Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma
Multiple myeloma (MM) is one of the plasma cell-related hematological malignancies exceeding 10.0% of all marrow cells, and they make a paraprotein that is a marker of the disease. Myeloma is one of the most common types of hematological malignancies in humans. Genetic bio-markers have been used for...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2021-03-01
|
| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/bjmg-2020-0020 |
| _version_ | 1797760927199657984 |
|---|---|
| author | Ikbal Atli E Gurkan H Onur Kirkizlar H Atli E Demir S Yalcintepe S Kalkan R Demir AM |
| author_facet | Ikbal Atli E Gurkan H Onur Kirkizlar H Atli E Demir S Yalcintepe S Kalkan R Demir AM |
| author_sort | Ikbal Atli E |
| collection | DOAJ |
| description | Multiple myeloma (MM) is one of the plasma cell-related hematological malignancies exceeding 10.0% of all marrow cells, and they make a paraprotein that is a marker of the disease. Myeloma is one of the most common types of hematological malignancies in humans. Genetic bio-markers have been used for prognostic markers in patients diagnosed with MM. The genetic and genomic changes have been identified using karyotyping, fluorescent in situ hybridization (FISH), next generation sequencing (NGS), specifically whole-genome sequencing or exome sequencing. Circulatory plasma cells, circulating free DNA (cfD-NA) and microRNAs (miRNAs) comprised in liquid biopsy are potentially used in diagnosis/prognosis of MM. In this study, we analyzed and compared results of karyo-typing, FISH and NGS in 35 MM cases. Diagnostic strategies are expanding rapidly and newly developed NGS-based testing may help the understanding of the complexities of genetic alterations in karyotypically normal cases. |
| first_indexed | 2024-03-12T19:05:35Z |
| format | Article |
| id | doaj.art-c43383aad31647ac81898551345cca6b |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T19:05:35Z |
| publishDate | 2021-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-c43383aad31647ac81898551345cca6b2023-08-02T06:19:00ZengSciendoBalkan Journal of Medical Genetics1311-01602021-03-01232596410.2478/bjmg-2020-0020Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myelomaIkbal Atli E0Gurkan H1Onur Kirkizlar H2Atli E3Demir S4Yalcintepe S5Kalkan R6Demir AM7Department of Medical Genetics, Faculty of Medicine, Edirne, Trakya University, Edirne, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Edirne, Trakya University, Edirne, TurkeyDepartment of Hematology, Faculty of Medicine, Trakya University, Edirne, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Edirne, Trakya University, Edirne, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Edirne, Trakya University, Edirne, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Edirne, Trakya University, Edirne, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Near East University, North Nicosia, Northern CyprusDepartment of Hematology, Faculty of Medicine, Trakya University, Edirne, TurkeyMultiple myeloma (MM) is one of the plasma cell-related hematological malignancies exceeding 10.0% of all marrow cells, and they make a paraprotein that is a marker of the disease. Myeloma is one of the most common types of hematological malignancies in humans. Genetic bio-markers have been used for prognostic markers in patients diagnosed with MM. The genetic and genomic changes have been identified using karyotyping, fluorescent in situ hybridization (FISH), next generation sequencing (NGS), specifically whole-genome sequencing or exome sequencing. Circulatory plasma cells, circulating free DNA (cfD-NA) and microRNAs (miRNAs) comprised in liquid biopsy are potentially used in diagnosis/prognosis of MM. In this study, we analyzed and compared results of karyo-typing, FISH and NGS in 35 MM cases. Diagnostic strategies are expanding rapidly and newly developed NGS-based testing may help the understanding of the complexities of genetic alterations in karyotypically normal cases.https://doi.org/10.2478/bjmg-2020-0020cytogeneticsfluorescent in situ hybridization (fish)multiple myelomanext generation sequencing (ngs) |
| spellingShingle | Ikbal Atli E Gurkan H Onur Kirkizlar H Atli E Demir S Yalcintepe S Kalkan R Demir AM Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma Balkan Journal of Medical Genetics cytogenetics fluorescent in situ hybridization (fish) multiple myeloma next generation sequencing (ngs) |
| title | Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma |
| title_full | Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma |
| title_fullStr | Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma |
| title_full_unstemmed | Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma |
| title_short | Pros and cons for fluorescent in situ hybridization, karyotyping and next generation sequencing for diagnosis and follow-up of multiple myeloma |
| title_sort | pros and cons for fluorescent in situ hybridization karyotyping and next generation sequencing for diagnosis and follow up of multiple myeloma |
| topic | cytogenetics fluorescent in situ hybridization (fish) multiple myeloma next generation sequencing (ngs) |
| url | https://doi.org/10.2478/bjmg-2020-0020 |
| work_keys_str_mv | AT ikbalatlie prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT gurkanh prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT onurkirkizlarh prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT atlie prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT demirs prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT yalcintepes prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT kalkanr prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma AT demiram prosandconsforfluorescentinsituhybridizationkaryotypingandnextgenerationsequencingfordiagnosisandfollowupofmultiplemyeloma |