Serum microRNA-126 expression as a biomarker of diabetic retinopathy
Background Diabetic retinopathy (DR) is a microvascular complication of diabetes mellitus (DM). Diabetic retinopathy causes permanent blindness in the productive age group and has a multifactorial pathogenesis. MicroRNA-126 (miRNA-126) regulates the expression of the vascular endothelial growth fac...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2023-06-01
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| Series: | Universa Medicina |
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| Online Access: | https://univmed.org/ejurnal/index.php/medicina/article/view/1445 |
| _version_ | 1797730002284838912 |
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| author | Ni Made Ayu Surasmiati Ni Made Ari Suryathi Ari Andayani |
| author_facet | Ni Made Ayu Surasmiati Ni Made Ari Suryathi Ari Andayani |
| author_sort | Ni Made Ayu Surasmiati |
| collection | DOAJ |
| description |
Background
Diabetic retinopathy (DR) is a microvascular complication of diabetes mellitus (DM). Diabetic retinopathy causes permanent blindness in the productive age group and has a multifactorial pathogenesis. MicroRNA-126 (miRNA-126) regulates the expression of the vascular endothelial growth factor (VEGF) gene at the post-transcriptional level, VEGF being an important angiogenic protein regulating inflammation in DR development. This study aimed to determine serum miRNA-126 expression as a biomarker in DM patients with DR.
Methods
This was a cross-sectional study involving 4 healthy persons and 21 type 2 DM patients. Subjects consisted of 4 groups: i) healthy controls, ii) DM patients without diabetic retinopathy (NDR), iii) DM patients with non-proliferative DR (NPDR) and iv) DM patients with proliferative DR (PDR). Venous blood was collected from subjects for miRNA-126 examination by real-time polymerase chain reaction (PCR). MiRNA-126 in each group was analyzed using the One Way Anova test and p<0.05 was considered to be statistically significant.
Results
Mean miRNA-126 expression was significantly decreased in PDR (1.86±1.03) and NPDR (1.01±0.43 ) groups when compared to healthy control (2.44±1.29) and NDR groups (2.15± 0.48) (p=0.027). MiRNA-126 values of less than 1.81 can differentiate NDR from the control group (sensitivity 83%, specificity 75%) and miRNA-126 of less than 1.56 can be used to predict NPDR when compared to the control group (sensitivity 86%, specificity 75%).
Conclusion
Serum miRNA-126 is a potential biomarker for screening of NPDR and NDR in type 2 DM patients, and could be considered a non-invasive diagnostic parameter.
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| first_indexed | 2024-03-12T11:38:51Z |
| format | Article |
| id | doaj.art-c43afc5a3fcd4a60aeb415ac9ad7c41c |
| institution | Directory Open Access Journal |
| issn | 1907-3062 2407-2230 |
| language | English |
| last_indexed | 2024-03-12T11:38:51Z |
| publishDate | 2023-06-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-c43afc5a3fcd4a60aeb415ac9ad7c41c2023-09-01T02:20:23ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302023-06-0142210.18051/UnivMed.2023.v42.121-127Serum microRNA-126 expression as a biomarker of diabetic retinopathyNi Made Ayu Surasmiati0Ni Made Ari Suryathi1Ari Andayani2Ophthalmology Department, Faculty of Medicine, Udayana University, Bali, IndonesiaOphthalmology Department, Faculty of Medicine, Udayana University, Bali, IndonesiaOphthalmology Department, Faculty of Medicine, Udayana University, Bali, Indonesia Background Diabetic retinopathy (DR) is a microvascular complication of diabetes mellitus (DM). Diabetic retinopathy causes permanent blindness in the productive age group and has a multifactorial pathogenesis. MicroRNA-126 (miRNA-126) regulates the expression of the vascular endothelial growth factor (VEGF) gene at the post-transcriptional level, VEGF being an important angiogenic protein regulating inflammation in DR development. This study aimed to determine serum miRNA-126 expression as a biomarker in DM patients with DR. Methods This was a cross-sectional study involving 4 healthy persons and 21 type 2 DM patients. Subjects consisted of 4 groups: i) healthy controls, ii) DM patients without diabetic retinopathy (NDR), iii) DM patients with non-proliferative DR (NPDR) and iv) DM patients with proliferative DR (PDR). Venous blood was collected from subjects for miRNA-126 examination by real-time polymerase chain reaction (PCR). MiRNA-126 in each group was analyzed using the One Way Anova test and p<0.05 was considered to be statistically significant. Results Mean miRNA-126 expression was significantly decreased in PDR (1.86±1.03) and NPDR (1.01±0.43 ) groups when compared to healthy control (2.44±1.29) and NDR groups (2.15± 0.48) (p=0.027). MiRNA-126 values of less than 1.81 can differentiate NDR from the control group (sensitivity 83%, specificity 75%) and miRNA-126 of less than 1.56 can be used to predict NPDR when compared to the control group (sensitivity 86%, specificity 75%). Conclusion Serum miRNA-126 is a potential biomarker for screening of NPDR and NDR in type 2 DM patients, and could be considered a non-invasive diagnostic parameter. https://univmed.org/ejurnal/index.php/medicina/article/view/1445microRNA-126diabetes mellitusdiabetic retinopathybiomarker |
| spellingShingle | Ni Made Ayu Surasmiati Ni Made Ari Suryathi Ari Andayani Serum microRNA-126 expression as a biomarker of diabetic retinopathy Universa Medicina microRNA-126 diabetes mellitus diabetic retinopathy biomarker |
| title | Serum microRNA-126 expression as a biomarker of diabetic retinopathy |
| title_full | Serum microRNA-126 expression as a biomarker of diabetic retinopathy |
| title_fullStr | Serum microRNA-126 expression as a biomarker of diabetic retinopathy |
| title_full_unstemmed | Serum microRNA-126 expression as a biomarker of diabetic retinopathy |
| title_short | Serum microRNA-126 expression as a biomarker of diabetic retinopathy |
| title_sort | serum microrna 126 expression as a biomarker of diabetic retinopathy |
| topic | microRNA-126 diabetes mellitus diabetic retinopathy biomarker |
| url | https://univmed.org/ejurnal/index.php/medicina/article/view/1445 |
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