Porcine Germ Cells Phenotype during Embryonic and Adult Development
Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals,...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/15/2520 |
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| author | Amanda Soares Jorge Kaiana Recchia Mayra Hirakawa Glória Aline Fernanda de Souza Laís Vicari de Figueirêdo Pessôa Paulo Fantinato Neto Daniele dos Santos Martins André Furugen Cesar de Andrade Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Naira Caroline Godoy Pieri |
| author_facet | Amanda Soares Jorge Kaiana Recchia Mayra Hirakawa Glória Aline Fernanda de Souza Laís Vicari de Figueirêdo Pessôa Paulo Fantinato Neto Daniele dos Santos Martins André Furugen Cesar de Andrade Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Naira Caroline Godoy Pieri |
| author_sort | Amanda Soares Jorge |
| collection | DOAJ |
| description | Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro. |
| first_indexed | 2024-03-11T00:32:36Z |
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| id | doaj.art-c43c9f6c313a4d92af5f3771764c3b3e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T00:32:36Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c43c9f6c313a4d92af5f3771764c3b3e2023-11-18T22:34:11ZengMDPI AGAnimals2076-26152023-08-011315252010.3390/ani13152520Porcine Germ Cells Phenotype during Embryonic and Adult DevelopmentAmanda Soares Jorge0Kaiana Recchia1Mayra Hirakawa Glória2Aline Fernanda de Souza3Laís Vicari de Figueirêdo Pessôa4Paulo Fantinato Neto5Daniele dos Santos Martins6André Furugen Cesar de Andrade7Simone Maria Massami Kitamura Martins8Fabiana Fernandes Bressan9Naira Caroline Godoy Pieri10Department of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of Sao Paulo, São Paulo 01001-010, SP, BrazilDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment Biomedical Science, Ontario Veterinary College (OVC), University of Guelph, Guelph, ON N1G 2W1, CanadaDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Animal Reproduction, Faculty of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Animal Production, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, BrazilPrimordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro.https://www.mdpi.com/2076-2615/13/15/2520PGCsreproductionporcine |
| spellingShingle | Amanda Soares Jorge Kaiana Recchia Mayra Hirakawa Glória Aline Fernanda de Souza Laís Vicari de Figueirêdo Pessôa Paulo Fantinato Neto Daniele dos Santos Martins André Furugen Cesar de Andrade Simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan Naira Caroline Godoy Pieri Porcine Germ Cells Phenotype during Embryonic and Adult Development Animals PGCs reproduction porcine |
| title | Porcine Germ Cells Phenotype during Embryonic and Adult Development |
| title_full | Porcine Germ Cells Phenotype during Embryonic and Adult Development |
| title_fullStr | Porcine Germ Cells Phenotype during Embryonic and Adult Development |
| title_full_unstemmed | Porcine Germ Cells Phenotype during Embryonic and Adult Development |
| title_short | Porcine Germ Cells Phenotype during Embryonic and Adult Development |
| title_sort | porcine germ cells phenotype during embryonic and adult development |
| topic | PGCs reproduction porcine |
| url | https://www.mdpi.com/2076-2615/13/15/2520 |
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