminocyclopropane-1-carboxylic acid decreased. Exogenous salicylic acid treatment also significantly upregulated <i>Hyaloperonospora brassicae</i>-induced genes, which further confirmed a crucial role of salicylic acid during pakchoi defense against <i>Hyaloperonospora brassicae</i>. Based on these findings, we suggest that the salicylic-acid-mediated signal transduction contributes to the resistance of pakchoi to downy mildew, and <i>PAL1, ICS1, NPR1, PR1, PR5, WRKY70, WRKY33, CML43, CNGC9,</i> and <i>CDPK15</i> were involved in this responsive process. Our findings evidently contribute to revealing the molecular mechanism of pakchoi defense against <i>Hyaloperonospora brassicae</i>. |
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| ISSN: | 1661-6596 1422-0067 |