Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey
Introduction: Detecting Pseudomonas aeruginosa strains producing extended-spectrum beta-lactamase (ESBL) in the hospital setting and taking necessary precautions against them is important for infection control and public health. This study aimed to investigate PER-1 and OXA-10-like ESBLs productio...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
2022-03-01
|
| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-01/87-93%20Enis%20Fuat%20Tufekci.pdf |
| _version_ | 1797906050922315776 |
|---|---|
| author | Sarah AKAR Enis Fuat TÜFEKCİ Çetin KILINÇ Yasemin ÇELİK ALTUNOĞLU Mehmet Cengiz BALOĞLU Nilay ÇÖPLÜ |
| author_facet | Sarah AKAR Enis Fuat TÜFEKCİ Çetin KILINÇ Yasemin ÇELİK ALTUNOĞLU Mehmet Cengiz BALOĞLU Nilay ÇÖPLÜ |
| author_sort | Sarah AKAR |
| collection | DOAJ |
| description | Introduction: Detecting Pseudomonas aeruginosa strains producing extended-spectrum beta-lactamase (ESBL) in the hospital setting
and taking necessary precautions against them is important for infection control and public health. This study aimed to investigate
PER-1 and OXA-10-like ESBLs production frequency in ceftazidime-resistant P. aeruginosa obtained from Kastamonu Training and
Research Hospital.
Materials and Methods: Forty-two ceftazidime-resistant P. aeruginosa strains from different patients between April 2018 and March
2020 were included in the study. Identification of the strains and antibiotic susceptibility tests were studied according to the European
Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria by VITEK 2 Compact automated system (BioMerieux, France).
ESBL production of the strains was studied by combined disk test, phenotypically. The presence of PER-1 and OXA-10-like genes was
investigated by polymerase chain reaction (PCR). Confirmation of the PCR product was done using DNA sequencing on an ABI PRISM
3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).
Results: The resistance rates of the strains were: 19%, 81%, 88%, 88%, 91%, 95%, and 95% for amikacin, gentamicin, meropenem,
cefepime, imipenem, piperacillin-tazobactam, and ciprofloxacin, respectively. Twenty-seven (64%) strains had the ESBL phenotype.
Only one strain had PER-1 gene. OXA-10-like gene was not found in any strains. PER-1 sequence was identical and corresponded to
the published sequences for PER-1 gene in GenBank at the National Center for Biotechnology Information.
Conclusion: These results showed that PER-1 and OXA-10-like genes were not common among ceftazidime-resistant P. aeruginosa
strains obtained from our hospital. ESBL production can be determined phenotypically in ceftazidime-resistant P. aeruginosa strains.
However, confirming the results with molecular tests is significant for epidemiological studies. |
| first_indexed | 2024-04-10T10:14:54Z |
| format | Article |
| id | doaj.art-c43ef69d2ff9407ca586e4b953da67c2 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T10:14:54Z |
| publishDate | 2022-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-c43ef69d2ff9407ca586e4b953da67c22023-02-15T16:21:56ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-03-01271879310.5578/flora.20229907Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, TurkeySarah AKAR0https://orcid.org/0000-0002-5835-7315Enis Fuat TÜFEKCİ1https://orcid.org/0000-0001-5051-2694Çetin KILINÇ2https://orcid.org/0000-0003-4958-2622Yasemin ÇELİK ALTUNOĞLU3https://orcid.org/0000-0003-2940-7464Mehmet Cengiz BALOĞLU4https://orcid.org/0000-0003-2976-7224Nilay ÇÖPLÜ5https://orcid.org/0000-0003-1956-1417Department of Genetics and Bioengineering, Kastamonu University Faculty of Engineering and Architecture, Kastamonu, TurkeyDepartment of Medical Microbiology, Kastamonu University Faculty of Medicine, Kastamonu, TurkeyMicrobiology Laboratory, Kastamonu Training and Research Hospital, Kastamonu, TurkeyDepartment of Genetics and Bioengineering, Kastamonu University Faculty of Engineering and Architecture, Kastamonu, TurkeyDepartment of Genetics and Bioengineering, Kastamonu University Faculty of Engineering and Architecture, Kastamonu, TurkeyDepartment of Medical Microbiology, Kastamonu University Faculty of Medicine, Kastamonu, TurkeyIntroduction: Detecting Pseudomonas aeruginosa strains producing extended-spectrum beta-lactamase (ESBL) in the hospital setting and taking necessary precautions against them is important for infection control and public health. This study aimed to investigate PER-1 and OXA-10-like ESBLs production frequency in ceftazidime-resistant P. aeruginosa obtained from Kastamonu Training and Research Hospital. Materials and Methods: Forty-two ceftazidime-resistant P. aeruginosa strains from different patients between April 2018 and March 2020 were included in the study. Identification of the strains and antibiotic susceptibility tests were studied according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria by VITEK 2 Compact automated system (BioMerieux, France). ESBL production of the strains was studied by combined disk test, phenotypically. The presence of PER-1 and OXA-10-like genes was investigated by polymerase chain reaction (PCR). Confirmation of the PCR product was done using DNA sequencing on an ABI PRISM 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Results: The resistance rates of the strains were: 19%, 81%, 88%, 88%, 91%, 95%, and 95% for amikacin, gentamicin, meropenem, cefepime, imipenem, piperacillin-tazobactam, and ciprofloxacin, respectively. Twenty-seven (64%) strains had the ESBL phenotype. Only one strain had PER-1 gene. OXA-10-like gene was not found in any strains. PER-1 sequence was identical and corresponded to the published sequences for PER-1 gene in GenBank at the National Center for Biotechnology Information. Conclusion: These results showed that PER-1 and OXA-10-like genes were not common among ceftazidime-resistant P. aeruginosa strains obtained from our hospital. ESBL production can be determined phenotypically in ceftazidime-resistant P. aeruginosa strains. However, confirming the results with molecular tests is significant for epidemiological studies.http://floradergisi.org/managete/fu_folder/2022-01/87-93%20Enis%20Fuat%20Tufekci.pdfceftazidimeoxa-10-likeper-1pcrpseudomonas aeruginosa |
| spellingShingle | Sarah AKAR Enis Fuat TÜFEKCİ Çetin KILINÇ Yasemin ÇELİK ALTUNOĞLU Mehmet Cengiz BALOĞLU Nilay ÇÖPLÜ Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi ceftazidime oxa-10-like per-1 pcr pseudomonas aeruginosa |
| title | Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey |
| title_full | Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey |
| title_fullStr | Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey |
| title_full_unstemmed | Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey |
| title_short | Determination of PER-1 and OXA-10-like ExtendedSpectrum Beta-Lactamases Frequency in CeftazidimeResistant Pseudomonas aeruginosa Strains Isolated in Kastamonu Training and Research Hospital, Turkey |
| title_sort | determination of per 1 and oxa 10 like extendedspectrum beta lactamases frequency in ceftazidimeresistant pseudomonas aeruginosa strains isolated in kastamonu training and research hospital turkey |
| topic | ceftazidime oxa-10-like per-1 pcr pseudomonas aeruginosa |
| url | http://floradergisi.org/managete/fu_folder/2022-01/87-93%20Enis%20Fuat%20Tufekci.pdf |
| work_keys_str_mv | AT sarahakar determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey AT enisfuattufekci determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey AT cetinkilinc determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey AT yasemincelikaltunoglu determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey AT mehmetcengizbaloglu determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey AT nilaycoplu determinationofper1andoxa10likeextendedspectrumbetalactamasesfrequencyinceftazidimeresistantpseudomonasaeruginosastrainsisolatedinkastamonutrainingandresearchhospitalturkey |