In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells
Propolis has been used in folk medicine for centuries due to its healing properties. Ethanolic extracts of propolis (EEP) are rich sources of phenolic acid and flavonoids. Natural phenolic compounds may exert chemoprotective activity in cancer cells due to their ability to scavenge free rad...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500019M.pdf |
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| author | Milošević-Đorđević Olivera Grujičić Darko Radović Marina Vuković Nenad Žižić Jovana Marković Snežana |
| author_facet | Milošević-Đorđević Olivera Grujičić Darko Radović Marina Vuković Nenad Žižić Jovana Marković Snežana |
| author_sort | Milošević-Đorđević Olivera |
| collection | DOAJ |
| description | Propolis has been used in folk medicine for centuries due to its healing
properties. Ethanolic extracts of propolis (EEP) are rich sources of phenolic
acid and flavonoids. Natural phenolic compounds may exert chemoprotective
activity in cancer cells due to their ability to scavenge free radicals. The
aim of this in vitro study was to investigate the genotoxic and
anti-mutagenic effects of the EEP on human peripheral blood lymphocytes
(PBLs) and their cytotoxic potential on the human breast cancer cell line
(MDA-MB-231 cells). Both cell cultures were treated with six concentrations
(1, 10, 50, 100, 250 and 500 μg/ml) of EEP1 and EEP2, separately and in
combination with mitomycin C (MMC). Our results show that the EEP1 and EEP2
samples of propolis after separate and combined treatments with MMC did not
influence the nuclear division index (NDI). In the combined treatment, both
tested EEPs significantly reduced MMC-induced micronuclei (MN) in PBLs. At 48
h after exposure of the MDA-MB-231 cell line to a combined treatment of EEP
samples with MMC, the IC50 values were significantly reduced (23.79 and 19.13
μg/ml, for EEP1+MMC and EEP2+MMC, respectively, in comparison to the single
treatment. In conclusion, the tested ethanolic extracts of propolis exhibited
a certain level of in vitro antimutagenic activity in PBLs from healthy
subjects, and anticancer activity in breast cancer cell line. The presented
findings suggest that the ethanolic extracts of propolis show potential in
anticancer therapeutic strategy. [Projekat Ministarstva nauke Republike
Srbije, br. III41010] |
| first_indexed | 2024-04-13T11:36:34Z |
| format | Article |
| id | doaj.art-c42b31233183433db2310a7aaa9d5240 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-13T11:36:34Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-c42b31233183433db2310a7aaa9d52402022-12-22T02:48:26ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167257158110.2298/ABS141013019M0354-46641500019MIn vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cellsMilošević-Đorđević Olivera0Grujičić Darko1Radović Marina2Vuković Nenad3Žižić Jovana4Marković Snežana5Faculty of Science, Kragujevac + Faculty of Medical Sciences, KragujevacFaculty of Science, KragujevacFaculty of Science, KragujevacFaculty of Science, KragujevacFaculty of Science, KragujevacFaculty of Science, KragujevacPropolis has been used in folk medicine for centuries due to its healing properties. Ethanolic extracts of propolis (EEP) are rich sources of phenolic acid and flavonoids. Natural phenolic compounds may exert chemoprotective activity in cancer cells due to their ability to scavenge free radicals. The aim of this in vitro study was to investigate the genotoxic and anti-mutagenic effects of the EEP on human peripheral blood lymphocytes (PBLs) and their cytotoxic potential on the human breast cancer cell line (MDA-MB-231 cells). Both cell cultures were treated with six concentrations (1, 10, 50, 100, 250 and 500 μg/ml) of EEP1 and EEP2, separately and in combination with mitomycin C (MMC). Our results show that the EEP1 and EEP2 samples of propolis after separate and combined treatments with MMC did not influence the nuclear division index (NDI). In the combined treatment, both tested EEPs significantly reduced MMC-induced micronuclei (MN) in PBLs. At 48 h after exposure of the MDA-MB-231 cell line to a combined treatment of EEP samples with MMC, the IC50 values were significantly reduced (23.79 and 19.13 μg/ml, for EEP1+MMC and EEP2+MMC, respectively, in comparison to the single treatment. In conclusion, the tested ethanolic extracts of propolis exhibited a certain level of in vitro antimutagenic activity in PBLs from healthy subjects, and anticancer activity in breast cancer cell line. The presented findings suggest that the ethanolic extracts of propolis show potential in anticancer therapeutic strategy. [Projekat Ministarstva nauke Republike Srbije, br. III41010]http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500019M.pdfpropolishuman lymphocytesgenotoxicitycytotoxicityMDA-MB-231 cell line |
| spellingShingle | Milošević-Đorđević Olivera Grujičić Darko Radović Marina Vuković Nenad Žižić Jovana Marković Snežana In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells Archives of Biological Sciences propolis human lymphocytes genotoxicity cytotoxicity MDA-MB-231 cell line |
| title | In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| title_full | In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| title_fullStr | In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| title_full_unstemmed | In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| title_short | In vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| title_sort | in vitro chemoprotective and anticancer activities of propolis in human lymphocytes and breast cancer cells |
| topic | propolis human lymphocytes genotoxicity cytotoxicity MDA-MB-231 cell line |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500019M.pdf |
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