Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
Extra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.770130/full |
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| author | Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito |
| author_facet | Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito |
| author_sort | Samiratu Mahazu |
| collection | DOAJ |
| description | Extra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli isolates from two Ghanaian hospitals. We also investigated the isolates within a global context to provide further insight into the dissemination of this highly pathogenic clone. A phylogenetic tree of the 21 isolate genomes, along with 102 others from global collection, was constructed representing the ensuing clades and sub-clades of the ST: A/H53, B2/H24R, B3/H24Rx, and B4/H24RxC. The carbapenem-resistant sub-clade B4/H24RxC is reported to have emerged in the early 2000s when ST410 acquired an IncX3 plasmid carrying a blaOXA–181 carbapenemase gene, and a second carbapenemase gene, blaNDM–5, on a conserved IncFII plasmid in 2014. We identified, in this study, one blaOXA–181–carrying isolate belonging to B4/H24RxC sub-lineage and one carrying blaNDM–1 belonging to sub-lineage B3/H24Rx. The blaOXA–181 gene was found on a 51kb IncX3 plasmid; pEc1079_3. The majority (12/21) of our Ghanaian isolates were clustered with international strains described by previous authors as closely related strains to B4/H24RxC. Six others were clustered among the ESBL-associated sub-lineage B3/H24Rx and three with the globally disseminated sub-lineage B4/H24RxC. The results show that this highly pathogenic clone is disseminated in Ghana and, given its ability to transmit between hosts, it poses a serious threat and should be monitored closely. |
| first_indexed | 2024-12-23T04:05:42Z |
| format | Article |
| id | doaj.art-c42ca45a6467457a960cc236579368b2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T04:05:42Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c42ca45a6467457a960cc236579368b22022-12-21T18:00:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-12-011210.3389/fmicb.2021.770130770130Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in GhanaSamiratu Mahazu0Samiratu Mahazu1Isaac Prah2Isaac Prah3Alafate Ayibieke4Wakana Sato5Takaya Hayashi6Toshihiko Suzuki7Shiroh Iwanaga8Anthony Ablordey9Ryoichi Saito10Department of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Environmental Parasitology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Virology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Molecular Virology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Bacterial Pathogenesis, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Environmental Parasitology, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, JapanExtra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli isolates from two Ghanaian hospitals. We also investigated the isolates within a global context to provide further insight into the dissemination of this highly pathogenic clone. A phylogenetic tree of the 21 isolate genomes, along with 102 others from global collection, was constructed representing the ensuing clades and sub-clades of the ST: A/H53, B2/H24R, B3/H24Rx, and B4/H24RxC. The carbapenem-resistant sub-clade B4/H24RxC is reported to have emerged in the early 2000s when ST410 acquired an IncX3 plasmid carrying a blaOXA–181 carbapenemase gene, and a second carbapenemase gene, blaNDM–5, on a conserved IncFII plasmid in 2014. We identified, in this study, one blaOXA–181–carrying isolate belonging to B4/H24RxC sub-lineage and one carrying blaNDM–1 belonging to sub-lineage B3/H24Rx. The blaOXA–181 gene was found on a 51kb IncX3 plasmid; pEc1079_3. The majority (12/21) of our Ghanaian isolates were clustered with international strains described by previous authors as closely related strains to B4/H24RxC. Six others were clustered among the ESBL-associated sub-lineage B3/H24Rx and three with the globally disseminated sub-lineage B4/H24RxC. The results show that this highly pathogenic clone is disseminated in Ghana and, given its ability to transmit between hosts, it poses a serious threat and should be monitored closely.https://www.frontiersin.org/articles/10.3389/fmicb.2021.770130/fullExtra-intestinal pathogenic E. coli (ExPEC)ST410B3/H24RxB4H24RxCOxacillinase-181 (OXA-181) |
| spellingShingle | Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana Frontiers in Microbiology Extra-intestinal pathogenic E. coli (ExPEC) ST410 B3/H24Rx B4H24RxC Oxacillinase-181 (OXA-181) |
| title | Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana |
| title_full | Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana |
| title_fullStr | Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana |
| title_full_unstemmed | Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana |
| title_short | Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana |
| title_sort | possible dissemination of escherichia coli sequence type 410 closely related to b4 h24rxc in ghana |
| topic | Extra-intestinal pathogenic E. coli (ExPEC) ST410 B3/H24Rx B4H24RxC Oxacillinase-181 (OXA-181) |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.770130/full |
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