Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors
Manufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/6284 |
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| author | Mariana Cañas-Arboleda Karl Beltrán Carlos Medina Bernardo Camacho Gustavo Salguero |
| author_facet | Mariana Cañas-Arboleda Karl Beltrán Carlos Medina Bernardo Camacho Gustavo Salguero |
| author_sort | Mariana Cañas-Arboleda |
| collection | DOAJ |
| description | Manufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a feasible alternative for cell growth supplement. Nevertheless, composition and functional characterization of hPL in the context of cell manufacturing is still under investigation, particularly regarding the content and function of pro-survival and pro-regenerative factors. We performed comparative analyses of hPL, human serum (hS) and fetal bovine serum (FBS) stability and potency to support Wharton’s jelly (WJ) MSC production. We demonstrated that hPL displayed low inter-batch variation and unique secretome profile that was not present in hS and FBS. Importantly, hPL-derived factors including PDGF family, EGF, TGF-alpha, angiogenin and RANTES were actively taken up by WJ-MSC to support efficient expansion. Moreover, hPL but not hS or FBS induced secretion of osteoprotegerin, HGF, IL-6 and GRO-alpha by WJ-MSC during the expansion phase. Thus, hPL is a suitable source of factors supporting viability, stability and potency of WJ-MSC and therefore constitutes an essential raw material that in combination with WJ-MSC introduces a great opportunity for the generation of potent regenerative medicine products. |
| first_indexed | 2024-03-10T16:42:18Z |
| format | Article |
| id | doaj.art-c43dab52dcfe4113a1c2f5dd3ad7e48c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:42:18Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c43dab52dcfe4113a1c2f5dd3ad7e48c2023-11-20T11:56:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117628410.3390/ijms21176284Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative FactorsMariana Cañas-Arboleda0Karl Beltrán1Carlos Medina2Bernardo Camacho3Gustavo Salguero4Advanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud—IDCBIS, 111611 Bogotá, ColombiaAdvanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud—IDCBIS, 111611 Bogotá, ColombiaAdvanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud—IDCBIS, 111611 Bogotá, ColombiaAdvanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud—IDCBIS, 111611 Bogotá, ColombiaAdvanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud—IDCBIS, 111611 Bogotá, ColombiaManufacturing of mesenchymal stromal cell (MSC)-based therapies for regenerative medicine requires the use of suitable supply of growth factors that enhance proliferation, cell stability and potency during cell expansion. Human blood derivatives such as human platelet lysate (hPL) have emerged as a feasible alternative for cell growth supplement. Nevertheless, composition and functional characterization of hPL in the context of cell manufacturing is still under investigation, particularly regarding the content and function of pro-survival and pro-regenerative factors. We performed comparative analyses of hPL, human serum (hS) and fetal bovine serum (FBS) stability and potency to support Wharton’s jelly (WJ) MSC production. We demonstrated that hPL displayed low inter-batch variation and unique secretome profile that was not present in hS and FBS. Importantly, hPL-derived factors including PDGF family, EGF, TGF-alpha, angiogenin and RANTES were actively taken up by WJ-MSC to support efficient expansion. Moreover, hPL but not hS or FBS induced secretion of osteoprotegerin, HGF, IL-6 and GRO-alpha by WJ-MSC during the expansion phase. Thus, hPL is a suitable source of factors supporting viability, stability and potency of WJ-MSC and therefore constitutes an essential raw material that in combination with WJ-MSC introduces a great opportunity for the generation of potent regenerative medicine products.https://www.mdpi.com/1422-0067/21/17/6284Wharton’s Jelly Mesenchymal Stromal Cellshuman platelet lysatecytokineshuman growth factors |
| spellingShingle | Mariana Cañas-Arboleda Karl Beltrán Carlos Medina Bernardo Camacho Gustavo Salguero Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors International Journal of Molecular Sciences Wharton’s Jelly Mesenchymal Stromal Cells human platelet lysate cytokines human growth factors |
| title | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
| title_full | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
| title_fullStr | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
| title_full_unstemmed | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
| title_short | Human Platelet Lysate Supports Efficient Expansion and Stability of Wharton’s Jelly Mesenchymal Stromal Cells via Active Uptake and Release of Soluble Regenerative Factors |
| title_sort | human platelet lysate supports efficient expansion and stability of wharton s jelly mesenchymal stromal cells via active uptake and release of soluble regenerative factors |
| topic | Wharton’s Jelly Mesenchymal Stromal Cells human platelet lysate cytokines human growth factors |
| url | https://www.mdpi.com/1422-0067/21/17/6284 |
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