Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew
Downy mildew caused by the obligate parasite <i>Hyaloperonospora brassicae</i> is a devastating disease for <i>Brassica</i> species. Infection of <i>Hyaloperonospora brassicae</i> often leads to yellow spots on leaves, which significantly impacts quality and yield...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15710 |
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| author | Yaosong Chen Liming Miao Xiaofeng Li Yiwen Liu Dandan Xi Dingyu Zhang Lu Gao Yuying Zhu Shaojun Dai Hongfang Zhu |
| author_facet | Yaosong Chen Liming Miao Xiaofeng Li Yiwen Liu Dandan Xi Dingyu Zhang Lu Gao Yuying Zhu Shaojun Dai Hongfang Zhu |
| author_sort | Yaosong Chen |
| collection | DOAJ |
| description | Downy mildew caused by the obligate parasite <i>Hyaloperonospora brassicae</i> is a devastating disease for <i>Brassica</i> species. Infection of <i>Hyaloperonospora brassicae</i> often leads to yellow spots on leaves, which significantly impacts quality and yield of pakchoi. In the present study, we conducted a comparative transcriptome between the resistant and susceptible pakchoi cultivars in response to <i>Hyaloperonospora brassicae</i> infection. A total of 1073 disease-resistance-related differentially expressed genes were identified using a Venn diagram. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these genes were mainly involved in plant−pathogen interaction, plant hormone signal transduction, and other photosynthesis-related metabolic processes. Analysis of the phytohormone content revealed that salicylic acid increased significantly in the resistant material after inoculation with <i>Hyaloperonospora brassicae</i>, whereas the contents of jasmonic acid, abscisic acid, and 1-aminocyclopropane-1-carboxylic acid decreased. Exogenous salicylic acid treatment also significantly upregulated <i>Hyaloperonospora brassicae</i>-induced genes, which further confirmed a crucial role of salicylic acid during pakchoi defense against <i>Hyaloperonospora brassicae</i>. Based on these findings, we suggest that the salicylic-acid-mediated signal transduction contributes to the resistance of pakchoi to downy mildew, and <i>PAL1, ICS1, NPR1, PR1, PR5, WRKY70, WRKY33, CML43, CNGC9,</i> and <i>CDPK15</i> were involved in this responsive process. Our findings evidently contribute to revealing the molecular mechanism of pakchoi defense against <i>Hyaloperonospora brassicae</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:29:06Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-c428cb43dc074bb89fc8b3c02de716b22023-11-10T15:04:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211571010.3390/ijms242115710Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy MildewYaosong Chen0Liming Miao1Xiaofeng Li2Yiwen Liu3Dandan Xi4Dingyu Zhang5Lu Gao6Yuying Zhu7Shaojun Dai8Hongfang Zhu9Shanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Agricultural Science and Technology Information, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 201418, ChinaShanghai Key Laboratory of Protected Horticultural Technology, Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaDowny mildew caused by the obligate parasite <i>Hyaloperonospora brassicae</i> is a devastating disease for <i>Brassica</i> species. Infection of <i>Hyaloperonospora brassicae</i> often leads to yellow spots on leaves, which significantly impacts quality and yield of pakchoi. In the present study, we conducted a comparative transcriptome between the resistant and susceptible pakchoi cultivars in response to <i>Hyaloperonospora brassicae</i> infection. A total of 1073 disease-resistance-related differentially expressed genes were identified using a Venn diagram. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these genes were mainly involved in plant−pathogen interaction, plant hormone signal transduction, and other photosynthesis-related metabolic processes. Analysis of the phytohormone content revealed that salicylic acid increased significantly in the resistant material after inoculation with <i>Hyaloperonospora brassicae</i>, whereas the contents of jasmonic acid, abscisic acid, and 1-aminocyclopropane-1-carboxylic acid decreased. Exogenous salicylic acid treatment also significantly upregulated <i>Hyaloperonospora brassicae</i>-induced genes, which further confirmed a crucial role of salicylic acid during pakchoi defense against <i>Hyaloperonospora brassicae</i>. Based on these findings, we suggest that the salicylic-acid-mediated signal transduction contributes to the resistance of pakchoi to downy mildew, and <i>PAL1, ICS1, NPR1, PR1, PR5, WRKY70, WRKY33, CML43, CNGC9,</i> and <i>CDPK15</i> were involved in this responsive process. Our findings evidently contribute to revealing the molecular mechanism of pakchoi defense against <i>Hyaloperonospora brassicae</i>.https://www.mdpi.com/1422-0067/24/21/15710pakchoidowny mildewtranscriptomedifferentially expressed genessalicylic acid |
| spellingShingle | Yaosong Chen Liming Miao Xiaofeng Li Yiwen Liu Dandan Xi Dingyu Zhang Lu Gao Yuying Zhu Shaojun Dai Hongfang Zhu Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew International Journal of Molecular Sciences pakchoi downy mildew transcriptome differentially expressed genes salicylic acid |
| title | Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew |
| title_full | Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew |
| title_fullStr | Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew |
| title_full_unstemmed | Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew |
| title_short | Comparative Transcriptome Analysis between Resistant and Susceptible Pakchoi Cultivars in Response to Downy Mildew |
| title_sort | comparative transcriptome analysis between resistant and susceptible pakchoi cultivars in response to downy mildew |
| topic | pakchoi downy mildew transcriptome differentially expressed genes salicylic acid |
| url | https://www.mdpi.com/1422-0067/24/21/15710 |
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