Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
This study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gib...
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| Format: | Article |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1319698/full |
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| author | Yingying Du Xiaowen Lin Xianping Shao Xianping Shao Xianping Shao Jianhua Zhao Jianhua Zhao Jianhua Zhao Hong Xu Hong Xu Hong Xu Clement R. de Cruz Qiyou Xu Qiyou Xu Qiyou Xu |
| author_facet | Yingying Du Xiaowen Lin Xianping Shao Xianping Shao Xianping Shao Jianhua Zhao Jianhua Zhao Jianhua Zhao Hong Xu Hong Xu Hong Xu Clement R. de Cruz Qiyou Xu Qiyou Xu Qiyou Xu |
| author_sort | Yingying Du |
| collection | DOAJ |
| description | This study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gibeilo). A high-plant protein diet was formulated and used as a basal diet and supplemented with five different levels of coated Met at 0.15, 0.30, 0.45, 0.60 and 0.75%, corresponding to final analyzed Met levels of 0.34, 0.49, 0.64, 0.76, 0.92 and 1.06%. Three replicate groups of fish (initial mean weight, 11.37 ± 0.02 g) (20 fish per replicate) were fed the test diets over a 10-week feeding period. The results indicated that with the increase of coated Met level, the final weight, weight gain (WG) and specific growth rate initially boosted and then suppressed, peaking at 0.76% Met level (P< 0.05). Increasing dietary Met level led to significantly increased muscle crude protein content (P< 0.05) and reduced serum alanine aminotransferase activity (P< 0.05). Using appropriate dietary Met level led to reduced malondialdehyde concentration in hepatopancreas (P< 0.05), improved superoxide dismutase activity (P< 0.05), and enhanced intestinal amylase and protease activities (P< 0.05). The expression levels of genes associated with muscle protein synthesis such as insulin-like growth factor-1, protein kinase B, target of rapamycin and eukaryotic initiation factor 4E binding protein-1 mRNA were significantly regulated, peaking at Met level of 0.76% (P< 0.05). In conclusion, supplementing optimal level of coated Met improved on fish growth, antioxidant capacity, and the expression of TOR pathway related genes in muscle. The optimal dietary Met level was determined to be 0.71% of the diet based on quadratic regression analysis of WG. |
| first_indexed | 2024-04-24T13:12:06Z |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-24T13:12:06Z |
| publishDate | 2024-04-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-c4312eaf28504d22b84556c48fb380d12024-04-05T04:20:54ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-04-011510.3389/fimmu.2024.13196981319698Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelioYingying Du0Xiaowen Lin1Xianping Shao2Xianping Shao3Xianping Shao4Jianhua Zhao5Jianhua Zhao6Jianhua Zhao7Hong Xu8Hong Xu9Hong Xu10Clement R. de Cruz11Qiyou Xu12Qiyou Xu13Qiyou Xu14College of Life Science, Huzhou University, Huzhou, ChinaCollege of Life Science, Huzhou University, Huzhou, ChinaCollege of Life Science, Huzhou University, Huzhou, ChinaZhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, Huzhou University, Huzhou, ChinaNation Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou Universityy, Huzhou, ChinaCollege of Life Science, Huzhou University, Huzhou, ChinaZhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, Huzhou University, Huzhou, ChinaNation Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou Universityy, Huzhou, ChinaCollege of Life Science, Huzhou University, Huzhou, ChinaZhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, Huzhou University, Huzhou, ChinaNation Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou Universityy, Huzhou, ChinaLaboratory of Sustainable Aquaculture, International Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia, Port Dickson, Negeri Sembilan, MalaysiaCollege of Life Science, Huzhou University, Huzhou, ChinaZhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, Huzhou University, Huzhou, ChinaNation Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Huzhou Universityy, Huzhou, ChinaThis study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gibeilo). A high-plant protein diet was formulated and used as a basal diet and supplemented with five different levels of coated Met at 0.15, 0.30, 0.45, 0.60 and 0.75%, corresponding to final analyzed Met levels of 0.34, 0.49, 0.64, 0.76, 0.92 and 1.06%. Three replicate groups of fish (initial mean weight, 11.37 ± 0.02 g) (20 fish per replicate) were fed the test diets over a 10-week feeding period. The results indicated that with the increase of coated Met level, the final weight, weight gain (WG) and specific growth rate initially boosted and then suppressed, peaking at 0.76% Met level (P< 0.05). Increasing dietary Met level led to significantly increased muscle crude protein content (P< 0.05) and reduced serum alanine aminotransferase activity (P< 0.05). Using appropriate dietary Met level led to reduced malondialdehyde concentration in hepatopancreas (P< 0.05), improved superoxide dismutase activity (P< 0.05), and enhanced intestinal amylase and protease activities (P< 0.05). The expression levels of genes associated with muscle protein synthesis such as insulin-like growth factor-1, protein kinase B, target of rapamycin and eukaryotic initiation factor 4E binding protein-1 mRNA were significantly regulated, peaking at Met level of 0.76% (P< 0.05). In conclusion, supplementing optimal level of coated Met improved on fish growth, antioxidant capacity, and the expression of TOR pathway related genes in muscle. The optimal dietary Met level was determined to be 0.71% of the diet based on quadratic regression analysis of WG.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1319698/fullcoated methioninegrowth performancedigestive enzyme activityTOR signaling pathwaygibel carp |
| spellingShingle | Yingying Du Xiaowen Lin Xianping Shao Xianping Shao Xianping Shao Jianhua Zhao Jianhua Zhao Jianhua Zhao Hong Xu Hong Xu Hong Xu Clement R. de Cruz Qiyou Xu Qiyou Xu Qiyou Xu Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio Frontiers in Immunology coated methionine growth performance digestive enzyme activity TOR signaling pathway gibel carp |
| title | Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio |
| title_full | Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio |
| title_fullStr | Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio |
| title_full_unstemmed | Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio |
| title_short | Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio |
| title_sort | effects of supplementing coated methionine in a high plant protein diet on growth antioxidant capacity digestive enzymes activity and expression of tor signaling pathway associated genes in gibel carp carassius auratus gibelio |
| topic | coated methionine growth performance digestive enzyme activity TOR signaling pathway gibel carp |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1319698/full |
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