Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels
The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood....
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2077-0472/13/10/1909 |
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| author | Ahmed Ateya Fatmah A. Safhi Huda El-Emam Marawan A. Marawan Hayat Fayed Amgad Kadah Maha Mamdouh Manar M. Hizam Muath Q. Al-Ghadi Mohamed Abdo Liana Fericean Rada Olga Ostan Mihaela |
| author_facet | Ahmed Ateya Fatmah A. Safhi Huda El-Emam Marawan A. Marawan Hayat Fayed Amgad Kadah Maha Mamdouh Manar M. Hizam Muath Q. Al-Ghadi Mohamed Abdo Liana Fericean Rada Olga Ostan Mihaela |
| author_sort | Ahmed Ateya |
| collection | DOAJ |
| description | The immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants to obtain whole blood and extract DNA and RNA. The immunological (<i>OTUD3</i>, <i>TLR2</i>, <i>TLR4</i>, <i>STAB2</i>, <i>MBL2</i>, <i>TRAPPC9</i>, and <i>C4A</i>) and antioxidant (<i>CAT</i>, <i>SOD3</i>, <i>PRDX6</i>, <i>OXSR1</i>, <i>NDUFS6</i>, <i>SERP2</i>, and <i>ST1P1</i>) genes’ nucleotide sequence polymorphisms between healthy and mastitis affected she-camels were discovered using PCR-DNA sequencing. Fisher’s exact test revealed that camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations propagating (<i>p</i> < 0.01). Mastitic camels were significantly more likely to express the <i>OTUD3</i>, <i>TLR2</i>, <i>TLR4</i>, <i>STAB2</i>, <i>MBL2</i>, <i>TRAPPC9</i>, <i>C4A</i>, <i>OXSR1</i>, <i>SERP2</i>, and <i>ST1P1</i> genes (<i>p</i> < 0.05). However, <i>CAT</i>, <i>SOD3</i>, <i>PRDX6</i>, and <i>NDUFS6</i> genes elicited a different pattern. The results may be used to develop management strategies and support the significance of nucleotide differences and gene expression patterns in these markers as indicators of the incidence of mastitis. |
| first_indexed | 2024-03-10T21:31:41Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T21:31:41Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-c444e704696a4dcba278f09901acf1f12023-11-19T15:18:29ZengMDPI AGAgriculture2077-04722023-09-011310190910.3390/agriculture13101909Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary CamelsAhmed Ateya0Fatmah A. Safhi1Huda El-Emam2Marawan A. Marawan3Hayat Fayed4Amgad Kadah5Maha Mamdouh6Manar M. Hizam7Muath Q. Al-Ghadi8Mohamed Abdo9Liana Fericean10Rada Olga11Ostan Mihaela12Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Benha 13511, EgyptDepartment of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Benha University, Benha 13511, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, EgyptCollege of Pharmacy, National University of Science and Technology, Nasiriyah 64001, Dhi Qar, IraqDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Animal Histology and Anatomy, School of Veterinary Medicine, Badr University in Cairo (BUC), Cairo 11829, EgyptDepartment of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, RomaniaDepartment of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, RomaniaDepartment of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, RomaniaThe immune and antioxidant genetic factors that could converse with mastitis susceptibility in dromedary camels were looked at in this research. Of 120 female dromedary camels (60 healthy, and 60 with mastitis) were utilised. Each camel’s jugular vein was pierced to obtain five millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants to obtain whole blood and extract DNA and RNA. The immunological (<i>OTUD3</i>, <i>TLR2</i>, <i>TLR4</i>, <i>STAB2</i>, <i>MBL2</i>, <i>TRAPPC9</i>, and <i>C4A</i>) and antioxidant (<i>CAT</i>, <i>SOD3</i>, <i>PRDX6</i>, <i>OXSR1</i>, <i>NDUFS6</i>, <i>SERP2</i>, and <i>ST1P1</i>) genes’ nucleotide sequence polymorphisms between healthy and mastitis affected she-camels were discovered using PCR-DNA sequencing. Fisher’s exact test revealed that camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations propagating (<i>p</i> < 0.01). Mastitic camels were significantly more likely to express the <i>OTUD3</i>, <i>TLR2</i>, <i>TLR4</i>, <i>STAB2</i>, <i>MBL2</i>, <i>TRAPPC9</i>, <i>C4A</i>, <i>OXSR1</i>, <i>SERP2</i>, and <i>ST1P1</i> genes (<i>p</i> < 0.05). However, <i>CAT</i>, <i>SOD3</i>, <i>PRDX6</i>, and <i>NDUFS6</i> genes elicited a different pattern. The results may be used to develop management strategies and support the significance of nucleotide differences and gene expression patterns in these markers as indicators of the incidence of mastitis.https://www.mdpi.com/2077-0472/13/10/1909immunityantioxidant biomarkerstranscript levelsmastitisdromedary camels |
| spellingShingle | Ahmed Ateya Fatmah A. Safhi Huda El-Emam Marawan A. Marawan Hayat Fayed Amgad Kadah Maha Mamdouh Manar M. Hizam Muath Q. Al-Ghadi Mohamed Abdo Liana Fericean Rada Olga Ostan Mihaela Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels Agriculture immunity antioxidant biomarkers transcript levels mastitis dromedary camels |
| title | Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels |
| title_full | Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels |
| title_fullStr | Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels |
| title_full_unstemmed | Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels |
| title_short | Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels |
| title_sort | combining nucleotide sequence variants and transcript levels of immune and antioxidant markers for selection and improvement of mastitis resistance in dromedary camels |
| topic | immunity antioxidant biomarkers transcript levels mastitis dromedary camels |
| url | https://www.mdpi.com/2077-0472/13/10/1909 |
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