IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters
Abstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10...
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Sociedade Brasileira de Pesquisa Odontológica
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| Series: | Brazilian Oral Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=en |
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| author | Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ |
| author_facet | Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ |
| author_sort | Emine PIRIM GORGUN |
| collection | DOAJ |
| description | Abstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay(ELISA).GAgP patients showed significant improvement on clinical parameters after periodontal therapy(p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levelswere observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6(-174) and IL-10(-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6(-174) GG genotype had higher levels of GCF IL-10 levels. |
| first_indexed | 2024-12-11T01:41:45Z |
| format | Article |
| id | doaj.art-c428df3f6b564f56b3b4ca011c44eab8 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-11T01:41:45Z |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-c428df3f6b564f56b3b4ca011c44eab82022-12-22T01:25:02ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-310731010.1590/1807-3107bor-2017.vol31.0012S1806-83242017000100209IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parametersEmine PIRIM GORGUNHulya TOKERErtan Mahir KORKMAZOmer POYRAZAbstract Genetic variations observed in cytokines affect periodontitis susceptibility. The aim of this study was to investigate interleukin(IL)-6(-174) and IL-10(-597) gene polymorphisms in generalized aggressive periodontitis (GAgP) patients. Also, we aimed to evaluate the effects of IL-6 and IL-10 gene polymorphisms on the clinical outcomes of non-surgical periodontal therapy and cytokine levels in gingival crevicular fluid(GCF) and serum. Fifty-three patients with GAgP and 50 periodontally healthy individuals were included in this study. Clinical parameters, GCF and blood samples were collected at baseline and at 6-week. Non-surgical periodontal therapy was performed in patients with GAgP. Gene analysis were determined by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and cytokine levels were determined by enzyme-linked immunosorbent assay(ELISA).GAgP patients showed significant improvement on clinical parameters after periodontal therapy(p<0.05). In the GAgP group, IL-6 GG genotype and G allele frequency were higher than in the control group. GCF IL-6 level was also significantly lower at 6-week in the GAgP group. Higher GCF IL-10 levelswere observed in patients carrying the IL-6 GG genotype than in those carrying the GC+CC genotype at baseline. In conclusion, IL-6(-174) and IL-10(-597) gene polymorphisms were found to be associated with GAgP and genotype distribution did not affect the outcome of non-surgical periodontal therapy, while patients with IL-6(-174) GG genotype had higher levels of GCF IL-10 levels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=enInterleukin-6Interleukin-10Polymorphism, GeneticAggressive Periodontitis |
| spellingShingle | Emine PIRIM GORGUN Hulya TOKER Ertan Mahir KORKMAZ Omer POYRAZ IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters Brazilian Oral Research Interleukin-6 Interleukin-10 Polymorphism, Genetic Aggressive Periodontitis |
| title | IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
| title_full | IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
| title_fullStr | IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
| title_full_unstemmed | IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
| title_short | IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters |
| title_sort | il 6 and il 10 gene polymorphisms in patients with aggressive periodontitis effects on gcf serum and clinic parameters |
| topic | Interleukin-6 Interleukin-10 Polymorphism, Genetic Aggressive Periodontitis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242017000100209&lng=en&tlng=en |
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