Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
Abstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discrim...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07244-2 |
| _version_ | 1818840924361326592 |
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| author | Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii |
| author_facet | Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii |
| author_sort | Takahiro Matsui |
| collection | DOAJ |
| description | Abstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were ‘conventional’ images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images – the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) – rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness. |
| first_indexed | 2024-12-19T04:17:55Z |
| format | Article |
| id | doaj.art-c43ac4bddec64a5baad9827194f4cbb0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:17:55Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c43ac4bddec64a5baad9827194f4cbb02022-12-21T20:36:15ZengNature PortfolioScientific Reports2045-23222017-07-01711910.1038/s41598-017-07244-2Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesionsTakahiro Matsui0Hiroki Mizuno1Takao Sudo2Junichi Kikuta3Naotsugu Haraguchi4Jun-ichiro Ikeda5Tsunekazu Mizushima6Hirofumi Yamamoto7Eiichi Morii8Masaki Mori9Masaru Ishii10Department of Immunology and Cell Biology, Osaka University Graduate School of MedicineDepartment of Immunology and Cell Biology, Osaka University Graduate School of MedicineDepartment of Immunology and Cell Biology, Osaka University Graduate School of MedicineDepartment of Immunology and Cell Biology, Osaka University Graduate School of MedicineDepartment of Gastroenterological Surgery, Osaka University Graduate School of MedicineDepartment of Pathology, Osaka University Graduate School of MedicineDepartment of Gastroenterological Surgery, Osaka University Graduate School of MedicineDepartment of Gastroenterological Surgery, Osaka University Graduate School of MedicineDepartment of Pathology, Osaka University Graduate School of MedicineDepartment of Gastroenterological Surgery, Osaka University Graduate School of MedicineDepartment of Immunology and Cell Biology, Osaka University Graduate School of MedicineAbstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were ‘conventional’ images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images – the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) – rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.https://doi.org/10.1038/s41598-017-07244-2 |
| spellingShingle | Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions Scientific Reports |
| title | Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| title_full | Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| title_fullStr | Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| title_full_unstemmed | Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| title_short | Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| title_sort | non labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions |
| url | https://doi.org/10.1038/s41598-017-07244-2 |
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